The Mediterranean Diet (Med Diet) has long been shown to be associated with lower cardiovascular mortality in epidemiological studies. However, the direct effect of the dietary pattern on cardiovascular risk factors is less clear. Furthermore, the effect of Med Diet interventions in non-Mediterranean populations on cardiovascular and metabolic risk is variable. A Cochrane Review in 2019 concluded there was low to moderate evidence of a modest benefit but noted that there were still ongoing trials. Since 2019, there have been a number of published trials that have not shown a benefit of the Med Diet over other interventions or usual care. It is possible that dietary factors such as quality or quantity of carbohydrate and energy restriction are more important factors, along with the degree of weight loss, than the Med Diet itself. There are also many other factors that influence the uptake and effectiveness of the Med Diet in populations that have a different traditional or habitual diet. This review paper examines a selection of 20 Med Diet intervention trials specifically looking at clinical outcomes of glucose metabolism: fasting plasma glucose, HbA1c, development of type 2 diabetes or need for hypoglycaemic medications, with or without other cardiometabolic risk factors. These trials are a mix of randomised controlled trials, crossover studies and cohort studies of greater than 8 weeks duration with more than 25 participants. There is heterogeneity in study designs and outcomes, making comparison difficult, but there is no clear benefit of the Med Diet presented.

Ring sea anemones (Cnidaria, Actiniaria) engage in symbiotic associations with octocorals by attaching to their branches and surrounding them with tissue junctions, causing a significant reduction of the host’s coenenchyme. They have previously been reported from the North Atlantic, Western Indian, and Pacific Oceans, from 30 m to aphotic depths, colonising species of sea fans and sea pens in potentially parasitic relationships. Here, we report the first record of ring sea anemones from the Saudi Arabian Red Sea mesophotic waters, living on an octocoral host of the family Ellisellidae at 97 m depth. Through morphological and phylogenetic analyses based on the sequencing of the 12S small subunit rRNA region, we confirmed the identification of these ring sea anemones as Peronanthus sp. (Actiniaria, Amphianthidae). While expanding our knowledge on the geographic range of this genus, this finding emphasises the importance of explorations of coral-associated fauna at mesophotic depths, to better understand their diversity, their relationships with their hosts, and their potential ecological roles within these ecosystems.

Armenia is notable for its wild pear diversity, which includes a large number of globally threatened endemic species. Despite progress in recent studies there still remains, however, a lack of data on the distribution and population sizes of some species, and any threats to them. This information is necessary to assess their conservation status and for the planning and implementation of conservation actions. I present the results of fieldwork in the Vayots Dzor province of Armenia on the globally threatened endemic pear species Pyrus daralagezi Mulk., Pyrus hajastana Mulk. and Pyrus gergerana Gladkova. I report new data on distribution, threats and species biology. I inventoried individual trees, discovering five new individuals of P. daralagezi in Herher sanctuary, rediscovering a population of P. hajastana in its locus classicus 52 years after the first record, and finding a new locality for P. gergerana around the village of Artavan. I make recommendations for the further study and conservation of these threatened pear species.

Time-resolved (TR) intrinsic fluorescence of tryptophan (Trp) provides a wealth of information on the structure and localization of proteins and peptides and their interactions with one another, with drugs, lipid membranes, lipid- and surfactant-based drug delivery systems, et cetera. Intrinsic Trp eliminates the need for labeling and avoids the perturbation of the system by the label; introduced Trp is a rather conservative and small label compared to others. Whereas custom-tailored fluorophores are often optimized for a special technique, Trp can be employed to monitor a wide variety of effects. We address interactions of Trp with surrounding molecules, dynamic quenchers and Förster resonance energy transfer (FRET) acceptors that affect the fluorescence decay. Speed and range of angular motion of Trp are characterized by TR anisotropy. Electrostatic interactions of Trp with charged and polar molecules, including water, are monitored by decay-associated spectra (DAS) or TR emission spectra (TRES) and quantified in terms of TR shifts of the spectral center of gravity. This versatility is a great advantage and, at the same time, comes with a complexity of the behavior that can render it a challenge to interpret the data in detail properly. This review provides an overview of applications of TR fluorescence of Trp bulk samples in biomolecular, biophysical, and pharmaceutical studies. The aim is not only to point out the diversity of the read-out of these techniques, but also critically examine their current use. Therefore, we identify most common technical pitfalls and evaluate the degree of reliability of the interpretational approaches. This should aid a more extensive and meaningful use of TR fluorescence of Trp.

This study, conducted under semi-arid conditions during the 2022 and 2023 growing seasons, aimed to assess the effects of plant growth-promoting rhizobacteria (PGPR) in combination with different irrigation levels and water qualities on safflower (Carthamus tinctorius L.) quality parameters. The irrigation levels were based on 0% (I0 - rainfed), 25% (I25), 50% (I50), 75% (I75) and 100% (I100) of Class A pan evaporation. Two irrigation water qualities were used: recycled wastewater (RW) and freshwater (FW). The PGPR treatments were applied at four frequencies: R0 (control), R1 (once), R2 (twice) and R3 (three times) starting after sowing at 10-day intervals. Seed protein content ranged from 12.0% to 18.1%, with the highest values under I100-R3 and the lowest under I0-R0. Protein content increased with irrigation and bacterial application frequency. Oil content varied between 25.2% and 38.6%, peaking under full irrigation with triple PGPR application (I100-R3), and was generally higher in RW-irrigated plots. SPAD (Soil Plant Analysis Development) values which are an indication for chlorophyll content in the plant ranged from 45.1 to 76.3, with RW-I100 treatments showing the highest readings. Stomatal conductance values varied between 40.5 and 122.0 mmol/m2/s¹, increasing with irrigation level. Overall, combining recycled wastewater and PGPR under sufficient irrigation significantly improved safflower’s physiological and biochemical characteristics. The results suggest that this integrated approach enhances oil and protein content while supporting sustainable water use and crop quality improvement in arid and semi-arid regions.

The current study investigates farmers’ behavioral intentions toward adopting biopesticides in India, integrating the theory of planned behavior and health belief model. The models were further expanded by the inclusion of a control variable, Government Initiatives. A total of 468 responses from four districts (Patna, Bhagalpur, Purnea, and Saharsa) in Bihar were collected by face-to-face surveys and analyzed using the SmartPLS 4 software by structural equation modeling to assess the correlation between the constructs. The hypothesis testing employs a bootstrapping method with 5,000 iterations. The present study demonstrated a strong positive correlation between all variables derived in the integrated model. Perceived severity strongly influenced farmers’ attitudes toward adopting the use of biopesticide. Furthermore, subjective norms and government initiatives emerged as the most important factors influencing farmers’ intentions toward adopting biopesticide. Premium price, low effectiveness, and reduced crop productivity emerged as significant challenges to the adoption of biopesticides. To address these challenges, this study suggests providing farmers with affordable solutions and resources in collaboration with the government. The proposed study provides significant information and facilitates the understanding of farmers’ inclinations to utilize biopesticides. The government and policymakers can address key barriers to the adoption of biopesticides—such as income inequality—by offering subsidies for organic food production, developing an efficient and dedicated supply chain for input and output organic produce, and formulating strategies to optimize the use of biopesticides in order to promote long-term sustainability.

Reducing methane emissions from livestock is crucial for agricultural sustainability, especially in the dairy sector, which emits high levels of methane. Seaweed-based supplements for livestock, like Asparagopsis taxiformis, offer promise in mitigating emissions from cattle without harming productivity, along with a range of other benefits. Little research to date has been conducted to understand how these supplements are being marketed and sold to dairy farms, and why they are marketed that way: this information can help ensure that these benefits will be realized. This study presents findings from a qualitative analysis of dairy feed suppliers in the Northeast United States, providing insights into their perspectives on seaweed supplements. It explores the differences between organic and conventional feed suppliers, including their perceived benefits and challenges of feeding seaweed. Feed suppliers stated that the benefits of feeding seaweed supplements include micromineral supplementation, reduced somatic cell count, reduced methane emissions, and decreased use of synthetic mineral supplements. Barriers to feeding seaweed include cost, the volume requirements of mills, supply inconsistencies, and a lack of investment from the conventional dairy industry. Feed suppliers also provided insights into what information they need about seaweed supplements to feel comfortable recommending them to their farmer customers, such as more scientific evidence and farmer recommendations. Understanding perceived benefits and barriers lays the foundation for further exploration of supply chain dynamics and stakeholder considerations in promoting sustainable livestock practices.

Zinc supplementation is a critical adjunct therapy for managing acute childhood diarrhoea, particularly in low-income countries (LICs) and lower middle-income countries (LMICs). However, adherence to the recommended zinc regimen remains a major challenge, limiting its effectiveness in real-world settings. This systematic review and meta-analysis aimed to estimate the pooled adherence rates to zinc supplementation for diarrhoea in children under 5 and identify key determinants of adherence. A comprehensive search of PubMed, Embase, Scopus, Google Scholar, ProQuest, and CINAHL was conducted between 2000 and 2024. A total of 10 observational studies were included, with pooled adherence of 63.45% (95% CI: 51.62–75.28) for 10 days regimen and 34.58% (95% CI: 7.08–62.09) for 14 days regimen, along with high heterogeneity. Sensitivity analysis confirmed robustness of these estimates. Key factors associated with adherence included caregiver education, provider counselling, medication acceptability, and economic constraints related to caregiver buying capacity. Doi plot asymmetry suggested possible publication bias for 10 and 14 days regimen. Overall, adherence to zinc therapy remains sub-optimal, particularly for 14 days regimen compared to 10 days regimen. Targeted interventions addressing behavioural, provider, and formulation related barriers are urgently needed to optimize zinc adherence and improve diarrhoea outcomes globally.

In this study, HFRS data were obtained from China CDC and ECDC, while monthly meteorological data and GDP were extracted from the National Bureau of Statistics of China website. Descriptive epidemiology, time series decomposition, and spatial autocorrelation analyses were employed to evaluate HFRS incidence patterns. A spatial panel data model was used to estimate the effects of meteorological and socio-economic variables on HFRS incidence. The average annual incidence rate of HFRS was 0.90/100000 in China, compared to 29.3/100000 in Finland. The incidence level in China was comparable to that in Belgium and the EU/EEA (excluding the UK), the high-incidence age group was 45–64 years, which was similar to Finland and the EU/EEA. HFRS in China exhibited marked seasonality. Three north-eastern provinces, Shaanxi, Shandong, and Jiangxi reported higher incidence rates. After adjusting for spatial individual effects and spatial autocorrelation, HFRS incidence was negatively associated with precipitation during the same period, per capita GDP showed no significant effect on HFRS incidence. Continued surveillance and prevention of HFRS remain necessary in China, particularly in Shaanxi. Additional disease prevention and control efforts should be directed towards individuals aged 45–64 years during the high-risk period from October to December.

Nutritional status has been compromised by ongoing war and restrictions on food deliveries in the Gaza Strip. We developed a mathematical model that outputs retrospective estimates and scenario-based projections of acute malnutrition prevalence among children given caloric intake and other factors. We present here the model and its application to the crisis in Gaza. We extended an existing mechanistic model for weight change as a function of energy balance, calibrating it to represent variability in growth curves observed in pre-war Gaza. We simulated open cohorts of children exposed to time-varying caloric intake, infant exclusive breast-feeding prevalence, incidence of infectious disease and coverage of malnutrition treatment, while allowing for adult caloric sacrifice to supplement child intake in times of food scarcity. The model accurately replicates growth standards, pre-war growth patterns and expected parameter dependencies. It suggests that a considerable increase in acute malnutrition occurred in northern Gaza during early 2024. Projections for late 2024 include a serious nutritional emergency if relatively pessimistic assumptions are made about food availability. The model may hold considerable promise for informing decisions in humanitarian response but requires further validation and development.

Agriculture is a major contributor to climate change, and there is an urgent need to reduce greenhouse gas (GHG) emissions from agriculture for mitigation purposes. Modern industrial agriculture has been recognized as a significant source of agricultural GHG emissions, whereas the adoption of regenerative organic agriculture has been proposed as a solution with the potential to reduce GHG emissions from agricultural production. However, there is a lack of on-the-ground studies reporting on the climate impacts of organic agriculture. To remedy this, a carbon footprint (CF) analysis was conducted comparing regionally representative organic and conventional arable cropping systems at Rodale Institute’s Farming Systems Trial in Pennsylvania, USA. Two separate modeling approaches were used to construct CFs for three agricultural systems (two organic and one conventional). The baseline CF analyses used an Intergovernmental Panel on Climate Change Tier 3 model (COMET-Farm) and Tier 2 model (Cool Farm Tool) for comparison purposes. Secondary analyses were conducted on the effects of CO2 emissions from composting manure on CFs. Emission metrics were generally higher (+27%) using the Tier 3 model compared with the Tier 2 model. In the baseline analysis, absolute area-scaled emissions were highest in the conventional system, ranging from 1.25 to 1.72 tons CO2-eq ha−1 yr−1. In comparison, emissions in the organic manure-based system were 25%–37% lower (0.94–1.09 tons CO2-eq ha−1 yr−1), while the organic legume-based system had the lowest emissions, which were 52%–74% lower (0.33–0.83 tons CO2-eq ha−1 yr−1). Yield-scaled emissions of maize in the baseline analyses were highest in the conventional system (0.19–0.26 kg CO2-eq kg−1), followed by the organic manure (0.13–0.16 kg CO2-eq kg−1) and organic legume (0.07–0.17 kg CO2-eq kg−1). Yield-scaled emissions on a feed digestible energy basis were highest in the conventional system (0.014–0.020 kg CO2-eq MJ−1) but were similar between organic manure (0.009–0.010 kg CO2-eq MJ−1) and organic legume (0.006–0.015 kg CO2-eq MJ−1). Including estimates of CO2 emissions due to composting increased emissions for the manure-based organic system substantially (+103%–122%). Our results imply that regenerative organic farming can help mitigate climate change. Future research should focus on more accurately measuring emissions from compost production and other sources of organic fertility, conducting a full life-cycle assessment of these systems, and verifying the results using in-situ field measurements.

Yellow toadflax is an invasive perennial broadleaf weed that negatively affects forage production and wildlife habitat. Few herbicides effectively manage this weed. Glyphosate is a nonselective herbicide that can help manage yellow toadflax but is rarely used in pasture/rangeland because it may injure desirable vegetation. The effectiveness of wiper-applied glyphosate followed by (fb) applications of either chlorsulfuron or picloram was evaluated on yellow toadflax grown in a greenhouse. Glyphosate was applied with a wiper at 0% or 50% diluted concentrate. Chlorsulfuron (26 g ai ha−1) was applied alone or immediately after the wiper application of glyphosate. Picloram (560 g ae ha−1) was applied in a similar manner. At 3 mo after herbicide treatments, estimates of injury to yellow toadflax were greatest with glyphosate, glyphosate fb chlorsulfuron, and glyphosate fb picloram (77% to 86%) compared with chlorsulfuron or picloram applied alone (52% to 56%). The response of subsequently harvested biomass was not different after herbicide treatments. One month after treatment, flowering and regrowth of biomass and root biomass were quantified. Flowering, regrowth, and root biomass demonstrated the greatest (>50%) recovery with wiper-applied glyphosate and wiper-applied glyphosate fb broadcast-applied chlorsulfuron or picloram compared with <50% recovery with broadcast applications of chlorsulfuron or picloram. The results of the experiment suggest that wiper-applied glyphosate fb broadcast applications of chlorsulfuron or picloram can help manage yellow toadflax regrowth compared with single herbicide treatments.