Haritalodes derogata (Fabricius) (Lepidoptera: Crambidae), commonly known as the cotton leaf roller, is an important polyphagous pest that causes damage to various agricultural and forest plants, especially those of the Malvaceae family, but also to crops such as cotton, cashew, bamboo, oats, and jute. While microbial control agents are known for their efficacy and environmental friendliness, there are few studies demonstrating their effect on H. derogata. The aim of this study was to evaluate the bioefficacy of microbial agents from different pathogen groups against this pest. To this end, we investigated the insecticidal potential of fifteen indigenous microbial isolates from our entomopathogen collection. These included five Bacillus thuringiensis strains (Bn1, MnD, Mm2, Xd3, Lyd8), five entomopathogenic fungi (Metarhizium flavoviride As2, M. anisopliae KTU-51 and Beauveria bassiana Pa4, Pa5, Hp5), and five baculoviruses (HycuGV-Hc1, LdMNPV-T2, AcMNPV, DapuNPV-T1, SeMNPV-U), applied at concentrations of 1.8 × 109 cfu/mL, 1 × 107 conidia/mL, and 1 × 107 PIB/mL, respectively, against H. derogata larvae under laboratory conditions. Among these, B. thuringiensis subsp. kurstaki Bn1, B. bassiana Pa4, Hyphantria cunea granulovirus (HycuGV-Hc1), and Spodoptera exigua nucleopolyhedrovirus (SeMNPV-U) showed strong insecticidal activity and were selected for virulence assays, each achieving 100% mortality in third instar larvae. The median lethal concentrations (LC50) were determined to be 7.1 × 104 cfu/mL, 3.3 × 103 conidia/mL, 1.2 × 103 PIB/mL, and 1 × 103 PIB/mL, respectively. These results indicate that indigenous microbial agents are a promising environmentally friendly alternative to chemical pesticides in the control of H. derogata.

This study was undertaken to investigate the molecular detection of biofilm-producing Staphylococcus aureus (S. aureus) from goat mastitis. An overall 384 milk samples were initially screened for subclinical mastitis (SCM) followed by molecular characterization of S. aureus isolates. The biofilm formation was assessed using Congo Red agar (CRA), a microtiter plate and the presence of the icaA gene. The results revealed a molecular prevalence of 53.24% (115/216) for pathogenic S. aureus in milk samples of goats. The phenotypic prevalence of biofilm production by CRA and microtitre methods was recorded to be 38.26% (44/115) and 26.96% (31/115) respectively, while the molecularly confirmed biofilm-forming S. aureus through polymerase chain reaction targeting icaA gene was 58.26% (67/115). The phylogenetic analysis of icaA gene revealed high identity between sequences of study isolates and the isolates of other neighbouring countries. The antibiogram profiling of pathogenic S. aureus showed increased resistance to cefoxitin and oxytetracycline followed by gentamicin. Out of 115, 45.22% (52/115) were declared as multiple drug resistant with multiple antibiotic resistance index greater than 0.2. The study concluded that biofilm-producing S. aureus strains are considered to be a common cause of SCM in dairy goats of Pakistan and biofilm formation is associated with multidrug resistance of study isolates.

With the increasing number and diversity of reptile species kept in zoological facilities and households, their welfare in captivity warrants structured and consistent evaluation. However, focused research on reptile welfare remains limited within the broader field of animal welfare science. Recognising such a gap, this study adopts an evidence-informed approach to review existing literature and proposes two conceptual welfare assessment frameworks — one for zoo settings and another for private keeping. We first identify the intended audiences for each framework and discuss common challenges reptile caretakers may face when conducting welfare assessments in different contexts. The frameworks are grounded in established principles from the Five Domains model and the European Welfare Quality® protocol, incorporating both resource- and animal-based indicators under the domains of Environment, Nutrition, Physical Health, and Behaviour. The design rationale is also explained to support future refinement. Finally, these conceptual frameworks are intended as a foundation for the development and validation of adaptable tools, capable of guiding improved husbandry practices and resource allocation for better welfare outcomes across a broad range of reptile taxa.

Dietary patterns are prerequisites for health and integral components of ecological systems. For over a century researchers have been building a body of evidence of associations between dietary patterns and health and sustainability outcomes while policymakers have been synthesising and translating this evidence into policies to promote public health. During this period, food systems have dramatically changed and driven the emergence of food supplies and dietary behaviours with no ecological or evolutionary precedent. Now, the relevance of conventional nutrition research and policymaking approaches for understanding food system transitions and protecting against unhealthy and unsustainable diets is being questioned. This review aims to examine how the ecological nutrition paradigm might guide a transformed approach to nutrition research and policymaking to promote healthy and sustainable diets. It shows the ecological nutrition paradigm is transdisciplinary integrating biological, social and environmental dimensions into nutrition research and policymaking. The paradigm operates to a ‘fit-for-purpose’ policymaking orientation. It draws on ecological and evolutionary theories to provide insights to conceptualise the causes of, and solutions to, nutrition problems and help design relevant decision-making processes. These research and policymaking features contrast with the ‘one-size-fits-all’ policymaking orientation and prescriptive decision-making processes of the conventional medical nutrition paradigm. Their attention to a relevance criterion engenders confidence in the likely effectiveness, and ability to avoid unintended consequences, of policies informed within an ecological nutrition paradigm. The review proposes a shift to the ecological nutrition paradigm to transform nutrition research and policymaking for promoting healthy and sustainable diets is overdue.

The objective of this experiment was to evaluate the resistance of advanced tomato genotypes resulting from an interspecific cross between Solanum lycopersicum and Solanum pennellii to Tetranychus urticae and Phthorimaea absoluta. The plant materials included nine lines, 14 hybrids, Leblon F1 (commercial control), and the wild accession S. pennellii LA716 as a resistance standard. Acylsugar content was then determined. For mite bioassays, oviposition, adult mortality/survival, egg hatching, and nymphs were evaluated using a no-choice bioassay. For P. absoluta bioassays, the oviposition, intensity of damage, type of lesions, and percentage of damaged leaflets were evaluated. F1 (TOM-808 × BPX-443E-03-02-113-02), F1(TOM-810 × BPX-443E-03-02-113-02), F1(TOM-808 × TOM-717), F1(TOM-808 × TOM-757), and F1(TOM-810 × TOM-717) were the most resistant to the mite, exhibiting higher female mortality, reduced oviposition, and no nymph emergence observed. The genotypes F1(TOM-808 × TOM-667), F1(TOM-808 × TOM-717), F1(TOM-810 × TOM-615), and several lines, which exhibited reduced oviposition and foliar damage. The results of the bioassays indicated that high acylsugar content reduced oviposition and foliar damage of the tested pests. The hybrid F1(TOM-808 × TOM-717) is the most promising at this stage of the breeding program, as it shows resistance to both tested pests.

The European red spider mite, Panonychus ulmi Koch (Acari: Tetranychidae), is a major mite pest affecting a wide range of crops globally. Its rapid development and extensive dispersal enable P. ulmi to form large colonies through arrhenotokous reproduction, which subsequently produces bisexual offspring following oedipal phase mating. In this study, life tables for arrhenotokous and bisexual P. ulmi populations were constructed and assessed using the age-stage two-sex life table theory. To assess whether maternal age affects the offspring sex ratio, 3D life table analysis was conducted for both groups. Population projection for P. ulmi bisexual and arrhenotokous was based on life table data to evaluate the effect of arrhenotokous reproduction on population growth. Bisexual population parameters were r = 0.1828 d−1, λ = 1.2006 d−1, R0 = 44.80 offspring, and T = 20.79 d. The theoretical calculation of these parameters cannot be performed solely based on the survival rate and female fecundity of the P. ulmi arrhenotokous population, as only male offspring were produced during the first seven days before the arrival of the bisexual population. Although arrhenotokous parthenogenesis yields fewer offspring than bisexual reproduction, it significantly influences population dynamics by enabling a single virgin female to establish a bisexual population. Controlling offspring sex ratios enables rapid population growth and colonisation of new habitats. A comprehensive understanding of arrhenotokous reproduction is crucial for developing effective management strategies for P. ulmi. Future research should integrate genomic, ecological, and evolutionary perspectives to bridge the gap between laboratory and field conditions.

In discussions on European Neogene continental chronology, the Kastellios Hill section has played an important role because of the presence of strata with planktonic foraminifers and strata with mammalian remains. With the primary papers written in the 1970s and 1980s, the time is ripe for an update on age and taxonomy of the murid rodents from Kastellios Hill by comparing the fauna with time-equivalent southern and central European faunas. This comparison results in a partly revised faunal list consisting of the dominant Progonomys mixtus n. sp., the less common Cricetulodon cf. C. hartenbergeri Freudenthal, 1967 and P. cathalai Schaub, 1938, and the rare P. hispanicus Michaux, 1971 and cf. Hansdebruijnia neutra (de Bruijn, 1976). Based on the updated species list and magnetic polarity data, the most probable age of the Kastellios Hill mammal localities is 9.3–9.1 Ma (Chron C4Ar.1r, late Vallesian, MN10). The genus Hansdebruijnia is narrowed down to two species in an ancestor–descendant relationship: the ancestral type species H. neutra, which is restricted to southeastern Europe and Anatolia, and the descendant species H. magna (Sen, 1977), representing a new combination and including ‘Occitanomys alcalai’ Adrover et al., 1988 and ‘O. debruijni’ (Hordijk and de Bruijn, 2009). H. magna colonized both southeastern and southwestern Europe.

UUID: http://zoobank.org/ebe0f64c-6900-4efc-ab43-8a4045de6810

We report the first identification of nematode parasitism in a nemertean host (Arctostemma arcticum), representing a novel host-parasite interaction in marine ecosystems. Larvae of the anisakid nematode were discovered within the rhynchocoel of A. arcticum collected from the White Sea, suggesting transmission via ingestion of infected crustaceans – a previously undocumented pathway. The nematode was identified as Phocanema bulbosum through both morphological and molecular analyses.

The presence of nematode in nemertean A. arcticum implies trophic transmission through nemertean predation on infected amphipods, supported by hoplonemertean feeding strategy. While some fish occasionally consume nemerteans, their low predation frequency suggests A. arcticum could act as a dead-end host.

This study expands the known host range of Anisakidae, though it remains uncertain whether nemerteans serve as competent hosts in the P. bulbosum life cycle. Nevertheless, the findings highlight the need to assess nemerteans’ potential influence on parasite dynamics in fisheries and aquaculture, particularly where they coexist with intermediate and definitive hosts.

Seabirds are experiencing a decline in their populations because of climate change and human activities. Understanding their spatiotemporal dynamics is crucial for effective conservation, but the distribution and movement patterns of pelagic seabirds are not yet fully elucidated. In the present study, we investigated the seasonal movements and wintering areas of Swinhoe’s Storm-petrel Hydrobates monorhis, a Near Threatened species that breeds primarily on islands in the north-western Pacific. The data analyses of geolocators retrieved from four birds showed that Swinhoe’s Storm-petrels migrated across the north-western Pacific and Indian Oceans and wintered in the Arabian Sea. The distance between their breeding colony and the wintering area was approximately 6,700 km, and the tracking distance for a seasonal migration exceeded 12,000 km. The migration pathway was characterised by large-scale movements in both north–south and east–west directions in the Eurasian offshore regions, which previously had been inferred from direct observations at such areas but not empirically confirmed. Wintering areas in the Arabian Sea overlapped with regions where plankton blooms are triggered by the monsoon in winter, which may produce high marine productivity and support the wintering of Swinhoe’s Storm-petrels in this sea area.

The Southern Ocean, a region characterized by high nutrient levels but often low productivity, hosts dynamic picophytoplankton communities crucial for its food web. This study investigated the spatial and inter-annual variability of picophytoplankton abundances and their environmental drivers in the Indian sector of the Southern Ocean during the austral summers of 2018 and 2020. Using flow cytometry for picophytoplankton quantification and standard oceanographic methods for environmental parameters (temperature, salinity, nitrate, phosphate, silicate), we employed descriptive statistics, inferential group comparisons (t-tests, analysis of variance), principal component analysis (PCA) and principal component regression (PCR) to analyse the dataset. Our analyses revealed significant differences in picophytoplankton abundances and environmental conditions across distinct oceanic fronts, between deep chlorophyll maximum and surface depths and, notably, between the two study years. PCA identified three major environmental gradients explaining over 93.5% of the variance in temperature, salinity, nitrate, phosphate and silicate. PCR confirmed our hypothesis: the abundance and carbon biomass of picoeukaryote II (PEUK-II) picophytoplankton was statistically significant overall (F-statistic = 3.415, P = 0.0290). The model explained 24.2% of the variance in PEUK-II abundance (R2 = 0.242), indicating its sensitivity to dynamic oceanographic conditions, with PC3 (primarily representing a salinity gradient) being a significant predictor. Conversely, Prochlorococcus-like/Synechococcus picophytoplankton abundance was not statistically significant overall (F-statistic = 2.068, P = 0.124), suggesting control by other, potentially non-linear factors. These findings highlight distinct ecological strategies among picophytoplankton groups and are vital for predicting their roles in the Southern Ocean’s microbial food web amidst ongoing environmental change.

Rice cultivated under furrow irrigation faces weed management challenges due to the aerobic conditions favoring terrestrial weed emergence, like Palmer amaranth. Fluridone is an HRAC/WSSA Group 12 herbicide recently registered for use in rice, offering an alternative site of action for Palmer amaranth control. Four site-years of field experiments were conducted in 2022 and 2023 in furrow-irrigated rice to assess Palmer amaranth control and crop tolerance to fluridone applied preemergence (PRE) alone or with different postemergence programs. The experiment was a randomized complete block design with a split-plot arrangement and four replications. The whole-plot factor was the postemergence program, while the subplot factor was fluridone applied PRE at 0, 84, 168 (1× labeled rate), and 336 g ai ha−1. Postemergence programs included no herbicide, a single florpyrauxifen-benzyl application at 6 wk after rice emergence (WAE), and a weed-free control. The 2× rate of fluridone caused the greatest visual injury compared to the 0.5× rate across site-years at two and five (WAE), ranging from 8% to 34%. The 1× and 2× rates of fluridone provided the greatest Palmer amaranth density reduction four wk after treatment (WAT). However, the effect diminished or became less prominent by eight WAT, reducing control across fluridone treatments. The follow-up application of florpyrauxifen-benzyl reduced Palmer amaranth density at rice harvest in most instances and diminished seed production by ≥ 94% compared to its absence. Fluridone application, regardless of the rate, did not affect rough rice grain yield under weed-free conditions. These findings suggest that integrating fluridone with a subsequent florpyrauxifen-benzyl application enhances Palmer amaranth management in furrow-irrigated rice compared to fluridone alone. However, sequential applications are needed for successful Palmer amaranth control.