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Centromeres are chromosomal loci essential for the correct segregation of genetic material during cell division. Defects in centromere function can lead to aneuploidy and cancer. During early embryonic development in mammals, prior to the first cell division, male and female genomes are separated in pronuclei located at the centre of the zygote. Parental chromatin clusters at the interface between the two pronuclei and this clustering step is critical to avoid aneuploidy in human and bovine zygotes. Yet, despite their essential function in chromosome segregation, the position and spatial organization of centromeres during the first cell cycle in mammals is mostly unknown. Previous studies conducted in bovine embryos derived from in vitro fertilization (IVF) showed that cell cycle progression impacts on the success rate of blastocyst formation. Specifically, embryos that entered earliest into S-phase or the earliest cleaving embryos were more likely to develop into blastocysts. To determine the precise timing of these events we performed a detailed characterization of key phases of the first cell cycle in bovine zygotes derived from IVF. In parallel we examined the spatial positioning of centromeres. We identify 20 h post insemination (hpi) as the timepoint when male and female pronuclei are juxtaposed and are completing S-phase. At this timepoint, we show that centromeres are positioned distal to the pronuclear interface and use super resolution microscopy to demonstrate extensive centromere clustering into chromocentres. Our results identify distinct nuclear features observed at 20 hpi, which may serve as cell cycle markers in determining successful bovine IVF.
Strongyloides stercoralis infection affects approximately 600 million individuals worldwide. This parasite has the ability to exacerbate infection through internal autoinfection, which can lead to hyperinfection and/or dissemination, conditions associated with high morbidity and mortality, particularly in immunocompromised patients such as those with alcohol use disorder (AUD). In this study, we conducted a meta-analysis to assess the prevalence and risk of having S. stercoralis infection among individuals with AUD. Searches were performed in the PubMed, Embase, and LILACS databases to identify studies investigating the prevalence of S. stercoralis infection in individuals with AUD, with or without comparison to non-alcoholic groups. The pooled prevalence was calculated using the Probit Logit (PLOGIT) transformation, and the odds ratio (OR) was used for risk comparison. The initial search yielded 154 studies, of which seven were included in the systematic review and meta-analysis. The combined prevalence of S. stercoralis infection among patients with AUD was 16.9%. Risk analysis based on four studies showed that individuals with AUD had a 6.08-fold higher risk of infection compared with non-alcoholic individuals. These findings highlight chronic alcoholism as a significant risk factor for strongyloidiasis, likely due to a combination of environmental, physiological, and immunological factors. This meta-analysis underscores the critical need for routine screening for S. stercoralis infection in patients with AUD, even in the absence of clinical symptoms, to ensure early detection and timely intervention.
To assess anthropometric failure prevalence using a composite index of anthropometric failure and evaluate the concordance of mid-upper arm circumference (MUAC) with weight for length z score (WLZ) in identifying acute malnutrition in children.
Design:
We used data from a pre-proof-of-concept (pre-POC) study conducted in 2022–2023.
Setting:
The study was conducted in the Vellore district in Tamil Nadu, South India.
Participants:
We included all children aged 5–19 months who were pre-screened for the pre-POC study and had available data on weight, length, MUAC, date of birth and child sex.
Results:
A total of 663 children were available for analysis, with a mean age of 11·4 months. The prevalence of underweight, stunting and wasting was 23·8 %, 24·3 % and 16·6 %, respectively. 36·7 % of the children had at least one form of anthropometric deficit, and 8·2 % showed severe deficits. We found a fair agreement between MUAC and WLZ values (kappa = 0·32) and a poor agreement of kappa = 0·19 and 0·10 with weight for age z score (WAZ) and length for age z score (LAZ), respectively.
Conclusions:
The prevalence of malnutrition remains a significant public health concern in South India, with rates consistent with previous literature. MUAC and WLZ cutoffs by the WHO identify different groups with acute malnutrition. Community-based studies using WLZ criteria can adopt screening with higher MUAC cutoffs, followed by WLZ or, alternatively, WAZ alone, as low WAZ covers most participants with low MUAC and WLZ, providing better feasibility. However, the optimal measurement for risk identification requires further exploration.
A pair of dizygotic (DZ) twins discordant for Turner syndrome are discussed with reference to the biological origins of the condition and the effects of discordance on the twin relationship. There is little research on how having an atypical twin influences the life events and goals of the typical twin. Next, timely reviews of research on preventing premature twin birth, a twin gestation with hydatidiform mole, an update on Feingold syndrome twins discussed in a previous issue of this journal, and qualitative monozygotic twin difference studies are presented. The final portion of this article covers human interest stories of twins that are variously entertaining and enlightening. They include identical twins who celebrated their 100th birthday together, twins in famous families, celebration of the Yorùbá twins of Nigeria, identical artistic partners, and surgical separation of a rare, conjoined twin set.
Despite growing front-of-pack labelling (FOPL) policy implementation in low-and middle-income countries (LMIC), research approaches for evaluating these policies remain poorly characterized, hindering evidence-based policy development and methodological gaps. This study explored research approaches, frameworks, and methods used in assessing FOPL policy implementation and response in LMIC.
Design:
Systematic search of five databases, including Medline, Web of Science, Scopus, Global Health, and CINAHL, for peer-reviewed articles published between 2014–2025. Studies on FOPL policy implementation or response in LMIC were included. Data on study characteristics, methods, and findings were extracted and synthesized.
Setting:
LMIC.
Participants:
All populations.
Results:
Thirty-one studies revealed significant research imbalances. Implementation studies (n 3) used qualitative approaches with policy theories, while response studies (n 28) predominantly employed quantitative methods including surveys, experiments, and modeling. Pronounced geographical bias emerged, with 24 studies conducted in Latin America while other LMIC regions remained underrepresented. Common limitations included non-representative sampling, self-reported data, and short timeframes. Mandatory FOPL policies achieved higher compliance than voluntary schemes, though implementation faced challenges including inadequate monitoring, limited resources, and industry resistance. Consumer awareness was generally high but varied significantly across population groups, revealing substantial equity gaps.
Conclusions:
This review reveals critical gaps in FOPL implementation research in LMIC, with evidence heavily skewed toward consumer responses and geographically concentrated in Latin America. Future research should prioritize implementation science approaches, geographical diversity, and understanding policy processes in resource-constrained settings to develop effective, context-appropriate FOPL policies.
The lactation curve expresses the pattern of milk production throughout the lactation period. Such a curve provides insights to assist in designing proper management strategies. Culling dairy cows directly influences the farm economy and animal welfare. The lactation curve components (LCC) of culled Holstein cows, compared with those of retained cows, have not been studied. This study aims to investigate the LCC in culled Holstein cows compared with those retained unculled in the same herd. This research included 27,297 complete lactation records for Holstein cows described as retained or culled for low milk yield, reproductive disorders, udder problems, metabolic disorders, locomotive problems, endemic diseases, respiratory diseases and unknown reasons. The incomplete gamma function was fitted to estimate LCC, as represented by initial milk yield (IMY), the rate of milk increase to peak, the rate of milk decline after peak, peak yield (PY), time to reach peak and persistency. The general linear model was applied to analyse the effects of stayability class (retained/culled) on LCC. Cows culled for reproductive disorders showed no significant differences in LCC compared to retained cows, but they spent more days in milk (54.9 weeks) than retained cows (48.9 weeks). Except for those with reproductive disorders, all culled cows exhibited shorter lactation lengths, higher rates of milk decline after peak, shorter times to attain PY and lower persistence than retained cows. In addition, cows culled for metabolic disorders exhibited higher initial milk (35 kg) and peak milk yields (44.2 kg) than the retained cows and those culled for other reasons. In conclusion, by linking culling causes to milk production trends, this research equips farmers to identify risks earlier, such as tracking milk decline onset and adapting management to retain healthier, high-value cows longer. This strategy could reduce costs, enhance milk output and improve herd welfare on dairy farms.
This chapter delves into the various physiological measures that can be used to gauge the thriving status of animals, emphasising the importance of routine veterinary examinations, body condition scoring, and quality of life assessments. It discusses the integration of these measures into a comprehensive animal welfare strategy, ensuring that each animal receives the necessary care tailored to its specific physiological needs. The chapter also highlights the implications of these practices for improving animal welfare, advocating for a science-based approach to animal care that aligns with the latest research and ethical standards in the field. This abstract provides a concise overview of the chapter’s focus on the critical role of physiological health in the overall welfare of animals, underscoring the necessity for ongoing research and adaptive care practices to enhance the quality of life for animals in human care.
Soybean aphids (Aphis glycines) (Hemiptera: Aphididae) pose a serious threat to global soybean production, necessitating sustainable control strategies. This study investigated silica nanoparticles (SiNPs) as an eco-friendly alternative, hypothesising they would suppress aphid populations while enhancing plant growth. Soybean plants were foliar-sprayed with SiNPs (0–1 mmol/L), and aphids were assessed across six assays: fecundity, survival, feeding preference, weight gain, olfactory response, and plant morphometrics. SiNPs significantly reduced aphid nymphal production and population growth at all concentrations but did not affect survival, weight gain, or host-seeking behaviour. Plant responses were mixed: leaf width increased at higher SiNPs doses, but plant height decreased, with no effects on leaf length, root/shoot biomass, or root length. These findings suggest that SiNPs could disrupt aphid reproduction without triggering behavioural avoidance. The absence of biomass reduction indicates potential for crop compatibility. This laboratory study reveals a novel, reproduction-targeted mode of action for SiNPs, highlighting its potential as a candidate for future development in sustainable IPM strategies. Further field-scale validation is required to confirm these effects under real-world conditions.
There exists a multifaceted concept of thriving as opposed to mere survival in animal care. The chapter begins by establishing a foundational understanding of what it means for animals to truly thrive, emphasising that thriving encompasses not only physical health but also psychological, emotional, and social well-being.
The chapter further explores the various aspects of thriving. It discusses physical thriving through adequate nutrition and exercise, psychological thriving through mental stimulation and problem-solving activities, emotional thriving via positive interactions and stable environments, and social thriving through healthy relationships within species groups.
Additionally, the chapter introduces Vicino and Miller’s Five Opportunities to Thrive, which provide a structured approach to evaluating and enhancing animal welfare. These opportunities include ensuring safety, facilitating natural behaviours, promoting positive states, preventing negative states, and fostering growth and development. This framework serves as a guide for care technicians to create environments that support all dimensions of animal welfare.
Overall, this chapter sets the stage for a deeper understanding of animal welfare, advocating for a holistic approach to animal care that goes beyond basic needs to ensure animals lead fulfilling lives.
There is a critical role that environmental factors play in the thriving of animals. This chapter emphasises the distinction between natural and artificial environments and their respective impacts on animal welfare. It discusses the importance of habitat, the degree of physical movement possible, cognitive stimulation, social dynamics, and the intrinsic rewards that environments can provide. Further, it addresses the challenges and compensations associated with artificial environments. The narrative includes discussions on the limitations imposed by artificial settings and the innovative ways these can be mitigated to simulate natural habitats as closely as possible. The chapter concludes by stressing the importance of designing environments that cater to the specific needs of different animal species, thereby ensuring their psychological and physical thriving. The implications of these environmental factors are profound, suggesting that the well-being of animals is heavily influenced by the quality and design of their surroundings.
This chapter serves as a metaphor for the meticulous and informed approach required in animal curation, emphasising that both disciplines are sciences that benefit from empirical knowledge and careful planning.
Thriving is uniquely manifested across different animal species. This chapter emphasises that each species has evolved distinct physiological and psychological mechanisms tailored to their specific environmental interactions, which are crucial for their ability to thrive.
The chapter outlines how these species-specific traits dictate the natural behaviours and cognitive functions of animals, highlighting the importance of understanding these unique characteristics to ensure proper care and welfare in captivity. It discusses the challenges and barriers each species may face in artificial environments and how these can be mitigated to support their well-being.
Furthermore, the chapter explores the concept of ‘thriving’ not just as survival but as a holistic state where an animal’s physiological, psychological, and ecological needs are fully met. This includes the ability to exhibit natural behaviours and interact with the environment in a way that promotes their overall health and vitality.
By providing a detailed analysis of species-specific thriving, this chapter serves as a crucial foundation for developing effective animal welfare practices that are tailored to the unique needs of each species, ensuring that they not only survive but truly thrive in human care.
This study aimed to assess the prevalence of non-sugar sweeteners (NSS) in Brazilian food products before the implementation of new nutritional labeling legislation. Specifically, we aimed to determine the eligibility of these products to contain NSS according to RDC no. 18/2008, which governed the use of NSS in Brazil during the study period.
Design:
Data were collected from 3335 packaged foods and beverages available in one of Brazil’s top 10 supermarket chains, six months following the publication of front-of-package nutrition labeling (FoPNL) and 19 months before the legislation came into force.
Setting:
The study was conducted in Brazil.
Results:
Our analysis revealed that NSS were present in 12.5% of the sampled products. Notably, high frequencies of NSS were observed in powder dessert mixes and soy drinks (100%), gelatin preparations (88.1%), chewing gum (87.1%), tea (84.6%), and carbonated beverages (71.4%). Furthermore, we found that 82% of products containing NSS made claims regarding sugar and calorie reduction, with 16.6% of these claims being inconspicuous. Additionally, 14% of products targeted controlled sugar intake diets, 0.5% aimed at sugar-restricted diets, and 4% were ineligible for NSS use. Importantly, the declared NSS content adhered to Brazilian regulatory limits.
Conclusions:
While most products complied with regulatory standards, our findings highlight the presence of ineligible products and less prominent claims, which may complicate NSS identification for consumers. Continuous monitoring of NSS prevalence, especially following the implementation of FoPNL, is essential for ensuring compliance with regulations and promoting informed consumer choices in Brazil.
This chapter highlights the often overlooked or underestimated obstacles that can impede the thriving of animals under human care. This chapter critically examines the internal and external factors that contribute to these pitfalls, providing a nuanced understanding of the multifaceted nature of animal welfare. Key themes include the identification of species-specific and individually specific challenges, the impact of environmental and historical variables, and the strategies for mitigating these issues to enhance animal welfare. The chapter also discusses the importance of comprehensive behavioural and physiological assessments in identifying and overcoming these barriers, emphasising a holistic approach to animal care that goes beyond basic survival to ensure psychological, emotional, and social thriving.
Developed by B. F. Skinner, a prominent Harvard psychologist, operant conditioning distinguishes itself from classical conditioning by focusing on learned behaviours rather than reflexive responses. Skinner’s approach emphasises the impact of external environmental factors over internal psychological states in shaping behaviour. This perspective has been particularly influential in fields such as organisational management, where behaviour modification is often applied to improve workplace dynamics. The chapter further explores how operant conditioning can be utilised in management practices by using reinforcements such as pay raises and promotions to motivate employees. This method does not rely on the internal states like beliefs or motivations but rather on modifying the external environment to achieve desired behavioural outcomes. Overall, this chapter not only delves into the historical and theoretical foundations of operant conditioning but also discusses its practical applications in various settings, providing a thorough understanding of both its principles and utility.
There are complexities that hinder the thriving of animals in both natural and artificial environments. This chapter provides a detailed examination of the obstacles animals face and the strategies employed to mitigate these challenges. Barriers to thriving can be classified into two main types: environmental challenges (the multiplier) and internal challenges (the multiplicand). Environmental challenges include adapting to or changing an environment, while internal challenges focus on species-specific and individually specific causes. Significant emphasis is placed on the concept of the ‘Sordid Six’, which represents key environmental obstacles that animals face in captivity. The chapter also discusses mitigation strategies, termed as the divisor, which are essential for reducing the impact of these barriers and enhancing animal welfare.
Animal curation is a vital and evolving discipline that integrates science, policy, and hands-on care to ensure the highest standards of animal welfare. As the role of zoos, aquaria, sanctuaries, and research facilities expands beyond exhibition to conservation and education, the management of animals under human care has become increasingly scientific. This book provides a comprehensive guide to the organisation, policies, and procedures essential for effective animal care programmes. It emphasises evidence-based practices in husbandry, veterinary care, and facility management while prioritising both animal well-being and staff safety. Through detailed chapters and real-world case studies, readers will explore species-specific needs, ethical considerations, and regulatory compliance. Designed for students and professionals in animal science, welfare, and conservation, this book moves beyond basic care, focusing on the concept of ‘thriving’ rather than mere survival. It is an essential resource for shaping the future of animal management and welfare.