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Avian Plasmodium parasites can be pathogenic to their vertebrate hosts. Although cases of anaemia are frequently reported in parasitized birds, the potential damage caused by the parasite during the exoerythrocytic reproduction phase remains poorly investigated. Here, we report 2 individuals of red-legged seriemas (Cariama cristata) infected with 2 different lineages of Plasmodium huffi, one of them exhibiting potential malarial-compatible tissue lesions in the spleen, liver, brain and lungs, alongside molecular confirmation of parasite presence in the spleen. Previously classified as specific to birds from the order Piciformes, this parasite has shown different associated lineages amplified across diverse host orders in South America (Anseriformes, Charadriiformes, Columbiformes, Galliformes, Pelecaniformes and Passeriformes). Those infections, however, were defined as abortive due to the absence of gametocytes visualized in blood smear slides. Herein, we confirm P. huffi as a generalist parasite based on the first morphological characterization in the peripheral blood of a bird outside the Piciformes order. This is also the first morphological and molecular description of a Plasmodium species in Cariamiformes. In addition to the morphological analyses, we have also proposed a novel phylogenetic hypothesis based on the partial cytb gene and the near-complete mitochondrial genome of this parasite. Our findings support that the division of the genus Plasmodium into subgenera is not monophyletic, as P. (Huffia) huffi and its associated lineages cluster more closely with Plasmodium (Haemamoeba) gallinaceum than with Plasmodium (Huffia) elongatum.
The chronology of the Bronze Age in the Carpathian basin is largely based on relative chronologies, i.e. stylistic analysis of ceramic (and other) materials. While the number of radiocarbon dates is generally increasing, certain important sites are still poorly dated. One of the largest necropolises from this period, i.e. Mokrin necropolis, which traditionally belongs to Maros culture, is dated only with 6 radiocarbon dates. Here we synthesize the previous 6 radiocarbon dates with 13 new radiocarbon dates, with two goals in mind: 1) to explore the absolute chronology of the site, specifically to determine its chronological limits; and 2) to test hypotheses about the spatio-temporal organization of the site. Our data show that the chronological limits of the necropolis were most probably between 2073 and 1822 BC. Concerning traditional relative chronologies, none of the previous hypotheses about the internal chronological development of the necropolis is supported by data. Our results suggest that all parts of the necropolis were used relatively simultaneously.
Rapid advances in species distribution modelling have been facilitated by open availability of ‘big data’ and powerful statistical methods. A key consideration remains the time window over which field recorded occurrence data are sampled to develop a baseline species distribution. Too narrow, and distributions are incomplete and affected by sampling bias, too broad and distributions may fail to meet an assumption of equilibrium, having been affected by dynamic change across a range of different predictors. Lichens are a case in point; being diverse, functionally important and the subject of bioclimatic modelling for conservation assessment, they are nevertheless a specialist taxonomic group that is comparatively less well recorded compared to birds, mammals or vascular plants, for example. In this study, we examined the distribution of the ‘hair-lichen’ Bryoria fuscescens, based on UK record data. We partitioned records into sub-decadal periods (1970s, 1990s, 2010s), and accounting for recording effort, we compared these distributions to three predictors: an historical reconstruction of two different pollutants (sulphur dioxide and nitrogen deposition), and the climate (minimum mean temperature). We asked whether the strength of evidence for the effect of environmental predictors on Bryoria fuscescens distribution varied among the different decades, while also considering a potential for lag-effects. We show that a Bryoria fuscescens distribution that appears static, is dynamic when referenced against patterns of field recording effort. Climate was consistently important in explaining Bryoria fuscescens distribution, which was also affected by the changing pattern of pollution over time. This included a lag-effect of peak sulphur dioxide in the 1970s, and accrued effects of nitrogen deposition that strengthen over time. Overall, we conclude that Bryoria fuscescens has undergone a long-term decline in extent over the last six decades, caused by complex multivariate effects of air pollution, probably combined with climate warming. The ability to resolve these trends for assessment against future conservation targets depends critically on maintaining field identification skills and a sufficiently robust recording effort.
Financial institutions spanning Global North and South are increasingly adopting an agenda of ‘women’s financial inclusion’. The women’s inclusion agenda in finance reflects dynamics of deep marketization that prescribe common economic policy solutions, transcending formerly significant distinctions of geography and social context. In this case, the closing of gender gaps is the universally proscribed policy. Yet this agenda elicits vastly different practices in ‘high’ finance registers where women are recruited as professionals, and microfinance registers where women are incorporated as borrowers. Relating multisited ethnographic materials from a US gender diversity organization and microfinance institutions in India, we ask: on what terms does inclusion take place? First, we examine how gender is constructed across finance institutions by essentializing women as virtuous. These constructions play out according to context-specific gender politics on questions of women’s economic empowerment – concerning neoliberal iterations of feminism in the US case, and financialization of social reproduction in India. Second, what do women’s everyday engagements with the inclusion agenda indicate about the terms of financial inclusion? Women contend with characterizations of themselves as risk-averse professionals and responsible borrowers, respectively, with ambivalence. Their contextually located ambivalent responses are points of both leverage and critique for the financial inclusion agenda.
This article investigates the developments of hawker discourse and movements across the Malay(si)an peninsula in the first decade of independence. Looking at news coverage and municipal records, it examines the contingent, gendered, and egalitarian qualities of hawking as labour which led to its adoption by people experiencing hardship, and influenced the ways in which municipal authorities and the public discussed hawkers. In effect, hawkers, long significant to the historical and cultural systems of Malayan trade, were recharacterized as vulnerable subjects at the urban margins. The article then explores how local administrations understood and regulated hawkers through categories of location, race, and food, shaping the politics and governance of hawkers in public spaces. To engage with such governance, hawkers formed associations that protested against injustice and established dialogue with municipal and town councils, impelling authorities to consider a more significant inclusion of hawking in street planning. Throughout the period, the potential and limits of hawker inclusion in post-colonial public spaces became subject to significant debate between municipal authorities, political representatives, and hawkers. As local administrations eventually deepened their commitment to support hawkers, they also expanded their regulation, signifying a cautious imperative to legitimate hawkers and influencing the logic of post-independence planned spaces.
Thrust changes near walls and the ground plane are influenced by the rotor’s position in indoor flight environments. This study evaluates variations in rotor thrust near a corner, which includes one wall and the ground plane, as well as a vertex, which involves two walls and the ground plane; these phenomena are referred to as the corner and vertex effects, respectively. Additionally, the rotor wake in the vertex effect was visualised using the laser sheet method, and wake velocity was measured with a hot-wire probe. The thrust change in the corner effect on the ground side was minimal, primarily depending on the ground effect. In the vertex effect, thrust decreased to 93% of the thrust outside the vertex effect when the rotor height above ground was 2.5 times the rotor radius, and the rotor was distanced from the two walls by 1.5 times the rotor radius. Flow visualisation and hot-wire velocimetry results suggest that the thrust decrease was caused by the flow recirculation structure between the fountain flow developed along the vertical corner and rotor inflow. The thrust decreases under conditions where the circulation structure appears, and fountain flow velocity accelerates the recirculation. These findings aid in planning the flight path of small multirotors in indoor flight conditions by providing guidance on distances that do not alter rotor thrust near corners and vertices.
What is ontological (in)security? Recent scholarship on ontological security in International Relations has increasingly turned to the concept's theoretical origins in psychoanalysis and existential philosophy to address the field's (meta)theoretical limitations. This article argues that this development also necessitates an interrogation of the concept of ontological security itself to address the field's theoretical tensions. Further developing the nascent Kleinian approach to ontological security, this article conceptualises ontological (in)security as two distinct positions that denote the different ways in which subjects, be they individuals, groups, or states, manage anxiety. To develop this proposition, the article draws on Melanie Klein's work on the paranoid-schizoid and depressive positions to elucidate these positions of ontological (in)security, their respective defence mechanisms against anxiety, and their socio-political implications. This Kleinian approach facilitates a clear theoretical distinction between security and insecurity, providing an analytical toolbox to differentiate the various ways in which anxiety is managed in different positions. This framework particularly underscores the ethical, reparative, and transformative potential of the position of ontological security, aspects that have received limited theoretical and empirical attention to date.
Developmental regression in children, in the absence of neurological damage or trauma, presents a significant diagnostic challenge. The complexity is further compounded when it is associated with psychotic symptoms.
Method
We discuss a case series of ten children aged 6–10 years, with neurotypical development, presenting with late-onset developmental regression (>6 years of age), their clinical course and outcome at 1 year. A comprehensive clinical evaluation, laboratory investigations and neuroimaging ruled out any identifiable neurological cause.
Results
Mean age at regression was 7.65 (s.d. 1.5) years and mean illness duration was 10.1 (s.d. 8.5) months. The symptom domains included regression (in more than two domains – cognitive, socio-emotional, language, bowel and bladder incontinence), emotional disturbances, and hallucinatory and repetitive behaviours. Response to treatment was gradual over 6 months to 1 year. At 1-year follow-up, nine children did not attain pre-regression functioning, and residual symptoms included not attaining age-appropriate speech and language, socio-emotional reciprocity and cognitive abilities.
Conclusions
These cases demonstrate a unique pattern of regression with psychiatric manifestations, distinct from autism spectrum disorder and childhood-onset schizophrenia. The diagnostic dilemma arises from the overlap of symptoms with childhood disintegrative disorder (CDD), childhood-onset schizophrenia and autism. This study underscores the diagnostic intricacies of this clinical presentation and highlights the need for longitudinal follow-up to unravel the transitions in phenomenology, course and outcome. For severe manifestations such as developmental regression, where the illness is still evolving, considering CDD as a non-aetiological and transitory/tentative diagnosis would aid against premature diagnostic categorisation and provide scope for ongoing aetiological search.
This study analyzed the practice of switching intravenous antibiotics to oral dosage form in a tertiary care hospital of Nepal.
Design:
A prospective observational study was performed among patients admitted to medical/surgical wards in a private tertiary care hospital of Nepal.
Methods:
Hospitalized adult patients who received IV antibiotics for at least 48 hours and met the eligibility criteria were enrolled in the study. The detailed information on use of antibiotics such as indication, duration, type time of switch etc. were collected and analyzed.
Results:
Among 335 patients, 282(83.9%) met the eligibility criteria for intravenous (IV) to oral conversion however, only 18.7% of patients were switched to oral regimen. Step-down conversion was the common type of IV to oral switch. Almost all patients were empirically treated with β-lactams antibiotics (n = 327). There was significant association between the antibiotic class and duration of IV therapy (P < 0.001). The length of hospital stays and duration of IV antibiotics therapy was lower in the timely converted group than in the non-converted groups (P < 0.001). The duration of IV antibiotics therapy was strongly correlated with duration of hospital stay (r = 0.743, P < 0.001).
Conclusion:
The findings revealed a low prevalence of conversion from IV antibiotics to oral, despite a higher percentage of patients meeting the eligibility criteria for conversion. There is a need for the implementation of structured program to review patients on IV antibiotics and promote timely conversion to oral once they meet the conversion criteria.
The Asian corn borer, Ostrinia furnacalis (Guenée), emerges as a significant threat to maize cultivation, inflicting substantial damage upon the crops. Particularly, its larval stage represents a critical point characterised by significant economic consequences on maize yield. To manage the infestation of this pest effectively, timely and precise identification of its larval stages is required. Currently, the absence of techniques capable of addressing this urgent need poses a formidable challenge to agricultural practitioners. To mitigate this issue, the current study aims to establish models conducive to the identification of larval stages. Furthermore, this study aims to devise predictive models for estimating larval weights, thereby enhancing the precision and efficacy of pest management strategies. For this, 9 classification and 11 regression models were established using four feature datasets based on the following features geometry, colour, and texture. Effectiveness of the models was determined by comparing metrics such as accuracy, precision, recall, F1-score, coefficient of determination, root mean squared error, mean absolute error, and mean absolute percentage error. Furthermore, Shapley Additive exPlanations analysis was employed to analyse the importance of features. Our results revealed that for instar identification, the DecisionTreeClassifier model exhibited the best performance with an accuracy of 84%. For larval weight, the SupportVectorRegressor model performed best with R2 of 0.9742. Overall, these findings present a novel and accurate approach to identify instar and predict the weight of O. furnacalis larvae, offering valuable insights for the implementation of management strategies against this key pest.
At Saint George Hospital University Medical Center in Beirut, Lebanon, we determine (1) annual blood culture (BC) contamination (BCC) and utilization (BCU) rates vs international benchmarks, (2) identify blood culture contaminants, (3) bloodstream infections episodes in patients with and without COVID-19 after the pandemic onset, and (4) any epidemiologic trends in BCC and BCU.
Design:
Retrospective observational study.
Setting:
Private tertiary referral center, from January 1, 2010, to December 31, 2022.
Methods:
We define a contaminated BC as the growth of a typical contaminant/skin flora in 1-2/4 BC bottles. We calculate BCC rates as a percentage of the contaminated BC/total BC during the period and BCU rates as the number of BC/1000 patient days (PD).
Results:
The average BCU rate of 85.9/1000 PD in 2010–2019 increased to 106.6/1000 PD in 2020–2022. On average, patients with COVID-19 had a higher BCU rate of 185.9/1000 PD, corresponding to an additional 100 blood cultures/1000 PD. The average BCC rate was 7%, ranging from 6% in 2010–2019 to 8% in 2020–2022. We observed the highest BCC rate of 9% in patients with COVID-19, likely due to the higher BCU. The most frequently isolated contaminants were coagulase-negative Staphylococcus (96%), of which 65% were Staphylococcus epidermidis.
Conclusion:
We saw a multifactorial, persistently elevated rate of BCC over 13 years as unaffected by strict infection control practices. We think that further research targeting a standardized, low BCU rather than inevitable BCC while advocating for diagnostic stewardship of low-middle-income countries is essential, especially where the lack of appropriate resource allocation and awareness are problematic.
The earliest recorded observations of Antarctic icebergs occurred in 1688 and 1700 in the Gregorian New Style (NS) Calendar. The first sighting took place after Christmas 1687 in the Julian Old Style (OS) Calendar, when just north of the Antarctic Peninsula, Edward Davis observed “ice islands” with lengths of 5–10 km; the second occurred in February 1699 OS, when north of South Georgia, Edmond Halley observed and first sketched tabular icebergs. Although these were the earliest documented observations, because icebergs occur adjacent to New Zealand and South America, seagoing Māori and indigenous South Americans may have observed them eight centuries earlier. Davis and Halley’s observations were in the iceberg stream that flows to the east of the Antarctic Peninsula. Davis’s observations were the result of the Batchelor’s Delight being blown south from Cape Horn by a storm; his misadjusted compass meant they sailed east across instead of north through the stream. Comparison of Davis’s positions with satellite iceberg trajectories suggests his observations occurred at 62.5°S between 53.0° and 54.3°W. Davis assumed his icebergs were floating, but because Halley’s ice islands appeared stationary, he thought they were grounded, missing an opportunity to speculate on the existence of a southern ice-covered continent.
Parental surrogacy remains a highly controversial issue in contemporary ethics with considerable variation in the legal approaches of different jurisdictions. Finding a societal consensus on the issue remains highly elusive. John Rawls’ theory of public reason, first developed in his A Theory of Justice (1971), offers a unifying model of political discourse and engagement that enables reasonable citizens to accept policies that they do not necessarily support at a personal level. The theory established a promising framework for private citizens with distinct moral positions on the subject to find common ground and, in doing so, to negotiate a consensus regarding the degree and nature of regulation that is palatable to all rational citizens.
We present the case of a 31-year-old female with Fontan circulation who developed signs of protein-losing enteropathy 10 days after second COVID-19 vaccination. After standard investigations for identification of potential triggers for protein-losing enteropathy, we concluded that coronavirus disease 2019 (COVID-19) booster vaccination could have been the most probable underlying trigger. Prompt investigation of new symptoms post-vaccination in high-risk patients is necessary.
Plant secondary metabolites (PSMs) may improve gastrointestinal health by exerting immunomodulatory, anti-inflammatory and/or antiparasitic effects. Bark extracts from coniferous tree species have previously been shown to reduce the burden of a range of parasite species in the gastrointestinal tract, with condensed tannins as the potential active compounds. In the present study, the impact of an acetone extract of pine bark (Pinus sylvestris) on the resistance, performance and tolerance of genetically diverse mice (Mus musculus) was assessed. Mice able to clear an infection quickly (fast responders, BALB/c) or slowly (slow responders, C57BL/6) were infected orally with 200 infective third-stage larvae (L3) of the parasitic nematode Heligmosomoides bakeri or remained uninfected (dosed with water only). Each infection group of mice was gavaged for 3 consecutive days from day 19 post-infection with either bark extract or dimethyl sulphoxide (5%) as vehicle control. Oral administration of pine bark extract did not have an impact on any of the measured parasitological parameter. It did, however, have a positive impact on the performance of infected, slow-responder mice, through an increase in body weight (BW) and carcase weight and reduced feed intake by BW ratio. Importantly, bark extract administration had a negative impact on the fast responders, by reducing their ability to mediate the impact of parasitism through reducing their performance and tolerance. The results indicate that the impact of PSMs on parasitized hosts is affected by host's genetic susceptibility, with susceptible hosts benefiting more from bark extract administration compared to resistant ones.