For positive integers k and n, the shuffle group $G_{k,kn}$ is generated by the $k!$ permutations of a deck of $kn$ cards performed by cutting the deck into k piles with n cards in each pile, and then perfectly interleaving these cards following a certain permutation of the k piles. For $k=2$, the shuffle group $G_{2,2n}$ was determined by Diaconis, Graham and Kantor in 1983. The Shuffle Group Conjecture states that, for general k, the shuffle group $G_{k,kn}$ contains $\mathrm {A}_{kn}$ whenever $k\notin \{2,4\}$ and n is not a power of k. In particular, the conjecture in the case $k=3$ was posed by Medvedoff and Morrison in 1987. The only values of k for which the Shuffle Group Conjecture has been confirmed so far are powers of $2$, due to recent work of Amarra, Morgan and Praeger based on Classification of Finite Simple Groups. In this paper, we confirm the Shuffle Group Conjecture for all cases using results on $2$-transitive groups and elements of large fixed point ratio in primitive groups.