The objective of this experiment was to evaluate the resistance of advanced tomato genotypes resulting from an interspecific cross between Solanum lycopersicum and Solanum pennellii to Tetranychus urticae and Phthorimaea absoluta. The plant materials included nine lines, 14 hybrids, Leblon F1 (commercial control), and the wild accession S. pennellii LA716 as a resistance standard. Acylsugar content was then determined. For mite bioassays, oviposition, adult mortality/survival, egg hatching, and nymphs were evaluated using a no-choice bioassay. For P. absoluta bioassays, the oviposition, intensity of damage, type of lesions, and percentage of damaged leaflets were evaluated. F1 (TOM-808 × BPX-443E-03-02-113-02), F1(TOM-810 × BPX-443E-03-02-113-02), F1(TOM-808 × TOM-717), F1(TOM-808 × TOM-757), and F1(TOM-810 × TOM-717) were the most resistant to the mite, exhibiting higher female mortality, reduced oviposition, and no nymph emergence observed. The genotypes F1(TOM-808 × TOM-667), F1(TOM-808 × TOM-717), F1(TOM-810 × TOM-615), and several lines, which exhibited reduced oviposition and foliar damage. The results of the bioassays indicated that high acylsugar content reduced oviposition and foliar damage of the tested pests. The hybrid F1(TOM-808 × TOM-717) is the most promising at this stage of the breeding program, as it shows resistance to both tested pests.