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Exploring the efficacy and optimal dosages of omega-3 supplementation for companion animals

Published online by Cambridge University Press:  11 June 2025

Thiago Henrique Annibale Vendramini*
Affiliation:
Pet Nutrology Research Center, Department of Animal Nutrition and Production of the School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil Veterinary Nutrology Service, Teaching Veterinary Hospital of the School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil
Pedro Henrique Marchi
Affiliation:
Pet Nutrology Research Center, Department of Animal Nutrition and Production of the School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil
Rodrigo Fernando Gomes Olivindo
Affiliation:
Veterinary Nutrology Service, Teaching Veterinary Hospital of the School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil
Vivian Pedrinelli
Affiliation:
Veterinary Nutrology Service, Teaching Veterinary Hospital of the School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil
Andressa Rodrigues Amaral
Affiliation:
Veterinary Nutrology Service, Teaching Veterinary Hospital of the School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil
Mariana Santos de Miranda
Affiliation:
Pet Nutrology Research Center, Department of Animal Nutrition and Production of the School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil
Leonardo Andrade Príncipe
Affiliation:
Pet Nutrology Research Center, Department of Animal Nutrition and Production of the School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil
Cinthia Gonçalves Lenz Cesar
Affiliation:
Pet Nutrology Research Center, Department of Animal Nutrition and Production of the School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil
Rafael Vessecchi Amorim Zafalon
Affiliation:
Pet Nutrology Research Center, Department of Animal Nutrition and Production of the School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil
Mariana Pamplona Perini
Affiliation:
Pet Nutrology Research Center, Department of Animal Nutrition and Production of the School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil
Laís Oyama Cotrim Lima
Affiliation:
Veterinary Nutrology Service, Teaching Veterinary Hospital of the School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil
Júlio Cesar de Carvalho Balieiro
Affiliation:
Pet Nutrology Research Center, Department of Animal Nutrition and Production of the School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil
Marcio Antonio Brunetto
Affiliation:
Deceased
*
Corresponding author: Thiago Henrique Annibale Vendramini; Email: thiago.vendramini@usp.br

Abstract

This review summarises findings from studies in companion animals with chronic diseases receiving omega-3 supplementation. Investigated conditions included dermatopathies (dogs n = 7), osteoarthritis (dogs n = 7, cats n = 2), cardiovascular diseases (dogs n = 7), dyslipidaemias (dogs n = 1), gastroenteropathies (dogs n = 2), chronic kidney disease (dogs n = 2, cats n = 3), cognitive impairment (dogs n = 4, cats n = 1), and behavioural disorders (dogs n = 3). When possible, dosages were standardised to mg/kg using available data on food intake and EPA/DHA concentrations. The minimum and maximum ranges of EPA and DHA, along with their ratios, were as follows: for dermatology 0·99–43 mg/kg EPA and 0·66–30 mg/kg DHA (ratio 1·4–3·4); for osteoarthritis 48–100 mg/kg EPA and 20–32 mg/kg DHA (ratio 1·5–3·4); cardiology 27–54·2 mg/kg EPA and 18–40·6 mg/kg DHA (ratio 1·3–1·5); dyslipidaemia 58·8 mg/kg EPA and 45·4 mg/kg DHA (ratio 1·3); cognition (1/5 studies) 225 mg/kg EPA and 90 mg/kg DHA (ratio 2·5); behaviour (1/3) 31 mg/kg EPA and 45 mg/kg DHA (ratio 0·7). Nephrology and oncology studies lacked sufficient data for calculation. Gastrointestinal diseases do not appear to benefit from omega-3 supplementation, likely due to inflammation-related malabsorption, although few adverse effects were reported in dogs. Other enteropathy studies were low-quality (case reports/series). The lowest omega-6/omega-3 ratio with anti-inflammatory effect was 1:3·75, and the highest was 5·5:1. In conclusion, the reviewed EPA and DHA doses appear effective for atopic dermatitis, osteoarthritis, cardiac disease, hyperlipidaemia, and cognitive and behavioural disorders. Further research is needed to clarify efficacy in gastrointestinal and oncological conditions.

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Type
Review Article
Copyright
© The Author(s), 2025. Published by Cambridge University Press on behalf of The Nutrition Society

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References

Burr, GO, Burr, MM. (1930) On the nature and role of the fatty acids essential in nutrition. J. Biol. Chem 86, 587621.Google Scholar
Burr, GO, Burr, MM. (1929) A new deficiency disease produced by the rigid exclusion of fat from the diet. J. Biol. Chem 82, 345367.Google Scholar
Wesson, LG, Burr, GO. (1931) The metabolic rate and respiratory quotients of rats on a fat-deficient diet. J. Biol. Chem 91, 525539.Google Scholar
Hume, EM, Nunn, LCA, Smedley-Maclean, I, et al. (1938) Studies of the essential unsaturated fatty acids in their relation to the fat-deficiency disease of rats. Biochem. J 32, 21622177.Google Scholar
Spector, AA, Kim, H-Y. (2019) Emergence of omega-3 fatty acids in biomedical research. Prostaglandins, Leukot. Essent. Fat. Acids 140, 4750.Google Scholar
Anderson, RE, Maude, MB. (1970) Lipids of ocular tissues. 6. Phospholipids of bovine rod outer segments. Biochemistry 9, 36243628.Google Scholar
Benolken, RM, Anderson, RE, Wheeler, TG. (1973) Membrane fatty acids associated with the electrical response in visual excitation. Science (80-.) 182, 12531254.Google Scholar
Dyerberg, J, Bang, HO, Stoffersen, E, et al. (1978) Eicosapentaenoic acid and prevention of thrombosis and atherosclerosis? Lancet 312, 117119.Google Scholar
Lenox, C. (2016) Role of dietary fatty acids in dogs & cats. Today’s Vet Pract 8388.Google Scholar
Lourenço, AL, Booij-Vrieling, HE, Vossebeld, CB, et al. (2018) The effect of dietary corn oil and fish oil supplementation in dogs with naturally occurring gingivitis. J. Anim. Physiol. Anim. Nutr. (Berl) 102, 13821389.Google Scholar
Silva, DA, Nai, GA, Giuffrida, R, et al. (2018) Oral omega 3 in different proportions of EPA, DHA, and antioxidants as adjuvant in treatment of keratoconjunctivitis sicca in dogs. Arq. Bras. Oftalmol 81, 421428.Google Scholar
Trevizan, L, De Mello Kessler, A. (2009) Lipídeos na nutrição de cães e gatos: Metabolismo, fontes e uso em dietas práticas e terapêuticas. Rev. Bras. Zootec 38, 1525.Google Scholar
National Research Council. (2006) Nutrient requirements of dogs and cats. 1st ed. National Academy Press, editor. Nutr. Requir. Dogs Cats. Washington, D.C., D.C.: National Academies Press.Google Scholar
NIH. (2021) Omega-3 fatty acids fact sheet for consumers. Natl. Institutes Heal, 13.Google Scholar
Shahidi, F, Ambigaipalan, P. (2018) Omega-3 polyunsaturated fatty acids and their health benefits. Annu. Rev. Food Sci. Technol 9, 345381.Google Scholar
Bauer, JJE. (2008) Essential fatty acid metabolism in dogs and cats. Rev. Bras. Zootec 37, 2027.Google Scholar
Bauer, JE. (2011) Therapeutic use of fish oils in companion animals. J. Am. Vet. Med. Assoc 239, 14411451.Google Scholar
Dahms, I, Bailey-Hall, E, Sylvester, E, et al. (2019) Safety of a novel feed ingredient, Algal Oil containing EPA and DHA, in a gestation-lactation-growth feeding study in Beagle dogs. PLoS One 14(6), e0217794.Google Scholar
Bauer, JE. (2016) The essential nature of dietary omega-3 fatty acids in dogs. J. Am. Vet. Med. Assoc 249, 12671272.Google Scholar
Bauer, JE. (2006) Metabolic basis for the essential nature of fatty acids and the unique dietary fatty acid requirements of cats. J. Am. Vet. Med. Assoc 229, 17291732.Google Scholar
Hall, JA, Jackson, MI, Vondran, JC, et al. (2018) Comparison of circulating metabolite concentrations in dogs and cats when allowed to freely choose macronutrient intake. Biol Open Google Scholar
Stoeckel, K, Bachmann, L, Dobeleit, G, et al. (2013) Response of plasma fatty acid profiles to changes in dietary n-3 fatty acids and its correlation with erythrocyte fatty acid profiles in dogs. J. Anim. Physiol. Anim Nutr (Berl) 97, 11421151.Google Scholar
Adler, N, Schoeniger, A, Fuhrmann, H. (2018) Polyunsaturated fatty acids influence inflammatory markers in a cellular model for canine osteoarthritis. J. Anim. Physiol. Anim. Nutr. (Berl) 102, e62332.Google Scholar
Weylandt, KH, Chiu, C-Y, Gomolka, B, et al. (2012) Omega-3 fatty acids and their lipid mediators: towards an understanding of resolvin and protectin formation. Prostaglandins Other Lipid Mediat 97, 7382.Google Scholar
Zainal, Z, Longman, AJ, Hurst, S, et al. (2009) Relative efficacies of omega-3 polyunsaturated fatty acids in reducing expression of key proteins in a model system for studying osteoarthritis. Osteoarthr. Cartil 17, 896905.Google Scholar
Combarros, D, Castilla-Castaño, E, Lecru, LA, et al. (2020) A prospective, randomized, double blind, placebo-controlled evaluation of the effects of an n-3 essential fatty acids supplement (Agepi® ω3) on clinical signs, and fatty acid concentrations in the erythrocyte membrane, hair shafts and skin surface of dogs. Prostaglandins Leukot Essent Fat Acids 159.Google Scholar
Woldemeskel, M. (2019) Nutraceuticals in dermatological disorders. Nutraceuticals Vet. Med. 563568.Google Scholar
Mogensen, KM. (2017) Essential fatty acid deficiency. Pract. Gastroenterol 41, 3744.Google Scholar
Omega Fatty Acids, Stice SA.. (2019) Nutraceuticals Vet. Med. Cham: Springer International Publishing. p. 175185.Google Scholar
Logas, D, Kunkle, GA. (1994) Double-blinded crossover study with marine oil supplementation containing high-dose icosapentaenoic acid for the treatment of canine pruritic skin disease. Vet. Dermatol 5, 99104.Google Scholar
Halliwell, R. (2006) Revised nomenclature for veterinary allergy. Vet. Immunol. Immunopathol 114, 207208.Google Scholar
de Santiago, MS, Arribas, JLG, Llamas, YM, et al. (2021) Randomized, double-blind, placebo-controlled clinical trial measuring the effect of a dietetic food on dermatologic scoring and pruritus in dogs with atopic dermatitis. BMC Vet. Res 17, 354.Google Scholar
Olivry, T, Saridomichelakis, M, Nuttall, T, et al. (2014) Validation of the Canine Atopic Dermatitis Extent and Severity Index (CADESI)-4, a simplified severity scale for assessing skin lesions of atopic dermatitis in dogs. Vet Dermatol 25(2).Google Scholar
Olivry, T, DeBoer, DJ, Favrot, C, et al. (2010) Treatment of canine atopic dermatitis: 2010 clinical practice guidelines from the International Task Force on Canine Atopic Dermatitis. Vet. Dermatol 21, 233248.Google Scholar
Sævik, BK, Bergvall, K, Holm, BR, et al. (2004) A randomized, controlled study to evaluate the steroid sparing effect of essential fatty acid supplementation in the treatment of canine atopic dermatitis. Vet. Dermatol 15, 137145.Google Scholar
Paterson, S. (1995) Additive benefits of EFAs in dogs with atopic dermatitis after partial response to antihistamine therapy. J. Small Anim. Pract 36, 389394.Google Scholar
Müller, MR, Linek, M, Löwenstein, C, et al. (2016) Evaluation of cyclosporine-sparing effects of polyunsaturated fatty acids in the treatment of canine atopic dermatitis. Vet. J 210, 7781.Google Scholar
Abba, C, Mussa, PP, Vercelli, A, et al. (2005) Essential fatty acids supplementation in different-stage atopic dogs fed on a controlled diet. J. Anim. Physiol. Anim. Nutr. (Berl) 89, 203207.Google Scholar
Lenox, CE, Bauer, JE. Potential adverse effects of omega-3 fatty acids in dogs and cats. J. Vet. Intern. Med 27, 217226.Google Scholar
Magalhães, TR, Lourenço, AL, Gregório, H, et al. (2021) Therapeutic effect of EPA/DHA supplementation in neoplastic and non-neoplastic companion animal diseases: a systematic review. In Vivo (Brooklyn) 35, 14191436.Google Scholar
Barrouin-Melo, SM, Anturaniemi, J, Sankari, S, et al. (2016) Evaluating oxidative stress, serological- and haematological status of dogs suffering from osteoarthritis, after supplementing their diet with fish or corn oil. Lipids Health Dis 15(1).Google Scholar
Fritsch, DA, Allen, TA, Dodd, CE, et al. (2010) A multicenter study of the effect of dietary supplementation with fish oil omega-3 fatty acids on carprofen dosage in dogs with osteoarthritis. J. Am. Vet. Med. Assoc 236, 535539.Google Scholar
Roush, JK, Cross, AR, Renberg, WC, et al. (2010) Evaluation of the effects of dietary supplementation with fish oil omega-3 fatty acids on weight bearing in dogs with osteoarthritis. J. Am. Vet. Med. Assoc 236, 6773.Google Scholar
Roush, JK, Dodd, CE, Fritsch, DA, et al. (2010) Multicenter veterinary practice assessment of the effects of omega-3 fatty acids on osteoarthritis in dogs. J. Am. Vet. Med. Assoc 236, 5966.Google Scholar
Moreau, M, Troncy, E, del Castillo, JRE, et al. (2012) Effects of feeding a high omega-3 fatty acids diet in dogs with naturally occurring osteoarthritis. J Anim Physiol Anim Nutr (Berl).Google Scholar
Corbee, RJ, Barnier, MMC, van de Lest, CHA, et al. (2012) The effect of dietary long-chain omega-3 fatty acid supplementation on owner’s perception of behaviour and locomotion in cats with naturally occurring osteoarthritis. J Anim Physiol Anim Nutr (Berl).Google Scholar
Mehler, SJ, May, LR, King, C, et al. (2016) A prospective, randomized, double blind, placebo-controlled evaluation of the effects of eicosapentaenoic acid and docosahexaenoic acid on the clinical signs and erythrocyte membrane polyunsaturated fatty acid concentrations in dogs with osteoarthritis. Prostaglandins, Leukot. Essent. Fat. Acids 109, 17.Google Scholar
Abshirini, M, Ilesanmi-Oyelere, BL, Kruger, MC. (2021) Potential modulatory mechanisms of action by long-chain polyunsaturated fatty acids on bone cell and chondrocyte metabolism. Prog Lipid Res 83.Google Scholar
Patterson, E, Wall, R, Fitzgerald, GF, et al. (2012) Health implications of high dietary omega-6 polyunsaturated fatty acids. J. Nutr. Metab 2012.Google Scholar
Wu, CL, Jain, D, McNeill, JN, et al. (2015) Dietary fatty acid content regulates wound repair and the pathogenesis of osteoarthritis following joint injury. Ann. Rheum. Dis 74, 20762083.Google Scholar
Loef, M, Schoones, JW, Kloppenburg, M, et al. (2019) Fatty acids and osteoarthritis: different types, different effects. Jt. Bone Spine 86, 451458.Google Scholar
Hurst, S, Rees, SG, Randerson, PF, et al. (2009) Contrasting effects of n-3 and n-6 fatty acids on cyclooxygenase-2 in model systems for arthritis. Lipids 44, 889896.Google Scholar
Abramson, SB. (2008) Osteoarthritis and nitric oxide. Osteoarthr Cartil 16(SUPPL. 2).Google Scholar
Notoya, K, Jovanovic, D V., Reboul, P, et al. (2000) The induction of cell death in human osteoarthritis chondrocytes by nitric oxide is related to the production of prostaglandin E2 via the induction of cyclooxygenase-2. J. Immunol 165, 34023410.Google Scholar
Davies, PSE, Graham, SM, MacFarlane, RJ, et al. (2013) Disease-modifying osteoarthritis drugs: in vitro and in vivo data on the development of DMOADs under investigation. Expert Opin. Investig. Drugs 22, 423441. https://dx.doi.org/10.1517/13543784.2013.770837.Google Scholar
Sakata, S, Hayashi, S, Fujishiro, T, et al. (2015) Oxidative stress-induced apoptosis and matrix loss of chondrocytes is inhibited by eicosapentaenoic acid. J. Orthop. Res 33, 359365.Google Scholar
Buddhachat, K, Siengdee, P, Chomdej, S, et al. (2017) Effects of different omega-3 sources, fish oil, krill oil, and green-lipped mussel against cytokine-mediated canine cartilage degradation. Vitr. Cell. Dev. Biol. - Anim 53, 448457.Google Scholar
Zhang, T, Dai, Y, Zhang, L, et al. (2020) Effects of edible oils with different n-6/n-3 PUFA ratios on articular cartilage degeneration via regulating the NF-κB signaling pathway. J. Agric. Food Chem 68, 1264112650.Google Scholar
Shirai, Y, Morita, S, Iwata, T, et al. (2022) Anti-inflammatory and nutritional improvement effects of dietary supplementation combined with fish oil in patients with epithelial cancer. Oncol Lett 24(3).Google Scholar
Arends, J, Baracos, V, Bertz, H, et al. (2017) ESPEN expert group recommendations for action against cancer-related malnutrition. Clin. Nutr 11871196.Google Scholar
Ravasco, P. (2019) Nutrition in cancer patients. J Clin Med 8(8).Google Scholar
Weeth, LP, Fascetti, AJ, Kass, PH, et al. (2007) Prevalence of obese dogs in a population of dogs with cancer. J. Am. Vet. Med. Assoc 230, 11731173.Google Scholar
Michel, KE, Sorenmo, K, Shofer, FS. (2004) Evaluation of body condition and weight loss in dogs presented to a veterinary oncology service. J Vet Intern Med 18(5), 692695. https://doi.org/10.1892/0891-6640(2004)18<692:eobcaw>2.0.co;2. PMID: 15515586.2.0.co;2.+PMID:+15515586.>Google Scholar
Baez, JL, Michel, KE, Sorenmo, K, et al. (2007) A prospective investigation of the prevalence and prognostic significance of weight loss and changes in body condition in feline cancer patients. J. Feline Med. Surg 9, 411417.Google Scholar
Penna, F, Minero, VG, Costamagna, D, et al. (2010) Anti-cytokine strategies for the treatment of cancer-related anorexia and cachexia. Expert Opin. Biol. Ther. 12411250.Google Scholar
Seruga, B, Zhang, H, Bernstein, LJ, et al. (2008) Cytokines and their relationship to the symptoms and outcome of cancer Nat. Rev. Cancer.887899. https://dx.doi.org/10.1038/nrc2507.Google Scholar
Fearon, KCH. (2011) Cancer cachexia and fat–muscle physiology. N. Engl. J. Med 365, 565567.Google Scholar
Merlo, A, Rezende, BCG, Franchini, ML, et al. (2007) Serum C-reactive protein concentrations in dogs with multicentric lymphoma undergoing chemotherapy. J. Am. Vet. Med. Assoc 230, 522526.Google Scholar
Ogilvie, GK, Fettman, MJ, Mallinckrodt, CH, et al. (2000) Effect of fish oil, arginine, and doxorubicin chemotherapy on remission and survival time for dogs with lymphoma: a double-blind, randomized placebo- controlled study. Cancer 88, 19161928.Google Scholar
Tecles, F, Caldín, M, Zanella, A, et al. (2009) Serum acute phase protein concentrations in female dogs with mammary tumors. J. Vet. Diagnostic Investig 21, 214219.Google Scholar
Wei, L, Wu, Z, Chen, YQ. (2022) Multi-targeted therapy of cancer by omega-3 fatty acids-an update. Cancer Lett 526, 193204.Google Scholar
Hawcroft, G, Loadman, PM, Belluzzi, A, et al. (2010) Effect of eicosapentaenoic acid on E-type prostaglandin synthesis and EP4 receptor signaling in human colorectal cancer cells. Neoplasia 12, 618627.Google Scholar
McDaniel, JC, Massey, K, Nicolaou, A. (2011) Fish oil supplementation alters levels of lipid mediators of inflammation in microenvironment of acute human wounds. Wound Repair Regen 19 189200.Google Scholar
Petric, D. (2022) Immunonutrition: gut microbiota, glutamine and omega 3 polyunsatturated fatty acids. Food Ther. Heal. Care 4, 13.Google Scholar
McMillan, SK, Boria, P, Moore, GE, et al. (2011) Antitumor effects of deracoxib treatment in 26 dogs with transitional cell carcinoma of the urinary bladder. J. Am. Vet. Med. Assoc 239, 10841089.Google Scholar
Mohammed, SI, Bennett, PF, Craig, BA, et al. (2002) Effects of the cyclooxygenase inhibitor, piroxicam, on tumor response, apoptosis, and angiogenesis in a canine model of human invasive urinary bladder cancer. Cancer Res 62, 356358.Google Scholar
Soni, S, Torvund, M, Mandal, CC. (2021) Omega-3 fatty acid treatment combined with chemotherapy to prevent toxicity, drug resistance, and metastasis in Cancer. Curr. Drug Targets 23, 574596.Google Scholar
Freeman, LM, Rush, JE, Kehayias, JJ, et al. (1998) Nutritional alterations and the effect of fish oil supplementation in dogs with heart failure. J. Vet. Intern. Med 12, 440448.Google Scholar
Fulan, H, Changxing, J, Yi Baina, W, et al. (2011) Retinol, vitamins A, C, and e and breast cancer risk: A meta-analysis and meta-regression. Cancer Causes Control 22, 13831396. https://dx.doi.org/10.1007/s10552-011-9811-y.Google Scholar
Endo, J, Arita, M. (2016) Cardioprotective mechanism of omega-3 polyunsaturated fatty acids. J. Cardiol 67, 2227.Google Scholar
Keene, BW, Atkins, CE, Bonagura, JD, et al. (2019) ACVIM consensus guidelines for the diagnosis and treatment of myxomatous mitral valve disease in dogs. J. Vet. Intern. Med 33, 11271140.Google Scholar
Madsen, MB, Olsen, LH, Häggström, J, et al. (2011) Identification of 2 loci associated with development of myxomatous mitral valve disease in Cavalier King Charles Spaniels. J Hered 102(Suppl.).Google Scholar
Nasciutti, PR, Moraes, AT, Santos, TK, et al. (2021) Protective effects of omega-3 fatty acids in dogs with myxomatous mitral valve disease stages B2 and C. PLoS One 16(7 July).Google Scholar
von Haehling, S, Lainscak, M, Springer, J, et al. (2009) Cardiac cachexia: a systematic overview. Pharmacol. Ther 121, 227252.Google Scholar
Pasławski, R, Kurosad, A, Zabek, A, et al. (2021) Effect of 6-month feeding with a diet enriched in EPA + DHA from fish meat on the blood metabolomic profile of dogs with myxomatous mitral valve disease. Animals 11(12).Google Scholar
Smith, CE, Freeman, LM, Rush, JE, et al. (2007) Omega-3 fatty acids in boxer dogs with arrhythmogenic right ventricular cardiomyopathy. J. Vet. Intern. Med 21, 265273.Google Scholar
Laurent, G, Moe, G, Hu, X, et al. (2008) Long chain n-3 polyunsaturated fatty acids reduce atrial vulnerability in a novel canine pacing model. Cardiovasc. Res 77, 8997.Google Scholar
Freeman, LM. (2010) Beneficial effects of omega-3 fatty acids in cardiovascular disease. J. Small Anim. Pract 51, 462470.Google Scholar
Calder, PC. (2006) n-3 polyunsaturated fatty acids, inflammation, and inflammatory diseases. Am J Clin Nutr 83(6).Google Scholar
Simopoulos, AP. (2002) Omega-3 fatty acids in inflammation and autoimmune diseases. J. Am. Coll. Nutr 21, 495505.Google Scholar
Li, H, Ruan, XZ, Powis, SH, et al. (2005) EPA and DHA reduce LPS-induced inflammation responses in HK-2 cells: evidence for a PPAR-γ-dependent mechanism. Kidney Int 67, 867874.Google Scholar
Oh, DY, Talukdar, S, Bae, EJ, et al. (2010) GPR120 is an omega-3 fatty acid receptor mediating potent anti-inflammatory and insulin-sensitizing effects. Cell 142, 687698.Google Scholar
Figueras, M, Olivan, M, Busquets, S, et al. (2011) Effects of eicosapentaenoic acid (EPA) treatment on insulin sensitivity in an animal model of diabetes: Improvement of the inflammatory status. Obesity 19, 362369.Google Scholar
Golub, N, Geba, D, Mousa, SA, et al. (2011) Greasing the wheels of managing overweight and obesity with omega-3 fatty acids. Med. Hypotheses 77, 11141120.Google Scholar
Lorente-Cebrián, S, Costa, AGV, Navas-Carretero, S, et al. Role of omega-3 fatty acids in obesity, metabolic syndrome, and cardiovascular diseases: a review of the evidence. J. Physiol. Biochem 69, 633651.Google Scholar
de Albuquerque, P, de Marco, V, Vendramini, THA, et al. (2021) Supplementation of omega-3 and dietary factors can influence the cholesterolemia and triglyceridemia in hyperlipidemic Schnauzer dogs: a preliminary report. PLoS One 16(October).Google Scholar
Xenoulis, PG, Steiner, JM. (2010) Lipid metabolism and hyperlipidemia in dogs. Vet. J. P.G 183, 1221.Google Scholar
ADA. (2011) Diagnosis and classification of diabetes mellitus. Diabetes Care 34(SUPPL.1).Google Scholar
Catchpole, B, Adams, JP, Holder, AL, et al. (2013) Genetics of canine diabetes mellitus: Are the diabetes susceptibility genes identified in humans involved in breed susceptibility to diabetes mellitus in dogs? Vet. J 195, 139147.Google Scholar
Behrend, E, Holford, A, Lathan, P, et al. (2018) 2018 AAHA diabetes management guidelines for dogs and cats. J. Am. Anim. Hosp. Assoc 54, 121.Google Scholar
Shoelson, S, Lee, J, Goldfine, A. (2006) Inflammation and insulin resistance. J Clin Invest 116, 17931801.Google Scholar
Salles, BCC, Terra, MC, Paula, FB de A. (2019) Sinalização mediada pela insulina em vias anabólicas. Gen. Pharm. J 1, 2545.Google Scholar
Tsalamandris, S, Antonopoulos, AS, Oikonomou, E, et al. (2019) The role of inflammation in diabetes: current concepts and future perspectives. Eur. Cardiol. Rev 14, 5059.Google Scholar
Calder, P. (2018) XI International Conference on Immunonutrition 2018: ISIN. Ann. Nutr. Metab. 73, 184226.Google Scholar
Calder, PC. (2017) Omega-3 fatty acids and inflammatory processes: from molecules to man. Biochem. Soc. Trans 45, 11051115.Google Scholar
Brunetto, MA, Sa, FC, Nogueira, SP, et al. (2011) The intravenous glucose tolerance and postprandial glucose tests may present different responses in the evaluation of obese dogs. Br. J. Nutr 106, S1947.Google Scholar
Mead, JR, Irvine, SA, Ramji, DP. (2002) Lipoprotein lipase: structure, function, regulation, and role in disease. J. Mol. Med 80, 753769.Google Scholar
Arnaldi, G, Scandali, VM, Trementino, L, et al. (2010) Pathophysiology of dyslipidemia in Cushing’s syndrome. Neuroendocrinology 92, 8690.Google Scholar
Kersten, S. (2008) Peroxisome proliferator activated receptors and lipoprotein metabolism. PPAR Res. Google Scholar
Lamaziere, A, Wolf, C, Barbe, U, et al. (2013) Lipidomics of hepatic lipogenesis inhibition by omega 3 fatty acids. Prostaglandins Leukot. Essent. Fat. Acids 88, 149154.Google Scholar
Mach, F, Baigent, C, Catapano, AL, Koskinas, KC, Casula, M, Badimon, L, et al. (2019ESC/EAS guidelines for the management of dyslipidaemias: lipid modification to reduce cardiovascular risk. Eur Heart J 41(1), 111188.Google Scholar
Kenar, L, Karayilanoglu, T, Aydin, A, et al. (2008) Protective effects of diets supplemented with omega-3 polyunsaturated fatty acids and calcium against colorectal tumor formation. Dig. Dis. Sci. 53, 21772182.Google Scholar
Wei, J-J, Tang, D-P, Xie, J-J, et al. (2016) Decreased n-6/n-3 polyunsaturated fatty acid ratio reduces chronic reflux esophagitis in rats. Prostaglandins Leukot. Essent. Fat. Acids 112, 3743.Google Scholar
Gobbetti, T, Dalli, J, Colas, RA, et al. (2017) Protectin D1n-3 DPA and resolvin D5n-3 DPA are effectors of intestinal protection. Proc. Natl. Acad. Sci. U. S. A. 114, 39633968.Google Scholar
Ungaro, F, Rubbino, F, Danese, S, et al. (2017) Actors and factors in the resolution of intestinal inflammation: Lipid mediators as a new approach to therapy in inflammatory bowel diseases. Front. Immunol 8.Google Scholar
Ajabnoor, SM, Thorpe, G, Abdelhamid, A, et al. (2021) Long-term effects of increasing omega-3, omega-6 and total polyunsaturated fats on inflammatory bowel disease and markers of inflammation: a systematic review and meta-analysis of randomized controlled trials. Eur. J. Nutr 60, 22932316.Google Scholar
Heilmann, RM, Suchodolski, JS. (2015) Is inflammatory bowel disease in dogs and cats associated with a Th1 or Th2 polarization? Vet. Immunol. Immunopathol 168, 131134.Google Scholar
Cerquetella, M, Spaterna, A, Laus, F, et al. (2010) Inflammatory bowel disease in the dog: differences and similarities with humans. World J. Gastroenterol 16, 10501056.Google Scholar
Davenport, DJ, Jergens, AE, Remmilard, R. (2010) Inflammatory bowel disease. In: Hand MS, Thatcher CD, Remillard RL, et al., editors. Small Anim. Clin. Nutr. 5th ed. Topeka: Mark Morris Institute. p. 1065–76.Google Scholar
Washabau, RJ, Day, MJ, Willard, MDD, et al. (2010) Endoscopic, biopsy, and histopathologic guidelines for the evaluation of gastrointestinal inflammation in companion animals. J. Vet. Intern. Med. 24, 1026.Google Scholar
Lei, QC, Wang, XY, Xia, XF, et al. (2015) The role of omega-3 fatty acids in acute pancreatitis: a meta-analysis of randomized controlled trials. Nutrients 7, 22612273.Google Scholar
Kim, JK, Lee, KS, Lee, DK, et al. (2014) Omega-3 polyunsaturated fatty acid and ursodeoxycholic acid have an additive effect in attenuating diet-induced nonalcoholic steatohepatitis in mice. Exp Mol Med 46(12).Google Scholar
Kilchoer, B, Vils, A, Minder, B, et al. (2020) Efficacy of dietary supplements to reduce liver fat. Nutrients 12, 116.Google Scholar
Loftus, JP, Miller, AJ, Center, SA, et al. (2021) Treatment and outcomes of dogs with hepatocutaneous syndrome or hepatocutaneous-associated hepatopathy. J. Vet. Intern. Med. 110.Google Scholar
Bouchier, IA. (1992) The formation of gallstones. Keio J. Med 41, 15.Google Scholar
Jang, SI, Fang, S, Kim, KP, et al. (2019) Combination treatment with n-3 polyunsaturated fatty acids and ursodeoxycholic acid dissolves cholesterol gallstones in mice. Sci Rep 9(1).Google Scholar
Walter, R, Dunn, ME, D’Anjou, M-A, et al. (2008) Nonsurgical resolution of gallbladder mucocele in two dogs. J. Am. Vet. Med. Assoc. 232, 16881693.Google Scholar
Sharma, M, Sharma, R, McCarthy, ET, et al. (2017) Hyperfiltration-associated biomechanical forces in glomerular injury and response: potential role for eicosanoids. Prostaglandins Other Lipid Mediat 132, 5968.Google Scholar
Daenen, K, Andries, A, Mekahli, D, et al. (2019) Oxidative stress in chronic kidney disease. Pediatr. Nephrol. 34, 975991.Google Scholar
Gyurászová, M, Gurecká, R, Bábíčková, J, et al. (2020) Oxidative stress in the Pathophysiology of kidney disease: implications for noninvasive monitoring and identification of biomarkers. Oxid. Med. Cell. Longev 111.Google Scholar
Brown, SA, Brown, CA, Crowell, WA, et al. (2000) Effects of dietary polyunsaturated fatty acid supplementation in early renal insufficiency in dogs. J. Lab. Clin. Med 135, 275286.Google Scholar
Brown, SA, Brown, CA, Crowell, WA, et al. (1998) Beneficial effects of chronic administration of dietary ω-3 polyunsaturated fatty acids in dogs with renal insufficiency. J. Lab. Clin. Med 131, 447455.Google Scholar
Brown, SA. (2008) Oxidative stress and chronic kidney disease. Vet. Clin. North Am. Small Anim. Pract 38, 157166.Google Scholar
Hall, JA, Yerramilli, M, Obare, E, et al. (2014) Comparison of serum concentrations of symmetric dimethylarginine and creatinine as kidney function biomarkers in healthy geriatric cats fed reduced protein foods enriched with fish oil, L-carnitine, and medium-chain triglycerides. Vet. J 202, 588596.Google Scholar
Hall, JA, Jackson, MI, Farace, G, et al. (2019) Influence of dietary ingredients on lean body Percent, Uremic Toxin Concentrations, and Kidney function in senior-adult cats. Metabolites 9, 238.Google Scholar
Hall, JA, Brockman, JA, Davidson, SJ, et al. (2017) Increased dietary long-chain polyunsaturated fatty acids alter serum fatty acid concentrations and lower risk of urine stone formation in cats. PLoS One. 12, e0187133.Google Scholar
Youdim, KA, Martin, A, Joseph, JA. (2000) Essential fatty acids and the brain: possible health implications. Int. J. Dev. Neurosci 18, 383399.Google Scholar
Anderson, GJ, Neuringer, M, Lin, DS, et al. (2005) Can prenatal N-3 fatty acid deficiency be completely reversed after birth? Effects on retinal and brain biochemistry and visual function in rhesus monkeys. Pediatr. Res 58, 865872.Google Scholar
Innis, SM. (2008) Dietary omega 3 fatty acids and the developing brain. Brain Res 1237, 3543.Google Scholar
Barceló-Coblijn, G, Murphy, EJ. (2009) Alpha-linolenic acid and its conversion to longer chain n-3 fatty acids: benefits for human health and a role in maintaining tissue n-3 fatty acid levels. Prog. Lipid Res 48, 355374.Google Scholar
Dinicolantonio, JJ, O’keefe, JH. (2020) The importance of marine OMEGA-3S for brain development and the prevention and treatment of behavior, mood, and other brain disorders. Nutrients 12, 115.Google Scholar
Serhan, CN, Chiang, N, Van Dyke, TE. (2008) Resolving inflammation: Dual anti-inflammatory and pro-resolution lipid mediators. Nat. Rev. Immunol 8, 349361.Google Scholar
Valenzuela, A, Nieto, S, Sanhueza, J, et al. (2010) Supplementing female rats with DHA-lysophosphatidylcholine increases docosahexaenoic acid and acetylcholine contents in the brain and improves the memory and learning capabilities of the pups. Grasas y Aceites 61, 1623.Google Scholar
DeGiorgio, CM, Taha, AY. (2016) Omega-3 fatty acids (ῳ-3 fatty acids) in epilepsy: animal models and human clinical trials. Expert Rev. Neurother 16, 11411145.Google Scholar
Dumas, C, Doré, FY. (1989) Cognitive development in kittens (Felis catus): a cross-sectional study of object permanence. J. Comp. Psychol 103, 191200.Google Scholar
Beynen, A. (2017) Brain food for puppies. Creat. companion 10, 3638.Google Scholar
Hemmings, C. (2019) Nutrition for kittens. Vet. Nurse 10, 250256.Google Scholar
AAFCO. (2019) Association of American Feed Control Officials. Washington, DC: Official Publication;.Google Scholar
FEDIAF. (2022) Nutritional Guidelines for Complete and Complementary Pet Food for Cats and Dogs. Nutr. Guidel. - Complet. Complement. Pet Food a Cats Dogs. Belgium: Bruxelles: The European Pet Food Industry Federation;(August):96.Google Scholar
Kaur, H, Singla, A, Singh, S, et al. (2020) Role of omega-3 fatty acids in canine health: a review. Int. J. Curr. Microbiol. Appl. Sci 9, 22832293.Google Scholar
Pawlosky, RJ, Denkins, Y, Ward, G, et al. (1997) Retinal and brain accretion of long-chain polyunsaturated fatty acids in developing felines: the effects of corn oil-based maternal diets. Am. J. Clin. Nutr 65, 465472.Google Scholar
Pan, Y, Araujo, JA, Burrows, J, et al. (2013) Cognitive enhancement in middle-aged and old cats with dietary supplementation with a nutrient blend containing fish oil, B vitamins, antioxidants and arginine. Br. J. Nutr 110, 4049.Google Scholar
Dang, R, Zhou, X, Tang, M, et al. (2018) Fish oil supplementation attenuates neuroinflammation and alleviates depressive-like behavior in rats submitted to repeated lipopolysaccharide. Eur. J. Nutr 57, 893906.Google Scholar
Vuorinen, A, Bailey-Hall, E, Karagiannis, A, et al. (2020) Safety of algal oil containing EPA and DHA in cats during gestation, lactation and growth. J. Anim. Physiol. Anim. Nutr. (Berl) 104, 15091523.Google Scholar
Kelley, RL, Lepine, AJ. (2014) Un supplemento di acido docoesanoico (DHA) nella dieta post-svezzamento potenzia le capacità d’apprendimento dei cuccioli in crescita. Aivpa J.Google Scholar
Heinemann, KM, Waldron, MK, Bigley, KE, et al. (2005) Long-chain (n-3) polyunsaturated fatty acids are more efficient than α-linolenic acid in improving electroretinogram responses of puppies exposed during gestation, lactation, and weaning. J. Nutr 135, 19601966.Google Scholar
Zicker, SC, Jewell, DE, Yamka, RM, et al. (2012) Evaluation of cognitive learning, memory, psychomotor, immunologic, and retinal functions in healthy puppies fed foods fortified with docosahexaenoic acid-rich fish oil from 8 to 52 weeks of age. J. Am. Vet. Med. Assoc 241, 583594.Google Scholar
Pan, Y, Kennedy, AD, Jönsson, TJ, et al. (2018) Cognitive enhancement in old dogs from dietary supplementation with a nutrient blend containing arginine, antioxidants, B vitamins and fish oil. Br. J. Nutr 119, 349358.Google Scholar
Della Giustina, A, Goldim, MP, Danielski, LG, et al. (2020) Fish oil–rich lipid emulsion modulates neuroinflammation and prevents long-term cognitive dysfunction after sepsis. Nutrition 70.Google Scholar
Hadley, KB, Bauer, J, Milgram, NW. (2017) The oil-rich alga Schizochytrium sp. as a dietary source of docosahexaenoic acid improves shape discrimination learning associated with visual processing in a canine model of senescence. Prostaglandins Leukot. Essent. Fat. Acids 118, 1018.Google Scholar
Taha, AY, Henderson, ST, Burnham, WM. (2009) Dietary enrichment with medium chain triglycerides (ac-1203) elevates polyunsaturated fatty acids in the parietal cortex of aged dogs: Implications for treating age-related cognitive decline. Neurochem. Res 34, 16191625.Google Scholar
Pan, Y, Landsberg, G, Mougeot, I, et al. (2018) Efficacy of a therapeutic diet on dogs with signs of cognitive dysfunction syndrome (CDS): a prospective double blinded placebo controlled clinical study. Front. Nutr 5, 127.Google Scholar
Araujo, JA, Segarra, S, Mendes, J, et al. (2022) Sphingolipids and DHA improve cognitive deficits in aged beagle dogs. Front Vet Sci 9.Google Scholar
Ferrari, D, Cysneiros, RM, Scorza, CA, et al. (2008) Neuroprotective activity of omega-3 fatty acids against epilepsy-induced hippocampal damage: quantification with immunohistochemical for calcium–binding proteins. Epilepsy Behav 13, 3642.Google Scholar
Scorza, FA, Cavalheiro, EA, Arida, RM, et al. (2009) Positive impact of omega-3 fatty acid supplementation in a dog with drug-resistant epilepsy: a case study. Epilepsy Behav 15, 527528.Google Scholar
Matthews, H, Granger, N, Wood, J, et al. (2012) Effects of essential fatty acid supplementation in dogs with idiopathic epilepsy: a clinical trial. Vet. J 191, 396398.Google Scholar
Luchtman, DW, Meng, Q, Song, C. (2012) Ethyl-eicosapentaenoate (E-EPA) attenuates motor impairments and inflammation in the MPTP-probenecid mouse model of Parkinson’s disease. Behav. Brain Res 226, 386396.Google Scholar
Chang, CY, Kuan, YH, Li, JR, et al. (2013) Docosahexaenoic acid reduces cellular inflammatory response following permanent focal cerebral ischemia in rats. J. Nutr. Biochem 24, 21272137.Google Scholar
Casali, BT, Corona, AW, Mariani, MM, et al. (2015) Omega-3 fatty acids augment the actions of nuclear receptor agonists in a mouse model of alzheimer’s disease. J. Neurosci 35, 91739181. https://dx.doi.org/10.1523/JNEUROSCI.1000-15.2015.Google Scholar
Grosso, G, Micek, A, Marventano, S, et al. (2016) Dietary n-3 PUFA, fish consumption and depression: a systematic review and meta-analysis of observational studies. J. Affect. Disord 205, 269281.Google Scholar
Bourre, JM. (2004) Roles of unsaturated fatty acids (especially omega-3 fatty acids) in the brain at various ages and during ageing. J. Nutr. Heal. Aging 8, 163174.Google Scholar
Ephraim, E, Brockman, JA, Jewell, DE. (2022) A diet supplemented with polyphenols, prebiotics and omega-3 fatty acids modulates the intestinal microbiota and improves the profile of metabolites linked with anxiety in dogs. Biology (Basel) 11, 976.Google Scholar
Sechi, S, Chiavolelli, F, Spissu, N, et al. (2015) An antioxidant dietary supplement improves brain-derived Neurotrophic factor levels in serum of aged dogs: preliminary results. J. Vet. Med 2015, 19.Google Scholar
Rahimi Niyyat, M, Azizzadeh, M, Khoshnegah, J. (2018) Effect of supplementation with omega-3 fatty acids, magnesium, and zinc on canine behavioral disorders: results of a pilot study. Top. Companion Anim. Med 33, 150155.Google Scholar
van den Berg, L. (2005) Structure and variation of three canine genes involved in serotonin binding and transport: the serotonin receptor 1A gene (htr1A), serotonin receptor 2A gene (htr2A), and serotonin transporter gene (slc6A4). J. Hered 96, 786796.Google Scholar
Re, S, Zanoletti, M, Emanuele, E. (2008) Aggressive dogs are characterized by low omega-3 polyunsaturated fatty acid status. Vet. Res. Commun 32, 225230.Google Scholar
Rees, A-M, Austin, M-P, Parker, G. (2005) Role of Omega-3 fatty acids as a treatment for depression in the Perinatal Period. Aust. New Zeal. J. Psychiatry 39, 274280.Google Scholar
Saker, KE, Eddy, AL, Thatcher, CD, et al. (1998) Manipulation of dietary (n-6) and (n-3) fatty acids alters platelet function in cats. J Nutr 128, 2645S2647S.Google Scholar
Bright, JM, Sullivan, PS, Melton, SL, et al. (1994) The effects of n-3 fatty acid supplementation on bleeding time, plasma fatty acid composition, and in vitro platelet aggregation in cats. J Vet Intern Med 8, 247252.Google Scholar
Boudreaux, MK, Reinhart, GA, Vaughn, DM, et al. (1997) The effects of varying dietary n-6 to n-3 fatty acid ratios on platelet reactivity, coagulation screening assays, and antithrombin III activity in dogs. J Am Anim Hosp Assoc 33, 235243.Google Scholar
LeBlanc, CJ, Bauer, JE, Hosgood, G, et al. (2005) Effect of dietary fish oil and vitamin E supplementation on hematologic and serum biochemical analytes and oxidative status in young dogs. Vet Ther 6, 325340.Google Scholar
McNiel, EA, Ogilvie, GK, Mallinckrodt, C, et al. (1999) Platelet function in dogs treated for lymphoma and hemangiosarcoma and supplemented with dietary n-3 fatty acids. J Vet Intern Med 13, 574580.Google Scholar
Bond, R, Lloyd, DH. (1992) Randomized single-blind comparison of an evening primrose oil and fish oil combination and concentrates of these oils in the management of canine atopy. Vet. Dermatol 3, 215219.Google Scholar
Bond, R, Lloyd, DH. (1993) Double-blind comparison of three concentrated essential fatty acid supplements in the management of canine atopy. Vet. Dermatol 4, 185189.Google Scholar
Lascelles, BDX, DePuy, V, Thomson, A, et al. (2010) Evaluation of a therapeutic diet for Feline degenerative joint disease. J. Vet. Intern. Med 24, 487495.Google Scholar
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