Knowledge regarding gene bank accessions characters facilitates the identification of the most promising for future use. Sampling of accessions from a cultigen available at a gene bank may be based on diversity and variability analysis relying on characterization or evaluation data, DNA markers, or both. Core subsets may aid selection by guiding users to genetic differences. Evaluation results must be shared promptly and widely worldwide, so germplasm users can request them for further utilization in plant breeding or research on plant genetic resources. This chapter relates therefore to gathering accurate and precise evaluation of diverse accessions in well-designed trials. Such knowledge is essential for identifying the most relevant accessions for further use. This information needs to be shared widely and quickly with users to achieve a maximum impact because any germplasm user should be aware of the potential of gene bank accessions in order to fully exploit such plant genetic resources.This chapter also illustrates the need for an objective assessment of gene bank accessions, deals with sampling of core subsets for evaluation of gene bank accessions, explains the principles of experimental design for trials of gene bank accessions and provides basic knowledge regarding trial data analysis.

Conservation planning involves selecting target taxa, establishing the geographic and taxonomic breadth of the conservation actions and producing some form of strategy or action plan for implementation. The selection of which taxon or taxa to conserve should be based on a series of measurable criteria such as the threat of genetic erosion / extinction, value of the taxon to humankind or the ecosystem, or the potential ease of use of the taxon or taxa. Once the target taxon is chosen, its geographic distribution, habitat preferences, phenology, and taxonomy are established to identify gaps in existing conservation actions to formulate an effective conservation strategy. Gap analysis is used to analyse the literature, passport data on gene bank accessions, label information on herbarium specimens for each taxon, as well as consulting taxonomic experts, and databases and concludes with strategic action to enhance conservation. Increasingly these strategic actions are tested in terms of their mitigation of climate change for the target taxon / taxa and formulated into a national strategy and action plan that gives background to their selection and clearly establishes the required conservation actions needed. The success of these actions is then measured against biodiversity indicators, so progress can be reviewed.

Data management is key to conservation and this includes: the types of data, how data are recorded, data standardization and conservation data management. The main sources of data are passport (provenance) and ecogeographic data, characterization and evaluation data, and management or curatorial data, each of these data are collated and managed by conservationists. Although associated with these data management types, three fundamentally important pieces of data are pivotal. The accession number (or unique identifier), the scientific name of the taxon being conserved and the date of the conservation intervention (e.g. seed collection or population monitoring date). Often today data management is assisted by using a conservation data management system (e.g. GRIN Global) to improve efficiency and help enforce data standardization. Further data management is assisted by use of data collection templates or accession / population descriptors. Much of these data are then made available to the user community via web-based platforms such as EURISCO or GBIF. The importance of access to and ownership of the various kinds of data is emphasized as fundamental to good data management practice.

Conserving plant genetic diversity for further use by crossbreeding through various methods for crop improvement is an important goal. Indeed, plant germplasm remains the most important genetic resource for sustaining crossbreeding. Advances in cell and molecular biology enable us to further exploit inter-genera and interfamily variation along with capitalizing on intra-specific and inter-specific variation. Hence, this chapter describes the theory underpinning population genetics and how to use this knowledge for providing a sound and effective strategy and ensuing plan of work for collecting, conserving and using plant genetic diversity. The reader of this chapter will get enough knowledge to identify a suitable crossbreeding method to develop a new cultivar or to improve a breeding line or population and assess the feasibility of using recent advances in agro-biotechnology for transferring new traits into available cultivars or breed new cultivars. This acquired knowledge will allow understanding the basic principles of plant breeding and thereafter use them for crossing and selecting segregating offspring with desired characters.

An understanding of taxonomy is fundamental to effective conservation planning and implementation. Associated knowledge is used in the selection of species for conservation, understanding inter-taxon relationships and field identification. At its heart is the elucidation of relationships between taxa (families, genera, species or subspecific taxa) and the production of classifications that reflect their evolutionary relationships. The relationships are summarized in the classification, based commonly on analysis of molecular and / or morphological characters, and the included taxa are placed in a taxonomic hierarchy with decreasing relatedness or similarity between the ranks as you go up the hierarchy. Once the classification is established a series of other products can be derived for the included taxa such as descriptions, synonyms, distribution maps, identification aids and accepted nomenclature.

On-farm conservation involves the maintenance of traditional varieties by farmers in agroecosystems. The important role of small farmers living and cultivating in complex, diverse and risk-prone marginal and heterogeneous environments in maintaining crop diversity on-farm is described. The on-farm conservation of plant genetic resources is complex because there are many factors that will influence a farmer’s decision on the management of their crops and fields, which in turn affect the quantity and quality of the inherent genetic diversity. The multiple private and public values and benefits of on-farm conservation are highlighted, as well as the options and interventions that can help strengthen the role of small farmers and farming communities in on-farm conservation. A major focus for the conservationist or development practitioner will be to encourage the farmer to continue cultivation of traditional varieties and this may be achieved by niche marketing, seed shows, participatory traditional variety improvement or even financial incentives. Key steps that need to be considered when implementing an on-farm conservation project are outlined and a brief review of the impact of on-farm conservation projects and the implications for scaling up actions is provided.

This chapter provides an introduction and overview of how to plan and undertake the collection of plant genetic samples in the field, and once collected how the samples should be processed, stored and made available for utilization. As such it includes a practical overview of expedition planning and of how to collect germplasm, voucher specimen and passport data. Germplasm collection involves the sampling and ex situ conservation of seed, tubers, cuttings, corms and vegetative plants away from the site where the material was originally located. The types of collection sites, and how each may be sampled are reviewed. The special cases of collecting fruit or other trees, forages, wild species, vegetative material and indigenous knowledge are discussed. A model for a generalized germplasm collection form and a collection report structure is presented, and the processing and final deposition of the conserved germplasm is summarized.

There is a strong social and ethicalpolicy context to plant genetic resources conservation and use. This is achieved by providing the historical background, introducing the major conventions, treaties and agreements, as well as the key stakeholders from the formal and informal sectors that are concerned with plant conservation, use and the equitable sharing of benefits. An attempt is made to assess the impact of the CBD and other conventions and to provide a balanced summary of the continuing debate on key issues such as ex situ versus in situ conservation, hotspots of botanical diversity and ex situ conservation and plant breeders’ rights, farmers’ rights, access, release of transgenic plants and sustainability both in conservation and use.