The fungus Beauveria bassiana (Unioeste 76) was tested against the soybean pest Euschistus heros in laboratory, greenhouse, and field. In the laboratory, insects were sprayed with pure conidia (TC) suspended in distilled water or in an oil dispersion formulation (OD; vegetable oil) at a concentration of 109 conidia/mL. The UV-B radiation and heat tolerance of the conidia were also assessed. After 12 days, the mortality rates in the laboratory were 70% for the TC treatment and 80% for the OD treatment. In the greenhouse pre-infestation bioassay, which used soybean plants in cages, the fungal treatments resulted in 52% and 47% mortality for the TC and OD formulations, respectively. In the post-infestation bioassay, both fungal treatments caused 83% mortality. In the field trial conducted on soybean plots (14 × 18 m), the treatments included: (i) biological: OD (109 conidia/mL); (ii) chemical insecticide; (iii) biological + chemical, all applied at 150 L/ha. Insect numbers were evaluated using beating-sheet sampling. In the final population sample, the biological treatment showed a population density similar to the chemical treatment (0.94 and 0.83 insects/m, respectively), both below the economic threshold. Conidia tolerance to UV-B radiation was similar across both treatments, but conidia in oil were less tolerant to heat. These results suggest that strategically combining both approaches (B. bassiana with chemical insecticides), with careful consideration of application intervals, could provide a sustainable and effective method for managing natural populations of E. heros.

While increasing seafood consumption may help address micronutrient deficiencies and metabolic disorders, evidence supporting this recommendation in the Indian context remains limited and inconclusive. Using the nationally representative cross-sectional 2019–2021 National Family Health Survey dataset, we investigated the association of fish consumption frequency with anaemia and metabolic disorders (overweight/obesity, hypertension and hyperglycaemia) among adult men (aged 15–54 years) and women (aged 15–49 years) in India. A control function (CF) method was employed to examine the association in individuals who consumed fish daily and those who reported consuming fish daily/weekly. The analysis was restricted to non-vegetarians (who reported ever consuming egg, fish or meat). Overall, 86·9 % of men and 74·7 % of women were non-vegetarians. CF analysis revealed that both daily and daily/weekly fish consumption were associated with a reduced risk of anaemia among both men and women. Daily fish consumers exhibited increased likelihood of overweight/obesity (men: β: 0·405, 95 % CI: 0·074, 0·735, P: 0·017; women: β: 0·248, 95 % CI 0·125, 0·370, P < 0·001). Conversely, daily/weekly fish intake was associated with a reduced risk of overweight/obesity in men (β: −0·041, 95 % CI: −0·069, −0·013; P: 0·004). Daily/weekly fish consumption was associated with a reduced risk of hypertension and increased odds of hyperglycaemia among men. Fish consumption demonstrated a potentially protective relationship against hypertension in women, regardless of how often they consumed fish, while also being associated with a higher prevalence of hyperglycaemia. Indian adults can improve their health by eating more fish, which can help fight anaemia and may also reduce overweight/obesity and high blood pressure.

India, as the world’s most populous country, and with a substantial urban population, requires strategic development to mitigate the risks of urban pluvial flooding in the context of a changing climate. Rapid urbanization increases the presence of impervious surfaces, and climate change effects bring intense, frequent and long-duration rainfall events in India, which magnify urban flooding. Implementing sustainable urban drainage solutions (SUDSs) would mitigate stormwater flood risks, but India has yet to adopt this approach; instead, it relies on traditional drainage infrastructure, despite increasing population indices and an extended yearly rainfall season. Here, we highlight the existing scenario, the challenges and the way forward towards implementing SUDSs in India. To attain SUDSs, city-specific drainage-related challenges need to be identified through problem tree analysis, co-creation with stakeholders of a shared vision for sustainable urban drainage and the design of actionable pathways and experimental approaches for implementing interventions and refining practical indicators. These actions could collectively provide a roadmap for achieving resilient SUDSs.

Butachlor is a herbicide extensively employed in rice (Oryza sativa L.) cultivation but historically under-investigated for its toxicological impacts on terrestrial vegetation. This study examines the dose-dependent effects of butachlor on the germination and antioxidant defense mechanisms in the seeds of Asian tape grass [Vallisneria natans (Lour.) H. Hara], an important submerged plant species widely distributed in the agricultural ponds. In a hydroponic setup, seeds were exposed to four concentrations of butachlor (0, 20, 200, and 2,000 μg ai L−1), and cultivated under controlled light conditions to quantify germination rates and assess oxidative stress responses. Our findings showed that butachlor concentrations up to 20 μg L−1 had no effect on the germination rate of V. natans seeds, while germination rates decreased by 6.0% and 8.7% at 200 and 2,000 μg L−1, respectively. At 2,000 μg L−1, malondialdehyde (MDA) content increased by 5.7 nmol g−1 FW, and catalase (CAT) activity declined by 21%, indicating oxidative damage. Additionally, the antioxidants proline (Pro) and glutathione (GSH) were upregulated under 20 μg L−1 butachlor treatment after 12 h, contributing to reactive oxygen species (ROS) scavenging and cellular stability. This study highlights the nuanced interactions between butachlor exposure and the antioxidant defenses in V. natans, providing valuable insights into the ecological impacts of herbicide pollution. Understanding these interactions is crucial for development of sustainable agricultural practices and management of herbicide resistance in aquatic systems.

Each year, over 100,000 dogs are imported into Germany from other EU countries by animal welfare organisations, mainly from Romania. This study conducted a systematic content analysis of websites belonging to 241 animal welfare organisations that rehome dogs from southern and eastern Europe to Germany. Assessment included transparency, legal compliance, and availability of educational and medical information for adopters. The study shows that many organisations lacked information regarding dogs’ origins, transport methods, or health status which sometimes makes it difficult to distinguish between dog rescue and illegal dog trade. Photos were mainly used in adoption advertisements and behavioural descriptions were only included in two-thirds of the dog advertisements which poses a risk of increased dog relinquishment post-adoption due to behavioural unsuitability. Information on vector-borne diseases and typical behaviour of imported rescue dogs was not provided comprehensively. Few organisations violated legal standards, offering underage or banned breeds and failing to use the TRACES transport system. Most organisations relied upon private foster homes, while few had no temporary housing available in Germany. Although most claimed to conduct pre-adoption checks, comprehensive contract details were rarely published. The number of existing animal welfare organisations that rehome dogs from southern and eastern Europe to Germany is unable to be determined due to high fluctuation and the lack of central registry. Inadequate health disclosures and behavioural descriptions risk poor adoption matches and increased returns. Lack of legal compliance may endanger both animal and public welfare and opens the door to illegal dog trade. Sustainable animal protection requires better adopter education, reliable medical testing, and local engagement in source countries to reduce reliance upon transnational rehoming.

One of the most incredible aspects of the Mexican Baja Peninsula is the immense wealth of plant and animal diversity it holds. The human communities living alongside this richness have boundless intimate knowledge of its natural history, potentially with novel insights into the ecological and evolutionary processes shaping the diversity of plants and animals. These same human communities have likely also witnessed changes to these natural environments over their lifetimes, particularly as the effects of global change are being felt by similar rural communities around the world. However, because the area is so remote, they often have little access to scientific data or current information about the causes or effects of the changes they observe. Using a thematic analysis of recorded conversations, this project seeks to connect remote rural ranches in Mexico with scientists, to gather data on the issues that matter most to the community members, and work to find collaborative solutions. Through thematic analysis of recorded conversations, our research reveals that unpredictable climate variability, including droughts, hurricanes, and shifting seasonal patterns, poses significant challenges to ranching livelihoods. Ranchers’ deep ecological knowledge provides critical insights into the stresses of changing and increasingly unpredictable environmental trends. By integrating local perspectives with scientific approaches, this study highlights the potential for collaborative biodiversity research.

Twin children are more likely to die than singletons. This is an additional burden in sub-Saharan African (SSA) countries, as child mortality levels are already higher than anywhere else. This article provides estimates of under-5 mortality rates (U5MRs) for twins and singletons in SSA from 1986 to 2016. It describes the geographical variations and changes over time. It also describes the variation of twins’ excess mortality according to age from 0 to 5 years. Additionally, it analyzes the factors associated with twins’ excess mortality. We used data from 156 national surveys from 42 countries. We estimated U5MRs for twins and single children and built a Cox model to analyze factors associated with excess mortality among twins. Although child mortality has declined on the continent, twins’ excess mortality remains very high. U5MRs are, on average, 3 times higher among twins than singletons. The Cox model shows that all other things being equal, the adjusted hazard ratio of under-5 mortality (U5M) is 3.2 (2.9−3.3; p < .001) times higher among twins than singletons. The main factors associated with excess mortality risks among twins are biomedical and nutritional features, such as low birth weight, non-use of cesarean section delivery, and lack of breastfeeding. Health policy makers in SSA should be aware of the vulnerability of twins, and interventions to prevent their early deaths should be considered.

With the introduction of tetflupyrolimet as the first herbicide with a novel site of action in the last three decades, screening for herbicide resistance before commercialization has become integral to ensure successful applications. In the mid-southern United States, tetflupyrolimet is anticipated to be used as a preemergence (PRE) herbicide for barnyardgrass control but does exhibit postemergence (POST) herbicidal activity. In 2020, 45 Echinochloa crus-galli (barnyardgrass) accessions were collected from rice-producing areas in Arkansas and were screened in the greenhouse to tetflupyrolimet at 134 g ai ha-1 PRE and POST at the 2- to 3-leaf growth stage on a silt loam soil. A field experiment was conducted where tetflupyrolimet was applied alone at 134 g ai ha-1 or with clomazone at 336 g ai ha-1, to a susceptible barnyardgrass standard and four other accessions with confirmed resistance to florpyrauxifen-benzyl, imazethapyr, propanil, and quinclorac at the spiking, 1-, 2-, 3-, and 4-leaf stages. For the PRE screening, the percent visible control ranged from 88% to 99%, with some accessions differing in sensitivity to tetflupyrolimet. Percent mortality ranged from 47% to 90% at the PRE timing. Visible control and mortality ranged from 63% to 88% and 7% to 65%, respectively, from a POST application, suggesting there is differential sensitivity and that foliar applications may not be as effective as soil applications. In the field experiment, barnyardgrass accession did not influence POST biomass production and was impacted more by the growth stage at application, although the difference was frequently numerical. In general, applying tetflupyrolimet alone or with clomazone to ≥3 leaf grass compromised performance. Tetflupyrolimet will be better optimized as a soil-applied herbicide in mid-southern U.S. rice culture.

Sitodiplosis mosellana (Géhin) (Diptera: Cecidomyiidae) is a significant pest of wheat, Triticum aestivum Linnaeus (Poaceae), in Canada. Monitoring currently relies on labour-intensive counts of ovipositing females. Although traps baited with S. mosellana pheromone are used as decision support tools in the United Kingdom, in Canada, they are considered reliable only to indicate adult activity. Recent findings show that variability in pheromone release from commercial lures affects the number of midges captured and limits the reliability of pheromone monitoring. Here, two lure types and two trap types were compared for their ability to attract and retain S. mosellana males. We then compared the number of males captured in pheromone traps with the information provided by other monitoring tools, including emergence traps, soil cores, and ovipositing female counts. Jackson traps with Trécé rubber septa lures captured the most midges. The number of males captured in pheromone-baited traps was not related to overwintering, ovipositing, or emerging populations, suggesting that pheromone traps may not accurately reflect S. mosellana populations under field conditions. Variability in extracted pheromone amount between lures, regional climate, and Canada’s vast wheat-growing area may limit the development of an effective pheromone-based decision support tool for this region. Nevertheless, refinement of lure formulation, standardisation of trapping protocols, and integration of complementary monitoring approaches may enhance trap reliability and support a stronger monitoring system.

Pelmatozoa is an informal grouping of filter-feeding echinoderms including crinoids, paracrinoids, rhombiferans, and eocrinoids that possess a theca, an erect stalk, and feeding appendages. Although crinoids were major constituents of marine communities with high diversity and abundance throughout the Paleozoic, most other pelmatozoans had relatively low species diversity and/or short temporal durations. It has been proposed that these different diversification trajectories could have resulted from crinoids outcompeting other filter-feeding pelmatozoans during the early Paleozoic, although this hypothesis involving niche overlap has never been formally tested. Here, we tested this hypothesis using the incredibly diverse pelmatozoan fauna of the Late Ordovician (Sandbian) Bromide Formation of Oklahoma, which preserves a rich, ecologically complex fauna that developed as a result of the Great Ordovician Biodiversification Event. We developed a framework to quantitatively characterize pelmatozoan feeding ecology using multivariate analysis of ecomorphological traits and explored niche space occupation and potential competition between crinoids, rhombiferans, paracrinoids, eocrinoids, and diploporans from the Bromide fauna. Results revealed key ecological factors controlling niche differentiation and showed that crinoids, paracrinoids, and rhombiferans occupy nonoverlapping regions of niche space, indicating competition between groups was unlikely. Although the competition hypothesis was not supported, narrow niche space occupation suggests that paracrinoids and rhombiferans were more ecologically limited than crinoids, which might have played a role in their differential diversification dynamics. These results elucidate both the nature of interactions between pelmatozoan taxa and the potential mechanisms driving their evolutionary trajectories, as well as the complexity of ecological communities that arose during the Ordovician radiation.

Although studies had suggested that monosodium glutamate (MSG) may serve as a salt substitute in processed foods, its market impact remains unclear. This study was aimed to: (1) identify MSG in ingredient lists, (2) compare sodium levels in products with and without MSG, (3) examine the association between MSG and other umami enhancers, and (4) assess manufacturers’ adherence to the Food Regulation 1985 for MSG declaration. An observational cross-sectional market survey was conducted during the Movement Control Order (June to September 2021) on 1076 processed foods from selected Malaysian hypermarkets, that were located within 10 km of the researcher’s residence to ensure accessibility amid the COVID-19 pandemic movement restrictions. Additionally, online platforms were used to supplement any missing data. Nutritional and ingredient information were collected from packaging, and foods were classified by using the FAO/WHO Food Standards CODEX Alimentarius. Over half (50.8%) of the surveyed products contained MSG. Results found that MSG-containing products had a significantly higher sodium levels (2812.2 ± 5191.4 mg/100 g) than those without (1372.2 ± 1541.4 mg/100 g) (p < 0.001). Additionally, 14.4% of MSG-free products were labelled as ‘No Added MSG’, while products with MSG were more likely to contain other umami enhancers (p < 0.001). Compliance with MSG declaration regulations was 61%. In conclusion, MSG was present in half of the surveyed products. MSG-containing products were more likely to have higher sodium content and include other flavour enhancers. Future research should analyse the actual MSG as well as sodium content and explore alternative sodium reduction strategies.

Ensuring seed viability over decades is a central challenge in ex situ conservation of plant genetic resources. Standard germination tests, while effective, are destructive and fail to detect early molecular damage that precedes viability loss. RNA integrity has emerged as a promising biomarker due to the inherent vulnerability of mRNA to oxidative degradation in the dry state. In this study, we identified and validated seed-stored mRNAs in common bean (Phaseolus vulgaris), a major crop species extensively represented in global germplasm collections, and investigated transcript degradation patterns in seeds conserved for up to 30 years. Using a comparative genomics approach, we identified 107 P. vulgaris orthologs of long-lived rice mRNAs, many encoding proteins involved in RNA stabilization, oxidoreductase activity, and primary metabolism. Thirty transcripts were validated by RT-qPCR, and the integrity of nine was assessed using paired primers targeting 5′ and 3′ regions. Degradation followed a consistent 5′→3′ pattern, particularly in longer transcripts, and correlated strongly with germination power. Predictive performance varied across transcripts: PSMA4, SMP1, and TRA2 consistently showed strong correlations with viability, whereas others were less informative. The ΔΔCq metric improved resolution by detecting degradation asymmetry. Samples included a genetically diverse panel of conserved, regenerated, and artificially aged accessions, enhancing applicability to real-world genebank conditions. Artificial aging intensified degradation signatures and mirrored patterns observed in naturally aged seeds. Altogether, our results indicate transcript integrity as a molecular biomarker for seed viability, supporting the development of robust molecular tools to inform decision-making and regeneration planning in long-term germplasm conservation.

The global use of antimicrobial chemicals drastically increased during and after the COVID-19 pandemic owing to heightened awareness of personal and surface hygiene needs. Disinfectants, especially chlorine-based disinfectants (CBDs), were extensively used for surface and equipment decontamination in the domestic, industrial, veterinary and healthcare sectors during the heights of the pandemic. The increased use of disinfectants has resulted in their increased discharge into municipal wastewater systems and surface waters. Our Perspective article considers the One Health challenges associated with the increased discharge of disinfectants into wastewater. One Health is a collaborative approach that ensures the well-being of people, animals and the environment. Wastewater is a common endpoint to the many interactions between people, animals and their environment. The potential One Health challenges and knowledge gaps associated with the constant discharge of low but sublethal concentrations of CBDs into wastewater are discussed. The data gaps point to the risks associated with the unregulated use of CBDs and need for their judicial use.