Pummelo is the largest citrus species in the Rutaceae family and is important for human health and nutrition. This fruit is underutilized and threatened by human activities in its natural habitat. The present research was conducted in the Devanahalli region of Karnataka, which has a vast diversity of pummelo. This study aimed to evaluate 40 pummelo genotypes, selected through a preliminary survey, using morphological, biochemical and sensory assessments. Fruit and pulp weights ranged from 344.03 to 1746.23 g and 194.39 to 1073.07 g, with mean values of 939.67 and 522.81 g, respectively. Traits such as fruit length, diameter, fruit axis diameter, fruit volume, number of segments, rind thickness, fruit, pulp, peel weight, seed weight and seeds per fruit showed highly significant differences. Biochemical analysis revealed that ascorbic acid content varied from 28.53 to 60 mg/100 g, titratable acidity from 0.73 to 2.07% and total soluble solids from 7.63 to 11.07°Brix. High phenol (55.08–166.17 mg GAE/g FW) and flavonoid (39.26–137.78 mg CE/g FW) content were observed in fruit pulp, indicating significant antioxidant potential. Several morphological and biochemical traits exhibited significant positive correlations. A slightly higher phenotypic coefficient of variation was found for all the characters compared to the corresponding genotypic coefficient of variation. The principal component analysis indicated that the first nine components contributed 94.24% of the total variation. Hierarchical cluster analysis divided the populations into two distinct clusters. Sensory analysis indicated that genotypes, DVP-1 and DVP-2 had higher acceptability rates. The significant variations in plant traits among the genotypes offer great chances to select the best ones for genetic improvement programmes.