Luminescence dating and profiling are important analytical methods for providing chronological constraints and reconstructing depositional histories from sediment cores. However, sediment cores have often been exposed to ionising radiation sources during geophysical analyses, which potentially contaminates natural luminescence signals and may compromise the accuracy and reliability of luminescence analyses. Variable water content down-core is another potential issue for the rapid analysis of sediments, as water attenuates luminescence and may limit the comparability of samples. Here, we use a portable optically stimulated luminescence reader to test the influence of two common geophysical analyses—X-radiography and gamma-ray logging—on the luminescence properties of sediments in marine cores. We demonstrate that both techniques cause negligible changes to luminescence signals with doses <100 mGy. We test the effect of variable water content on luminescence and show that net signals are reduced by up to 70% at 30% moisture, relative to dry sediments. Accurate and reliable luminescence signals can be obtained from sediment cores despite prior exposure to ionising radiation from geophysical loggers or variable water content. However, the accuracy of luminescence measurements does require taking appropriate steps before analysis, like assessing the doses given by geophysical instruments at specific laboratories or drying samples.