Accurate radiation dose measurement is crucial for medical intervention and protective actions. Biological dose assessment directly measures radiation-induced molecular and physiological changes, providing information about the absorbed dose and potential health risks. The Korea Institute of Radiological and Medical Sciences (KIRAMS) has performed biological dosimetry using cytogenetic assays since 2010. These assays are used for individual dose estimation in various situations, including occupational exposure, accidental radiation exposure, and health risk assessment of people living near nuclear power plants in Korea. Recent advancements in biological dose assessment methods, such as automated scoring and high-throughput assays, have improved efficiency and enabled more people to undergo dose assessment. The KIRAMS continuously explores new methods and targets for biodosimetry to enhance dose assessment capabilities and can contribute to expand the biological dose assessment capacity with the expertise and facilities, responding to large-scale accidents of radiation exposure in the world.