Palmer amaranth is a troublesome weed in cotton production. Yield losses of65% have been reported from season-long Palmer amaranth competition withcotton. To determine whether water is a factor in this system, experimentswere conduced in 2011, 2012, and 2013 in Citra, FL, and in Tifton, GA. In2011, infrequent rainfall lead to drought stress. The presence of Palmeramaranth resulted in decreased soil relative water content up to 1 m indepth. Cotton stomatal conductance (gs) was reduced up to 1.8 m from a Palmer amaranth plant. In 2012and 2013 higher than average rainfall resulted in excess water throughoutthe growing season. In this situation, no differences were found in soilrelative water content or cotton gs as a function of proximity to Palmer amaranth. A positivelinear trend was found in cotton photosynthesis and yield; each parameterincreased as distance from Palmer amaranth increased. Even in thesewell-watered conditions, daily water use of Palmer amaranth was considerablyhigher than that of cotton, at 1.2 and 0.49 g H20 cm−2 d−1, respectively. Although Palmer amaranth removed more waterfrom the soil profile, rainfall was adequate to replenish the profile in 2of the 3 yr of this study. However, yield loss due to Palmer amaranth wasstill observed despite no change in gs, indicating other factors, such as competition for light orresponse to neighboring plants during development, are driving yieldloss.