While animal welfare is a growing global concern, there has been very little research into how it is understood in Iran. Cultural, religious, and legal factors influence attitudes and practices in ways not addressed by existing research. This study provides culturally grounded insights for improvement of animal welfare in Iran. Utilising a validated survey tool, we investigated the attitudes of Iranians toward the welfare of farmed, companion, and wild animals. A total of 325 responses were collected. The findings indicate that animal welfare is considered important to Iranians, with the majority expressing interest in improving the welfare practices. Despite varying degrees of familiarity with different animal species, there was a consensus on the importance of enacting laws to protect animal welfare. Most participants agreed that chickens feel pain (92.9%) and emotions (79%), whereas fewer attributed these capacities to fish, with 63.6% acknowledging pain and 59.5% acknowledging emotions. Furthermore, most of the participants agreed that animals should not endure pain in the slaughter process (97.8% agreement). While the majority of participants agreed that pre-slaughter stunning was better for the animals (78.7%), only 51.7% agreed that they would prefer to eat meat from animals that had been stunned; reflecting the traditionally held views regarding the role of stunning in Halal meat production. The results of the current study support previous findings suggesting that concern for animals may be a universal human inclination, although, in Iran, attitudes towards specific species and agricultural practices are also shaped by religious perspectives.