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Selection of the best embryo to transfer, that is, the one that will have the highest potential to result in a live birth, is one of the most important decisions in an in vitro fertilization (IVF) laboratory. A zygote forms with the amalgamation of the female and male gametes, and much information about the viability of an embryo can be gained within 16 to 20 hours after insemination. Interaction of the oocyte and sperm should first be examined with an accurate assessment of fertilization.
This chapter will discuss the techniques for fertilization verification for oocytes fertilized both conventionally and by intracytoplasmic sperm injection (ICSI). The formation of the pronuclei is the first indication of the fusion of male and female gametes. Practical considerations and laboratory protocols are detailed in the chapter.
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