Introduction
From the beginning of history, there have been repeated claims of the observation of unusual life forms (i.e., extraterrestrial life), which have not been confirmed by the scientific community owing to a lack of credible evidence. Since the second half of the 20th century, advanced technologies (such as space probes, radio telescopes, electronic processors, and biological/biochemical techniques) have become much more accurate and reliable in exploring for life in the most distant parts of the universe (Bada, Reference Bada2001).
In general, evidence for extraterrestrial life in recent years has focused mainly on the following: (1) Biochemical studies of meteorites and the search for traces of fossilized microorganisms or organic molecules in living structures (such as nucleic acid building blocks as genetic information repositories) (Rozanov et al., Reference Rozanov, Hoover, Krasavin, Samylina, Ryumin and Kapralov2021; Oba et al., Reference Oba, Takano, Furukawa, Koga, Glavin, Dworkin and Naraoka2022). (2) Images and information (including paleontology and atmospheric conditions) obtained from various planets and astronomical objects by space probes (such as Perseverance, Venus, Galileo, etc.) have enabled man to uncover the habitability of other celestial bodies in the solar system to a certain extent (Girija, Reference Girija2023). (3) Observations of radio telescopes that receive the waves emitted by cosmic objects and phenomena and display them on their screens. By analyzing the information received by radio telescopes, it is not only possible to identify various stellar objects but also to obtain valuable information about their nature and conditions, which can be used to roughly estimate the habitability of heavenly bodies (Song et al., Reference Song2023). (4) Claims and documents related to extraterrestrial intelligence and civilization that have been repeatedly reported in different periods of history (Rayhan, Reference Rayhan2023).
In recent years, artificial intelligence (AI) has become a crucial tool for analyzing complex scientific data (https://www.nasa.gov; Rodriguez et al., Reference Rodriguez, Tejani, Pydipalli and Patel2018). In astrobiology, the vast amount of data collected from space probes, meteorite spectroscopy, and radio observations presents significant analytical challenges (Cleaves et al., Reference Cleaves, Hystad, Prabhu, Wonga, Cody, Economon and Hazen2023; Durrani, Reference Durrani2024). Machine learning algorithms and deep neural networks can process these massive datasets and identify hidden patterns that traditional methods might overlook (Charbonneau, Reference Charbonneau2024). For instance, AI models can accurately detect biosignatures in meteorite spectroscopy or distinguish anomalous radio signals – potentially of intelligent origin – from cosmic background noise (Davies et al., Reference Davies, Benner, Cleland, Lineweaver, McKay and Wolfe-Simon2009; Durrani, Reference Durrani2024).
Furthermore, AI has the potential to push the search for extraterrestrial life beyond current frontiers. In the future, autonomous AI-powered probes could explore extreme environments (Hein, Reference Hein2016), such as the subsurface of Mars or the oceans of icy moons like Europa and Enceladus, analyzing biosignatures in real time without human intervention. Additionally, AI could play a pivotal role in analyzing suspicious radio and laser signals that might indicate extraterrestrial civilizations, enhancing the probability of detection (Durrani, Reference Durrani2024). With the advancement of AI technology, astrobiology stands on the brink of a new era of faster, more precise, and potentially groundbreaking discoveries that could revolutionize our understanding of life in the universe. In the following, we will discuss each of these claims on extraterrestrial life and explore the role of artificial intelligence in enhancing discoveries related to extraterrestrial life.
Signs of extraterrestrial life in meteorites
With the advancement of biological/chemical techniques in the second half of the 20th century, studies on meteorites were carried out with much greater accuracy and under more suitable laboratory conditions than before. The results of these studies led to controversial evidence for the presence of biogenic hydrocarbon compounds and microfossils in the Orgueil meteorite, biogenic nanofossils in the Martian meteorite ALH84001, and amino acids and nucleotides in the carbonaceous chondrites Murchison. At the time, astronomers and meteorologists, who had little knowledge of microbiology and microfossilology, strongly rejected the claims about this evidence. In the absence of sufficient instruments to prove such a claim, the rejection or confirmation of extraterrestrial microscopic life in meteorites remained silent for two decades (Rozanov et al., Reference Rozanov, Hoover, Krasavin, Samylina, Ryumin and Kapralov2021). With the advent of modern chemical/biological analysis techniques, the evidence for extraterrestrial life in meteorites gradually strengthened. The identification of DNA and RNA subunits in the components of the seven-billion-year-old Murchison meteorite (Oba et al., Reference Oba, Takano, Furukawa, Koga, Glavin, Dworkin and Naraoka2022) has strengthened the likelihood that the subunits making up life have an extraterrestrial origin.
Orgueil meteorite
In 1864, in the southwest of France, people witnessed a fiery ball in the sky, which created a long smoky streak on its way down. Suddenly, this ball exploded at a height of 30 km in the sky and its pieces hit the ground in an area of 20 × 4 km in the village of Orgueil (Figure 1). The strong noise and the white cloud created in the path of movement led to the tracking of the impact area of the meteorite. Immediately, many reports and documents were collected from the crash site and the samples were transferred to the meteorite collection of the National Museum of Natural History in Paris and Montauban, and the Imperial Observatory research institution at the PSL University (Rozanov et al., Reference Rozanov, Hoover, Krasavin, Samylina, Ryumin and Kapralov2021).

Figure 1. A large fragment of the Orgueil meteorite in the National Museum of Natural History in France.
A preliminary chemical analysis of the Orgueil meteorite revealed that it is a chondrite rich in carbon and organic matter and contains 8–10% extraterrestrial water in its matrix, together with ammonium and magnesium salts, anhydrite, calcite, serpentine, pyrrhotite, silica and a water-insoluble polymeric carbon complex and coal. In later tests, the chemical compounds of this meteorite were determined more precisely (Table 1) (Rozanov et al., Reference Rozanov, Hoover, Krasavin, Samylina, Ryumin and Kapralov2021).
Table 1. Chemical compounds of the Orgueil meteorite (Rozanov et al., Reference Rozanov, Hoover, Krasavin, Samylina, Ryumin and Kapralov2021)

About 14% of Orgueil’s chemical compounds consist of organic substances, some of which can be produced by biological or chemical processes. However, some of them are strong biomarkers such as amino acids, nucleobases, and some molecules derived from the decomposition of chlorophyl, such as porphyrin. The reason why they are considered strong biomarkers is that these types of molecules are not produced by any of the non-biological mechanisms and at the same time their structure can be millions old, even up to billions of years. Among the amino acids identified in this meteorite, the amino acids glycine, alanine, aspartic acid, glutamic acid, leucine and proline were observed.
From the observation of life-related chemical compounds in the Orgueil meteorite, two researchers named Nagy et al. (Reference Nagy, Meinschein and Hennessy1961) decided to study this meteorite more closely from the perspective of microbiology. The initial results of these two scientists were very interesting and indicated the discovery of all kinds of particles with microscopic sizes and similar to fossil algae, which they interpreted as possible fossils of microorganisms. These two researchers put forward the following arguments and presented some explanations: (1) The high number of possible microfossil particles in the Orgueil (1,650–1,700/mg) can only exist if liquid water was in constant contact with the meteorite. Of course, in the subsequent tests, it was found that the analyzed components of the meteorite disintegrate rapidly in water. (2) The organized elements that were very similar to the microfossils mostly resembled some terrestrial microscopic organisms (especially dinoflagellates and chrysomonads), which belong to the protozoa found in aquatic environments such as seas and lakes. (3) Possible microfossils in the Orgueil meteorite were similar to another meteorite called Ivuna, which fell at different times and in another climatic region. This challenges the possibility of terrestrial origin for the found microfossils (Figures 2, 3) (Rozanov et al., Reference Rozanov, Hoover, Krasavin, Samylina, Ryumin and Kapralov2021).

Figure 2. Microscopy of the organized elements in the Orgueil meteorite (Rozanov et al., Reference Rozanov, Hoover, Krasavin, Samylina, Ryumin and Kapralov2021).

Figure 3. Microfossil with a structure resembling a magnetotactic bacterium in the Orgueil meteorite (Rozanov et al., Reference Rozanov, Hoover, Krasavin, Samylina, Ryumin and Kapralov2021). Magnetotactics are a group of bacteria that can produce nanostructures called magnetosomes, and this leads to the alignment of these microorganisms in the magnetic field. In this image, structures such as the magnetosome can be seen in the dark along the pseudo-microfossil structure.
These interpretations led to a hot debate at the time about the possible origin of these pseudo-microfossil structures. Shortly after Nagy and Hennessey claimed to have found evidence of microfossils in the Orgueil meteorite, two other researchers named Anders and Fitch (Reference Anders and Fitch1962) contradicted this claim and attributed the pseudo-microfossil structures to some factors including mineral processes or contamination with plant pollens during fell to Earth the meteorite. Until the end of the 20th century, many other scientists also disagreed with the argument that “the similarity of morphology cannot be regarded as valid evidence for the assignment of organized elements to microfossils.” Many other researchers also rejected the possibility of contamination at the crash site or even in the museum (Rozanov et al., Reference Rozanov, Hoover, Krasavin, Samylina, Ryumin and Kapralov2021). Discussion on this topic continued into the second decade of the 21st century until Aerts et al. (Reference Aerts and Elsaesser2016) dismissed the possibility of contamination of the Orgueil meteorite with plant pollen particles or other terrestrial contaminants, which led to the observation of forms resembling microfossils or strong biomarkers. They proved that the strong biomarkers and the organized structures are not of terrestrial origin and were generated from the Orgueil meteorite when it detached from the parent body.
The probability of extraterrestrial life in the Orgueil meteorite was strengthened by the detection of strong biomarker molecules and pseudo-microfossil structures. These findings not only strengthen evidence for the existence of pseudo-terrestrial microbial life in Earth-like celestial bodies but also largely confirm the panspermia hypothesis. From this theory, in the early stages of the Earth’s formation, the seeds of life in the form of primary single cells were brought to the ground of this planet by meteorites. After the original population multiplied and spread, these single-celled organisms spread to different parts of the Earth depending on the climatic and environmental conditions. As a result of natural selection over billions of years, they have divided into various groups of organisms. However, there is still a long way to go before the panspermia hypothesis is finally proven. Not only should more studies be carried out on similar samples, but also the discovery of organic life forms on other celestial bodies can help to prove this hypothesis.
Murchison meteorite
At the end of September 1969, a large meteorite was observed in the sky above the town of Markinson in southeastern Australia, that was broken up into various pieces by a cloud of smoke, and its pieces fell to the ground over an area of 13 square kilometers (Fuchs et al., Reference Fuchs, Olsen and Jensen1973). After collection, the largest part of this meteorite (65 kg) was sent to the Field Museum of Natural History in Chicago. Other pieces were given to the Smithsonian Institution’s National Museum of Natural History in Washington, DC (30 kg), the Museum of Australia in Sydney (about 4 kg), and the College of Melbourne in Australia (7 kg). Smaller quantities, about a few kilograms, were also given to museums, universities, research institutes, and even private individuals (Figure 4).

Figure 4. A fragment of the Murchison meteorite.
This celestial stone with a lifespan of 7 billion years is the oldest material discovered by mankind. Murchison belongs to the carbonaceous chondrite meteorites and black silicate is the most important mineral (with 77% volume and 27.22% weight). The total water content is about 12% and the carbon content is 1.6–2.2% of its weight. Table 2 lists the relative amount of chemical compounds in the Murchison meteorite (Fuchs et al., Reference Fuchs, Olsen and Jensen1973).
Table 2. Microscopy of the elements in the Murchison (Fuchs et al., Reference Fuchs, Olsen and Jensen1973)

From the 1960s, shortly after the collection of Murchison’s pieces, the search for signs of life in some of his pieces began. In the first endeavor, organic compounds such as amino acids (subunits for building proteins) were proven in this meteorite (Cronin and Moore, Reference Cronin and Moore1971). However, the extraterrestrial origin of these molecules could not be confirmed. Due to the feasibility that the meteorite was contaminated with terrestrial organic molecules after the fall, it was difficult to prove the nature of the amino acids. This confusion continued until Koga and Naraoka (Reference Koga and Naraoka2017) succeeded in identifying nine amino acids in the Murchison. By analyzing their structural features, the probability of extraterrestrial origin of these amino acids was greatly strengthened. Glycine, alanine, serine, aspartic acid, threonine, valine, glutamic acid, leucine, and isoleucine were among the most important amino acids identified in the Murchison.
The most important signs of extraterrestrial life in the Murchison were related to the research performed by Oba and colleagues (Reference Oba, Takano, Furukawa, Koga, Glavin, Dworkin and Naraoka2022). The results of this research indicated all the building blocks of nucleic acids (adenine, guanine, thymine, cytosine, and uracil) in the Murchison meteorite. It was found that these nucleobases have an extraterrestrial origin, which is a very important subject considering the longer life of this meteorite compared to the planet Earth. This discovery questions many previous hypotheses such as the bubble and fundamental soup model, which emphasize the formation of subunits that make up genetic material and proteins on the planet Earth. All evidence strongly strengthens the feasibility of an extraterrestrial origin of life.
The Murchison meteorite is significant in several respects, two of which are more important than others: The first aspect is the significance of the age of this celestial stone, which is older than the solar system and the Earth (2.5 billion years). Moreover, protosolar grains in this meteorite provide valuable information about the time before the formation of the solar system. The second important aspect of the Murchison is the presence of genetic material components in this 7.5-billion-year-old meteorite. This fact not only strengthens the possibility of the existence of extraterrestrial life in other astronomical objects but also makes the panspermia theory much more successful than in the past.
Allan Hills Meteorite 84001 (ALH 84001)
A group of researchers from the National Science Foundation discovered a meteorite named Allan Hills while traveling by snowmobile in the Allan Hills Icefield at the eastern end of the Transantarctic Mountain Range (Figure 5). In the first descriptions, the researchers described it as a non-chondritic meteorite with a strong matrix fracture, gray-green and with a surface crust consisting of 90% melt. After nine years, an oxygen isotope analysis showed that it is a Martian meteorite that is about 4.5 billion years old and formed shortly after the birth of the planet Mars. Further analysis revealed that this meteorite probably separated from the planet Mars about 17 million years ago from a crater-like area called Valles Marineris and hit the Earth about 13 thousand years ago. The 1.94 kg meteorite is a silicate-rich volcanic rock composed mainly of the following materials: Orthopyroxene (Mg,Fe)SiO3 and with smaller amounts of chromite (FeCr2O4), olivine ((MgFe)2[SiO4]), pyrite (FeS2), apatite (Ca3(PO4)2), and silica-rich glass (Friedmann et al., Reference Friedmann, Wierzchos, Ascaso and Winklhofer2001).

Figure 5. Allan Hills meteorite 84001 (ALH 84001).
The presence of 3.9-billion-year-old secondary carbonate (Figure 6) (which forms at the temperature of the growth range of bacteria) and magnetite in the cracks of this meteorite can be grouped as a weak biological sign (Figure 7). This weakness is because these compounds are formed in both biological and mineral processes. However, the observation of structures resembling microscopic magnetite crystals (as found in magnetotactic bacteria on Earth) in the cracks of this meteorite has made the probability of microbial life on Mars a hot topic (Friedmann et al., Reference Friedmann, Wierzchos, Ascaso and Winklhofer2001). The observation of particles resembling bacterial microfossils in this Martian rock has further strengthened the hope for life on Mars. This is because some of these particles are very similar to the structure of bacterial fossils on Earth, especially magnetotactic bacteria. This claim has always been confronted with the question that “Similarity of the appearance of the discovered particles to the microorganism fossils in the Allan Hills meteoritecannot be a convincing reason” to confirm the presence of microorganisms when this rock was separated from the parent planet. This is because many structures that resemble microfossils can form through mineral processes. The formation of such structures requires water and a temperature in the life-friendly range, suggesting the water flows and a temperature range reasonably similar to that of the Earth life during the formation of these structures (Friedmann et al., Reference Friedmann, Wierzchos, Ascaso and Winklhofer2001).

Figure 6. As a main component in the meteorite ALH 84001, carbonate formation process has been simulated by researchers.

Figure 7. Tubular, elliptical, and rope-like structures of carbonate with a diameter of 20–100 billionths of a meter, have been observed by an electron microscope in a part of the Martian meteorite ALH 84001, and are very similar to bacterial microfossils (https://www.nasa.gov).
Last point on extraterrestrial life giving the meteorite-derived information
General studies on those three meteorites (Orgueil, Murchison, Allan Hills) have revealed thought-provoking facts about life in other parts of the universe. In all three meteorites, there is evidence for microfossils, suggesting the existence of microbial life in the very distant past. Even if one accepts the uncertainty about the origin of fossil-like forms in inorganic and non-biological processes, the presence of DNA/RNA subunits in the Orgueil and Murchison meteorites is reliable evidence for the conditions needed for the formation of nucleic acids in celestial bodies other than Earth. Given the different times and places for the meteorites and the simultaneous discovery of common life features in each of them, it can be concluded that the information obtained from these meteorites is one of the most reliable documents for the high probability of extraterrestrial life. This information proves the most important subunits of biological structures can form in other parts of space. However, a quantitative and qualitative understanding of this topic will require more extensive research using advanced technologies in the future.
Photos and information received by space probes
From Luna-1 to recent probes
Space probes are unmanned devices that leave the Earth’s gravitational field and are sent into space to explore various celestial bodies. These probes carry out a variety of missions far from Earth’s orbit. From their landing site, these kinds of devices can send valuable information to us that cannot be accurately estimated from the ground (Girija, Reference Girija2023). The first space probe was Luna-1 (Figure 8), which was launched by the Soviet Union on January 2, 1959. After this launch, there was close competition between the Eastern and Western blocks for the pioneering role in the space industry and research until the end of the 1980s. Although the Soviet Union had the upper hand at the beginning of this competition, it lost its active role at the end of the 1980s, when it was approaching collapse. Thus, the United States, as the flagship of space exploration, together with the European Space Agency, Japan, China, and India, continued on the path of exploring human civilization in deep space (Petrescu, Reference Petrescu2019). Since the beginning of space search with the help of probes, it has been possible to penetrate the atmospheres of Venus, Mars, Jupiter, and Saturn’s moon Titan over the past six decades (Girija, Reference Girija2023). In the meantime, some probe reports have contained promising information about the presence of signs of life or predisposing factors for the emergence of life in the atmospheres of these planets.

Figure 8. Luna-1 at the memorial museum of astronautics.
The below reportshave strengthened the emergence of life forms even at the microbial level: water on Mars by the Curiosity and Phoenixrovers (Petrescu, Reference Petrescu2018), liquid water streams by the Spirit and Opportunity rovers(Gargaud et al., Reference Gargaud, Irvine, Amils, Claeys, Cleaves, Gerin, Rouan, Spohn, Tirard and Viso2015) (Figure 9), frozen water on Mars by the Phoenix rover (Figure 10), huge glaciers on Saturn’s moon Enceladus by the Cassini rover (Dong et al., Reference Dong, Hill and Ye2015) (Figure 11), volcanic activity on Jupiter’s moon by the Voyager 1 rover (Davies and Hargitai, Reference Davies and Hargitai2014) (Figure 12) and frozen water at Mercury’s north pole by the Messenger rover (Johnson and Hauck, Reference Johnson and Hauck2016) (Figure 13).

Figure 9. Thick layers of sedimentary rocks on the lake bed and edge in the Gal Mars crater. Recorded by the Curiosity spacecraft (NASA/JPL).

Figure 10. Ice at a depth of 3 cm in the North Pole of Mars. Recorded by the Phoenix spacecraft (NASA/JPL).

Figure 11. The geysers of the Antarctic Enceladus, which are thrown to higher altitudes in the form of ice masses. Recorded by the Cassini rover (NASA/JPL).

Figure 12. Two simultaneous volcanic eruptions on Jupiter’s moon Io, where volcanic gray clouds rise 150 miles high. Recorded by the Voyager space probe (NASA/JPL).

Figure 13. Regions near the poles of Mercury that are constantly in shadow and are cold enough to store water as ice. Recorded by the MESSENGER rover from the NASA Applied Physics Laboratory/Johns Hopkins University, Carnegie Institution of Washington (Armytage et al., Reference Armytage, Anzures, Banerdt, Benkhoffet, Besse, Blewett, Bott, Byrne, Carli, Chabot, Chapman, Cloutis, Cremonese, Denevi, Deutsch, Dong, Doressoundiram, Dygert, Ebel, Ernst, Fassett, Genova, Grava, Hauck, Heyner, Hussmann, Iess, Imber, Izawa, Izenberg, James, Kerber, Kinczyk, King, Klima, Klimczak, Knibbe, Lawrence, Lucchetti, Mazarico, McCubbin, McNutt, Nittler, Oberst, Ostrach, Padovan, Pajola, Panning, Parman, Peplowski, Prem, Roberts, Schmude, Schorghofer, Slade, Stark, Strauss, Susorney, Thompson, Udry, Vander Kaaden, Vervack, Varatharajan, Weider, Whitten, Wilbur and Williams2018).
Last point on extraterrestrial life giving the rover-derived information
Evidence on the existence of water or environments resembling the ecosystems on Earth (such as volcanoes, ice, oceans, and water currents) in some planets has paved the way for the next steps in the search for life. Up to this point, they have not yet been confirmed as definitive proof of the life on the planets and celestial bodies studied. Apart from this problem, there are other challenges on the path of research with rovers. Even if there is life on some planets, the organisms living there might have different biochemistry to terrestrial life and use various biochemical patterns/pathways to obtain energy, reproduce, and form their structures (Hargitai and Kereszturi, Reference Hargitai and Kereszturi2015). Assuming that there are life forms whose biochemistry is similar to terrestrial organisms, proving the existence of such organisms is very difficult and requires a comprehensive study of samples taken from probes from different planets. This is because, despite the great biodiversity on Earth, more than 99% of microorganisms on this planet are still unknown. Moreover, due to the lack of knowledge about the growth requirements of these microorganisms, it is not possible to prepare the culture medium for the study of their structural and metabolic properties (Bodor et al., Reference Bodor, Bounedjoum, Vincze, Erdeiné Kis, Laczi, Bende, Szilágyi, Kovács, Perei and Rákhely2020; Pourmazaheri and Tabatabaei, Reference Pourmazaheri and Tabatabaei2013). Even if there is microbial life that has a similar biochemical pattern to terrestrial life, the possibility of identifying these organisms requires extensive research into the metabolic needs and growth conditions to create a suitable environment for their cultivation (Seyedpour Layalestani et al., Reference Seyedpour Layalestani, Shavandi, Haddadi, Amoozegar and Dastgheib2021). The knowledge obtained from the space probes will therefore enable researchers to know the basic conditions for life (Billings, Reference Billings2012).
Radio telescope observations of cosmic objects to search for life
Radio telescope for exploring organic molecules
Radio telescopes are the latest tools that have significantly contributed to the advancement of human knowledge in space science. These telescopes are huge radio antennas consisting of one or more large dishes. Unlike optical telescopes, they receive process, and record radio waves (with a lower frequency and longer wavelength than visible light) on a large scale. The processing of radio waves received from different parts of the universe provides researchers with valuable information about the quantity and quality of celestial phenomena (Song, Reference Song2023) (Figure 14).

Figure 14. Radio telescope ALMA. This radio telescope is located in the Atacama Desert of Chile and it is one of the most important human tools to prove the existence of amino acids and other precursor molecules for life. Since 2006, this device has been used to observe the formation regions of stars and planets.
Nowadays, astronomers can use radio telescopes and radio spectrometers to check the abundance of various chemical substances in cosmic phenomena, such as cosmic clouds. One of the most interesting findings discovered by modern radio astronomy is the detection of organic molecules in dust and giant clouds in interstellar space. This exploration has led to the identification of more than 100 important organic molecules that play a role in the origin of life. Among the most important of these molecules are the subunits of nucleic acids (including adenine, guanine, thymine, cytosine, uracil, niacin, and xanthine), some of the subunits of proteins (such as the amino acid glycine) (Figure 15). These organic molecules were found mainly in the regions where interstellar dust is present in greater abundance, and it seems that these regions are precisely where star formation is most likely to occur (Davies, Reference Davies2018).

Figure 15. Cloud of gas and dust in the constellation Scorpius. This region is where complex molecules can be seen using a radio telescope. In this type of region, new stars are formed from the reservoir of gas and dust in the cosmic clouds. The Scorpius OB star cluster (seen as a cluster of hot stars) is sinking into the cosmic cloud in a reddish glow.
Last point on extraterrestrial life giving the radio telescope-derived information
Although the existence of life in different parts of the universe cannot be definitively confirmed with a radio telescope, a planet or celestial body can be considered a possible candidate for life based on its atmospheric composition and orbital position. Spectroscopy of biological signs only provides information about life indicators in heavenly bodies to researchers. For example, the simultaneous presence of oxygen and methane in the atmosphere of a celestial body can be deduced as a strong indicator of biological processes (Atri et al., Reference Atri, DeMarines and Haqq-Misra2011). Various types of constituent subunits of nucleic acids and amino acids by radiotelescopes greatly strengthen the panspermia theory. Like the information received from meteorites, these results also confirm the entity of building blocks of nucleic acids and proteins in heavenly bodies. Full confirmation of this theory requires more extensive research due to the lack of sufficient documentation (Vakochand Harrison, Reference Vakoch and Harrison2022). Given different conditions on various planets, the proof of life-forming components in stellar objects in turn strengthens the possibility of extraterrestrial life.
Claims and documents focused on extraterrestrial intelligence and civilization
Since the beginning of history and even prehistoric times, many claims have been made about extraterrestrial civilizations that mostly lack credibility for scientific reference. Despite the observations and recording of UFO images, many of these reports have been emotional and exaggerated even in modern times. It seems that the desire to search for life outside the Earth and in other heavenly bodies has always been beyond the thirst for knowledge. Various reasons can be inferred to justify the unusual enthusiasm of mankind for extraterrestrial civilizations. These reasons have been different depending on various historical, cultural, geographical, and social periods (Davies, Reference Davies2018). However, all of these arguments can be seen as arising from common roots, which are:
First: Dealing with strange and wonderful phenomena and creatures that have greatness and power greater than the human species has been attractive in most cases. This type of attitude, which prevailed mainly in the pre-Renaissance and Industrial Revolution eras, considered connecting with superhuman forces and beings as the key to achieving many of one’s imaginations and desires (Michaud, Reference Michaud2007). These thoughts have even continued in the form of some beliefs based on pseudo-science until modern times. Especially in some pseudo-scientific beliefs originating from the Conspiracy theory, extraterrestrial beings (reptilians or humanoid reptiles) are given to be the beings controlling the Earth. These beliefs are not scientifically confirmed (Paura, Reference Paura2017).
Second: Avoiding the fear of being alone in the vast expanse of the universe can be another reason for human interest in alien civilizations. Since the gradual emergence of Homo sapiens during evolution, there has always been a desire to find fellow humans and avoid loneliness in this species. In many cases, finding conspecifics or even other living beings has led to unpleasant conflicts such as exploitation and colonization, but the discovery and communication with other cultures and civilizations have caused tremendous progress in science and technology. Therefore, the desire to find other civilizations has always been greater than the fear of encountering invasive alien species (Bennett, Reference Bennett2011). To better understand this case, imagine a small sailing boat in the heart of a huge ocean with invisible shores on a cloudy and mysterious night. In such a situation, seeing any light beam from far away can be encouraging for the passengers and reduce the bad feeling of being alone. Although the distant light may be caused by a pirate ship’s deck light, the fear is no more frightening than the confusion caused by absolute solitude in a vast ocean.
Third: The desire to use advanced science in the case of communication with a non-hostile alien civilization can be another reason for the human desire to find and communicate with alien extraterrestrial intelligence. This type of thinking has been of interest among some people from the higher spectrums of human society, especially among politicians and science/technology experts in nonpublic circles. There is also an opposite view, according to which contact with a hostile advanced extraterrestrial civilization can not only have devastating results but can also lead to human extinction. Therefore, some space science experts suggest to stop investing in projects related to contact with extraterrestrial intelligence. However, it is not far off to expect that if there is an advanced space civilization, the life and civilization existing on planet Earth will be discovered by them and have inevitable good or bad consequences (Hatfield and Trueblood, Reference Hatfield and Trueblood2021).
Fourth: The most important reason that flows in the human unconscious is the desire for the survival of the human species. This desire has been shown since the beginning of life by “genes that adapt more to the environment” in living beings. By selecting features based on intelligence and wisdom, the human species has been justified by progress in culture, civilization, and technology. Although these developments are outwardly the processes that have provided more human welfare throughout history, inwardly they have caused more adaptation and help the survival of the human species. The passion to search can be regarded as a type of behavior for adaptation during natural selection and in the direction of genome survival (Kidd and Hayden, Reference Kidd and Hayden2015). It seems this enthusiasm is an attempt to increase the human habitable space in the universe, which in turn can raise the survival chance of the human genome. With a glance at the huge amount of recent investment in space science, it is speculated that these projects cannot be just a result of some kind of superficial curiosity, but are rooted in more important issues that are directly related to Human survival.
Given the above arguments about the reasons for the importance of extraterrestrial and intelligent civilizations for humans, the ability of this species to record documents from ancient times will not be out of mind. With the emergence of civilization, the invention of the alphabet, and the expansion of the printing industry, the recording of human events/observations gradually became much faster than in the past. Most of the historians, artists, writers and publishers of the pre-modern eras were dependent on the government and at the disposal of their interests (Dominik and Zarnecki, Reference Dominik and Zarnecki2011). Thusit is not possible to completely rely on them without taking into account all the documents and writings of their contemporaries. In the meantime, with the invention of the camera in the 19th century and the development of optical recording tools, the process of recording events expanded more reliably. From the second half of the 20th century and simultaneously with the third wave of human civilization (Huntington, Reference Huntington1991), advanced communication technologies could increase the speed and accuracy of recording observations.
Other documents and observations
In the last few decades, the verification of images/documents obtained from unidentified flying objects (UFOs) and also scientific-artistic-historical works has been done more carefully. Today, space science researchers use advanced computer processors to analyze various documents from a range of information sources to explore extraterrestrial intelligence. The following are among the most important sources of such knowledge: (1) The sighting of UFOs and aliens; (2) The search for extraterrestrial intelligence (SETI) by trying to send messages to aliens; (3) Historical, scientific, cultural, and artistic writings related to the past era.
Information about the sighting of UFOs and aliens
In general, the term UFO does not necessarily mean aliens but refers to any flying object whose nature is not easily recognizable. The initial definition of the UFOs was based on military logic. In this case, any aerial object that is unusual in terms of its aerodynamic characteristics and performance and does not resemble any type of airplane, rocket, or other man-made flying device can be grouped as a UFO. From the perspective of space science, UFOs are luminous objects in the sky or on the ground that not only lack conventional behavior and appearance for observers, but their nature and origin remain unknown after careful examination by experts (Zhang et al., Reference Zhang, Yang, Luo and Fan2023).
In many cases, UFO sightings from close distances have been reported by pilots, and these special cases are called contact experiences. Since these experiences occur suddenly and are not repeatable, they lack a methodological standard for scientific confirmation. To classify individual experiences of UFO sightings, various classification systems have been invented. One of the most common of these systems is Alan Hendry’s (Reference Hendry1979) classification. Based on this system, man-made aircraft are divided into different types such as identified flying objects, deceptive flying objects, fantasy flying objects, and exceptional flying objects. If aUFO is not among those objects, it can be placed in four groups: Close to detected flying objects; Questionable UFO (Although this group has a high level of wonder, conventional explanations about their terrestriality cannot be completely rejected); A good UFO (which has several amazing features that are at least justifiable); The best UFO (which has a very high degree of wonder and strangeness that cannot be justified by conventional explanations) (Zhang et al., Reference Zhang, Yang, Luo and Fan2023).
According to the definition and classification of UFOs, reports based on their observations can be classified into two categories: reports from individual observations and ones from scientific studies.
Review the history of individual UFO sightings
Since the end of the 19th century, several times a wave of reports about the sighting of UFOs have become the headlines of the world’s news media. The first wave was between 1896 and 1897 in the United States, several years before the invention of the first airplane. Given the industry of man-made airships for traveling long distances at that time, today it seems that these observations were related to these airships, which were still considered a strange phenomenon for many people due to their newness. The next wave of UFO sightings was reported in 1944 in the final months of World War II by pilots in the skies over Europe. At first, due to their distinctive appearance and maneuvers, American pilots speculated them as German secret weapons. In some other reports, German pilots also observed them and were unable to explain them. This wave of reports is justified to a large extent considering Nazi Germany’s pioneering in the aviation industry and the use of the most pioneering weapons of its time (such as fighters equipped with jet engines) in the final months of the war. The next wave of UFO sightings occurred shortly after the end of World War II in Scandinavia when there were numerous reports of wingless flying objects with a rocket-like appearance, which at that time were called ghosts. Given the expansion of the ballistic missile industry after World War II and using the technology of the defeated Germany, these objects can only be the missile tests of the Soviet Union. The new wave of UFO sightings began in 1947 and was recorded and disseminated by various people due to the expansion of recording tools. From the second half of the 20th century onwards, the sighting of UFOs became particularly important in intelligence and military institutions in the United States and many other countries. The security and military risks of these objects were evaluated and their study took a scientific form (Zhang et al., Reference Zhang, Yang, Luo and Fan2023). In recent years, many files related to UFOs have been made available to the public on the Internet.
Review the history of scientific UFO sightings
Due to its military importance and public/social interest, the first government scientific investigation into UFOs was conducted in the United States in 1952 under the Project Blue Book. The result of this research indicated that there is no security threat from UFOs and the lack of evidence to consider these objects as extraterrestrial. From 1952 to 1954, about 3 thousand UFOs were reported, and after statistical analysis, 35% of all observations remained unexplained. In the United Kingdom, a UFO working group was launched that was affiliated with the British Ministry of Defense. This group was the first official institution for the study of UFOs, it began its activities in the early 1950s. This institute conducted its research by reviewing hundreds of reports of UFO sightings, and finally concluded that it is not possible to scientifically confirm or reject the extraterrestrial nature of UFOs (Davies, Reference Davies2018).
The most comprehensive scientific study of UFOs has been done under Condon’s report, which was carried out from 1966 to 1968 by a research team led by quantum physicist Edward U. Condon at the University of Colorado. This 1,000-page report, which was the result of a detailed review of documents obtained from several projects using scientific principles, indicated that there is no scientific explanation for some sightings of UFOs. The overall conclusion showed that 21 years of studying UFOs in different projects did not have any results for the advancement of human scientific knowledge. Although Condon’s report seems disappointing to a large extent, the research facilities and technology of the 1960s should be kept in mind, which were significantly lower than today. After that, many UFO research institutes were formed in Europe and other parts of the world such as MUFON-CES. Until today, these institutions continue to work together with the American NASA administration and study the reports of UFO sighting and their effects (such as radar observations, changes in the land surface and vegetation, functional disorders of vehicles, and gravitational and functional effects) (Zhang et al., Reference Zhang, Yang, Luo and Fan2023).
Studies show that a small percentage of UFO reports remain unexplained, and in most cases, these phenomena are related to man-made aircraft. In the meantime, flying luminous balls (which are mainly seen at heights close to airplanes and near the surface of the earth) as well as moving luminous forms (which are sometimes reported at high altitudes and in the thermosphere layer) are controversial and unexplained cases until recent years. These two phenomena have been seen and recorded many times in the sky of different parts of the world. The latest research has largely revealed the ambiguity of these two mysterious phenomena. Nevertheless, the flying luminous balls in the lower layers and the moving luminous forms in the upper altitudes of the Earth’s atmosphere require much more extensive research (Davies, Reference Davies2018).
Flying Luminous Balls in the lower layers of the atmosphere and the range of the flight altitude of airplanes
Flying luminous balls are another unknown phenomenon and mainly are among the UFOs with unusual behaviors. For example, they fly at a speed faster than the plane, they come close to the plane and disrupt its electronic system, emit a luminous cone, and become invisible on the radar screen. From one of the most important assumptions that can be justified with more scientific arguments, these unusual luminous balls (which range from a few microns to tens of meters in diameter) are most likely a type of ball lightning that is covered in water molecules. It seems that the rays are a result of synchrotron radiation of electrons that are part of the nucleus of the lightning ball. The reason for the supersonic speed of these lightning balls can most likely be due to the release of small charged water clusters from their surface. The questionable point about these glowing spheres is how a large amount of energy can be placed inside a shell of polarized water. This issue can be justified with various arguments (Vakoch and Harrison, Reference Vakoch and Harrison2022).
Some scientists believe that the attraction created between electrons due to the accumulation of kinetic energy by protons around them is the possible reason for this phenomenon. Others believe that the magnetic field created by the eddy electric current inside the lightning balls can cause the coherence of the high level of energy inside the ball. Some others believe that hot steam and chemical energy caused by oxidation can be the cause of this phenomenon. One of the most reasonable models is presented by Sapogin. From this model, spherical layers with similar charges create a type of charge, which in turn creates a type of self-generating electric field that causes the lightning balls to coalesce. The reason for the creation of a lightning ball can be any of the above cases, but it seems that certainty in this case requires wider research (Nikitin, Reference Nikitin2024).
Moving luminous forms in the upper atmosphere
Self-illuminated plasmas are among the strangest UFOs that have been seen at altitudes 200 miles above the Earth’s surface in the form of cones, clouds, donuts, and cylindrical spheres. These objects sometimes disturb the electromagnetic activity of the satellite and sometimes they come close to the space shuttles (Figures 16 and 17). Computer analysis also indicates different speeds and even stationary states, change of direction at any angles, accumulation, engaging, “predatory hunter” behavior, and a trail of plasma dust along the movement path of these bright plasma masses (with a diameter of about one kilometer). These observations have been seen many times by astronauts and pilots. Although this phenomenon is not organic and the nature of these luminous masses remains elusive, plasma dust and plasma behave like multicellular organisms. Plasma has an inorganic nature and consists of ionized gases. The observation of the luminous plasma phenomenon in the thermosphere layer and their absorption towards electromagnetic energy sources tells the self-propagation mode that exists in the organic organisms of the Earth. These observations in the plasma dust are not only an amazing phenomenon but also strengthen the existence of inorganic life in the upper layers of the atmosphere. A sign of the extraterrestrial nature of these phenomena is not yet available, but these inorganic masses show behaviors similar to organic living organisms (self-reproduction, self-sustainability and following the laws of natural selection) (Joseph et al., Reference Joseph, Impey, Planchon, Del Gaudio, Safa, Sumanarathna, Ansbro, Duvall, Bianciardi, Gibson and Schild2024).

Figure 16. Plasma phenomenon with an approximate diameter of one kilometer in the thermosphere layer (Joseph et al., Reference Joseph, Impey, Planchon, Del Gaudio, Safa, Sumanarathna, Ansbro, Duvall, Bianciardi, Gibson and Schild2024).

Figure 17. Four luminous halo-like objects over Salem Air Force Base, Salem, Massachusetts on July 166, 1952, recorded by a member of the Coast Guard. At the time of photography, the weather was reportedly cloudy and prone to thunderstorms (Joseph et al., Reference Joseph, Impey, Planchon, Del Gaudio, Safa, Sumanarathna, Ansbro, Duvall, Bianciardi, Gibson and Schild2024).
Claims related to the sighting of extraterrestrial beings or their bodies
There were so many unsubstantiated claims about alien sightings by various people in the 20th and 21st centuries that. These claims not only became the main subject of many novels, films, and television shows in the contemporary era, but they also caused the creation of some sects under the influence of assumptions based on pseudo-science and Conspiracy theories. To attract more audiences, some media also created a nonscientific intellectual atmosphere and a sentimental attitude towards this issue by promoting such claims. In these cases, different appearances such as green-headed dwarfs, gray humanoid aliens, humanoid reptiles, and other types of unconventional morphologies were attributed to these alien creatures (Vakoch and Harrison, Reference Vakoch and Harrison2022). Even some conspiracy theorists like David Icke went further and claimed that some humanoid reptiles with the ability to transform into humans are controlling society and have formed some very famous political and artistic faces. The big fans of these lines of thought always accused governments and international structures of secrecy regarding foreigners. However, claims arising from pseudo-scientific thinking and conspiracy theories lack scientific validity and cannot be cited in any way (Mahl et al., Reference Mahl, Zeng and Schäfer2021).
The most important claim of alien sightings was made in September 2023 by the presentation of two different human-like corpses, 60 and 75 cm long, from Peru, a thousand years ago, to the Congress of Mexico. These corpses were exhibited by Jaime Maussan one of the famous journalists and media figures in Mexico. In this meeting, which was held in the presence of high-ranking Mexican political officials, claims were made about the genetic difference (of more than 30%) with the human species, the presence of osmium and cadmium metal chips, and the embryonated eggs in one of the corpses. The raising of such a claim in the highest political circle in one of the influential countries of Latin America was initially accompanied by a lot of media controversy (Vakoch and Harrison, Reference Vakoch and Harrison2022).
But a little later, the investigation of the Mexican prosecutor’s office denied that these bodies were extraterrestrial and stated that these corpses were artificial. Academic societies also refused to confirm the extraterrestrial nature of these bodies. They stated that evidence was not enough to confirm the extraterrestriality of those corpses. In addition to rejecting the extraterrestrial nature of these bodies, the Peruvian Ministry of Culture filed a complaint against Maussan and the people involved in this claim. Paying attention to the fact that until this moment, no valid scientific article/report has been presented regarding the authenticity of this claim, so its authenticity cannot be confirmed (Bennett, Reference Bennett2011). It seems that this event was only a demonstration to promote some nonscientific goals in the media space (Figure 18).

Figure 18. The bodies claimed to be space aliens in the Mexican Congress by Jaime Maussan, one of the prominent and controversial media figures of Euronews.
Last point on extraterrestrial life giving the UFO/alien-derived information
Although some reports of UFOs remain ambiguous, there is still no reliable evidence that these phenomena are extraterrestrial. There has always been some sentimentality about the extraterrestrial nature of UFOs. The latest NASA comprehensive research report in September 2023 also confirms this conclusion. According to this report, which was announced by NASA after a year of the research group’s activity, it is stated that despite the uncertainty of the origin of some unidentified flying objects, there is no reliable evidence that UFOs are extraterrestrial. Proving such a matter will require more advanced technology and most likely the use of artificial intelligence in the future (https://parsi.euronews.com).
Apart from a series of unsubstantiated claims (Mahl et al., Reference Mahl, Zeng and Schäfer2021), and the rejection of alleged extraterrestrial bodies by the Mexican Congress, there is no credible scientific evidence of direct sightings of intelligent extraterrestrials to date. However, the possibility of such creatures cannot be ruled out. Although the sighting of extraterrestrial intelligent beings cannot be confirmed by scientific standards, it is not possible to reject such beings with current technology.
Search for extraterrestrial intelligence by attempting to send messages to the universe
Another attempt to discover extraterrestrial intelligence, which was proposed in the 1960s, is to send an electromagnetic signal to different parts of the universe so that if there is an intelligent civilization in other parts of space, alien intelligence will receive this message. In the physical method, it is used to send physical artifacts containing messages (such as Voyager’s golden records) into space. In the method of sending electromagnetic signals, radio waves or laser pulses are sent to the ends of the universe using large radio telescopes. So if there is intelligent life near the civilization of the Earth, they will find out about their intelligent neighbors on Earth (Figures 19 and 20).

Figure 19. Arecibo observatory with a width of 305 meters located in Puerto Rico. This observatory is used as the world’s largest and most sensitive radio telescope in the search for extraterrestrial intelligence (www.scientificamerican.com).

Figure 20. Voyager spacecraft and discs containing sounds and images related to Earth and terrestrial life including things like the sound of water, animals, pieces of music from different cultures, the word “hello” in different languages, and text from the politicians of the time (https://www.nasa.gov).
The question that is raised about this method is that if such civilizations exist and even if these messages are received, how can they decode the received waves? Another question is that if these messages are received by hostile civilizations, they may become aware of the location of the Earth, and thereby it has potential dangers for our civilization. Decisions about the use of these methods are made only by a few people in human society, and there is no kind of survey about this from the general public. Therefore, in terms of legitimacy, it lacks sufficient credibility and is undemocratic (Hatfield and Trueblood., Reference Hatfield and Trueblood2021). No sign of receiving the message has been obtained, so the efficiency of these methods is also questioned by many experts. Supposedly, even if radio messages are received and decoded by a possible civilization, their distance may be so great that it takes centuries, millennia, or more time to receive and react to these messages (Atri et al., Reference Atri, DeMarines and Haqq-Misra2011). It can be concluded that until this moment, the reports of the SETI using physical and radio messages have not had any results.
Historical, scientific, cultural and artistic works related to past eras
One of the hypotheses that has been somewhat popular in the contemporary era is the connection of space aliens with human ancestors in ancient times and the creation of changes in the human species. One of the first important works mentioned in this matter is Däniken’sChariots of the Gods, which was published in 1968. In this book, by relating some huge ancient structures and paintings to extraterrestrial intelligent beings, the author has claimed the connection between space aliens and ancient humans. According to the author, these communications have caused great changes in the evolution of human society. Such statements provoked criticism of his book in some scientific and ideological circles (Rayhan, Reference Rayhan2023). But Daniken’s claims were not the end of this story, and for years after the publication of this book, fans of Daniken’s line of thought and also the media attributed the discovery of every extraordinary ancient phenomenon to intelligent space aliens. Among the most important of these phenomena, the following can be mentioned: Egyptian pyramids, the Nazca lines in Peru, thepyramids/monuments of the City of Gods (Teotihuacán) located in Mexico, Saxe-Human temples, Cusco monuments in Peru, Stone structures/elevations of Stonehenge, Colossal Moai statue located on East Polynesian Easter Island, and even some petroglyphs with a design similar to ancient astronauts (Figure 21).

Figure 21. (a) Egyptian pyramids; (b) Topography of Nazca in Peru; (c) The monuments of the City of Gods (Teotihuacán) in Mexico, (d) Stonehenge rock formations in England; (e) the huge Moai statues located in East Polynesian Easter Island; (f) Paintings with a design similar to ancient astronauts in Valcomonica, Italy.
Since 2010, the media environment, especially in the United States, suddenly faced a huge wave of programs based on pseudo-scientific theories. The following topics became hot discussions among viewers and some members of society: (1) the relationship of extraterrestrials with ancient humans, (2) their effects on the development of human technology, and (3) the intervention of aliens in the affairs of human civilization. It seems that due to a kind of media competition to increase the number of audience, some media turned to producing some programs with popular titles. However, these programs lacked sufficient standards and documents to confirm the connection of extraterrestrial intelligence with human ancestors. The media tried to mix scientific and nonscientific content and to blur the line between science and pseudo-science only for audience attraction (Schiele, Reference Schiele2020).
Last point on extraterrestrial life giving the historical/scientific/cultural/artistic-derived information
Attributing some structures and ancient works to extraterrestrial intelligence is caused by a kind of unsubmissive look and ignoring many abilities of ancient people by contemporary man. One of the most impressive of these structures is the Egyptian pyramids, which consist of about 5.5 million tons of limestone, 8 thousand tons of granite, and 500 thousand mortar. In the past, it was thought that most of these materials were transferred from Aswan (located in the south of Egypt) to Giza (in the north of Egypt) from a distance of 800 km (Vakoch and Harrison, Reference Vakoch and Harrison2022).
For many believers in the theory of “the construction of the Egyptian pyramids by extraterrestrial intervention,” it is not possible to transport this volume of thick stones from long distances from the south to the north of Egypt without the use of trucks and modern machines. However, with the use of satellite images, geophysical studies, analysis of deep soil cores, subsurface structures, and sedimentology, this secret has been revealed to a large extent (Ghoneim et al., Reference Ghoneim, Ralph, Onstine, El-Behaedi, El-Qady, Fahil, Hafez, Atya, Ebrahim, Khozym and Fathy2024). Recently, it was found that during the construction of the Great Pyramid and the adjacent pyramids, a large branch of the Nile River passed through the vicinity of the construction site of the pyramids. Instead of rolling the stones or transporting them with timber, the ancient Egyptian workers and engineers took advantage of the flow of water and the use of floats to carry the necessary stones and materials along the Nile River. The pyramids are the biggest examples of amazing human structures.
Another common case regarding the connection between ancient people and intelligent spatial beings is the Nazca Geoglyph. These maps are located in an area of about 450 square kilometers in the southern desert of Peru, which depict creatures such as hummingbirds, spiders, monkeys, lizards, pelicans, trees, plants, flowers, and some geometric shapes. Distortions have often been made in the dimensions of these forms. For example, one of them is the leg of a bird, which some have claimed to be similar to the runway of an airport, while the diameter of this leg is only four meters. On the other hand, the oldest of these maps (made of piled stones) dates back to 500 BC. That is, when the Nazca people were working on an efficient system of transferring water from the depths of the earth to the surface, and had made progress in terms of engineering sciences in ancient times. Therefore, the design of these maps in the framework of ritual and cultural rituals, despite the thought they provoke, is not impossible and can be justified by the technology of the ancient inhabitants of Peru (Jarosz, Reference Jarosz2014).
From evidence obtained from the ability of ancient people to create huge structures and paintings, such as the Egyptian pyramids and the Nazca lines, it is possible to justify the construction of other cases of these ancient wonders (The Saxe-Human temples, the city of Cuzco in Peru, the temple of the Gods of Mexico, the Moai sculptures of Easter Island, and the stone ascents of Stonehenge). In the case of ancient astronauts, according to Carl Sagan (as one of the main experts and researchers in the field of extraterrestrial intelligence), there have always been various types of wrong reports, distortions, and even fraud (Sagan, Reference Sagan1986).
In general, it is impossible to go through all the claims and reports about ancient astronauts and the possible effects of alien intelligence on the human species without thinking. Their confirmation by the scientific community requires extensive verification and investigation. Despite the ambiguities about some of these phenomena, there is still no sufficient scientific evidence to confirm the connection between extraterrestrial civilizations and the ancient ancestors of modern humans. This result does not in any way mean to reject extraterrestrial intelligence, but it refuses to confirm the claims made about the establishment of alien intelligent beings with human ancestors. Unfortunately, the long-term experience of the scientific community in different periods of history indicates that the discovery of any challenging phenomenon was initially accompanied using nonscientific hypotheses by non-experts and government-affiliated intellectuals (Vakoch and Harrison, Reference Vakoch and Harrison2022). However, with the expansion and progress in many fields of technology in the coming years, many mysteries will be revealed.
Possible habitats and life forms on terrestrial and extraterrestrial objects
The probability of encountering intelligent life forms beyond our Solar System is quite low; however, the chance of discovering some forms of alien life, if they exist, is relatively promising. The potential identification of living organisms or evidence of their historical presence on other celestial bodies, theoretical frameworks, lab simulations, and analogous habitats on Earth can enhance our comprehension of the possible manifestations of life, both known and unknown. Table 3 enumerates the types of life that could theoretically thrive under various planetary conditions. This table illustrates that a variety of planetary environments, capable of supporting both known and unknown life forms, are available for astrobiological research (Irwin and Schulze-Makuch, Reference Irwin and Schulze-Makuch2020)
Table 3. Possible habitats and life forms on celestial objects

Abbreviations: AI, Amorphous inorganic; AO, Amorphous organic; MMI, Micro/macro individuals.
The last question: Can Earth’s life grow on extraterrestrial life?
Now we must address some important questions? If the human ancestor has been originated from extraterrestrials, could Earth’s life use extraterrestrial organic carbon at all? If life will find far away and humans can no longer live on Earth, are humans and terrestrial organisms compatible with the organic carbon on those planets and can they use them? Answers to these questions need to explore the ability of extraterrestrial organic carbon to be used by Earth’s living organisms. Researchers demonstrated that organic carbon from meteorites serves as the exclusive carbon source for the growth of anaerobic microorganisms and can be integrated into Earth’s microbial proteins. This was achieved through 13C-stable isotope labeling + infrared spectroscopy, which makes it possible to observe meteorite carbon incorporation into microbial biomass. These new findings indicate that organic carbon present in extraterrestrial environments is accessible to contemporary anaerobic life forms, suggesting that such organic materials could have been available to sustain the earliest heterotrophic organisms prior to the extensive accumulation of biomass on Earth. These results imply that the substantial amounts of organic carbon produced in the early stages of protoplanetary discs may have provided a vital energy source for the development of early planetary biospheres. Furthermore, the biological accessibility of extraterrestrial organic compounds holds significant potential for in situ resource utilization in the future explorations at the space (Waajen et al., Reference Waajen, Lima, Goodacre and Cockell2024). Since microscopic and macroscopic forms of life have the ability to consume a wide variety of carbon compounds and produce a wide range of carbon-containing metabolites, (Karami et al., Reference Karami, Maleki, Karimi, Poormazaheri, Zandi, Davari, Zand Salimi, Gharibi and Kalantar2016; Kalantar et al., Reference Kalantar, Soltani, S, Habibi, Poormazaheri, Marashi, Amini, Afshar, Kabir and Safari2015) the presence of organic compounds, even at the molecular scale, could be very important in the search for extraterrestrial life.
Artificial intelligence and analysis of scientific evidence of extraterrestrial life
So far, all searches and evidence regarding the potential existence of extraterrestrial life have been examined within the framework of human cognitive abilities and analysis. In fact, humanity has examined evidence and signals based on its own definition of life, focusing on those signals that the human nervous system is capable of perceiving and analyzing. However, the vast array of signals and data present in the cosmos is so extensive that it is impossible for humans to detect many of them. Even if received, numerous signals and signs of life may remain hidden or be overlooked due to the limitations of human neural receptors and cognitive abilities. In other words, since human research has focused on current definitions of life and the known biochemical laws, it is not only conceivable but highly probable that different forms of life exist on Earth and elsewhere in the universe, which, due to their distinct structures and biochemical cycles, might be beyond human comprehension based on current definitions (Davies et al., Reference Davies, Benner, Cleland, Lineweaver, McKay and Wolfe-Simon2009). A multitude of hypotheses regarding the existence of unconventional forms of extraterrestrial life, such as life based on non-water solvents (Bains et al., Reference Bains, Petkowski, Zhan and Seager2021; https://arxiv.org/abs/2401.07296), life based on dusty plasma (Goossens, Reference Goossens2003; Tsytovich et al., Reference Tsytovich, Morfill, Fortov, Gusein-Zade, Klumov and Vladimirov2007), and shadow biospheres (Davies et al., Reference Davies, Benner, Cleland, Lineweaver, McKay and Wolfe-Simon2009), have been proposed. If life based on any of these hypotheses exists, the identification and analysis of biological signals and markers across the vast cosmos would be extremely difficult, if not impossible, for the human mind.
In the vastness of the universe, understanding extraterrestrial life requires data from various scientific disciplines. Information about the complexity of molecular evolution, the intricate nature of many cosmic systems, and the potential environments for life scattered across the immense expanse of the universe can pose numerous questions, challenges, and ambiguities for humans. Solving these complexities would be extremely difficult for human intelligence, requiring extensive research and experience over long periods of time. Therefore, to address many of these challenges and ambiguities, which are hidden in the vast data accumulated from cosmic phenomena, a tool with high analytical and modeling capabilities is necessary. This would allow researchers to decipher many of the hidden mysteries of the universe. Today, with the advent of artificial intelligence (AI), the resolution of these complexities has become easier and is expected to accelerate significantly in the near future, with further development and expansion of this technology (https://www.nasa.gov). AI is a set of techniques and algorithms that allow computer systems to perform mental tasks, such as learning, decision-making, and prediction, by emulating human cognitive abilities (Rodriguez et al., Reference Rodriguez, Tejani, Pydipalli and Patel2018). AI can serve as a tool to enhance analytical activities and, in parallel, support human intelligence in solving many of civilization’s challenges. The use of AI in astrobiology can assist in the search for extraterrestrial life and address several challenges related to space colonization. Some of the most significant contributions include:
Analysis of molecular biological markers from space samples
Recently, the use of AI in analyzing biological markers from meteorites has become possible. The high capacity of AI in processing data obtained from gas chromatography, electron impact ionization mass spectrometry (Pyr-GC-EI-MS), and other precise analytical methods has provided valuable information about the distribution of complex organic mixtures in carbonaceous meteorites (Cleaves et al., Reference Cleaves, Hystad, Prabhu, Wonga, Cody, Economon and Hazen2023). A 2023 study by Cleaves et al., which utilized machine learning to analyze chemical data from ancient rocks and meteorites, showed that combining AI and chemical analysis methods such as chromatography and spectroscopy enables researchers to determine the biological or abiological origin of organic compounds. Identifying whether carbon-based compounds in meteorites and other space samples are biological or non-biological has always been a primary challenge for astrobiologists, and AI appears to be the key to addressing this challenge. Additionally, the combined use of AI and chemical analysis will provide answers to many difficult questions in future samples from space probes.
With this approach, it is even possible to detect biological markers of entirely different forms of extraterrestrial life, which might not rely on DNA, amino acids, or other building blocks of life on Earth (shadow biosphere) if such life forms exist (Davies et al., Reference Davies, Benner, Cleland, Lineweaver, McKay and Wolfe-Simon2009). This is because even alternative forms of life with distinct biochemistry require at least a few compounds for storing information, providing energy, and forming structures, which AI can analyze by understanding the distribution of these compounds and identifying meaningful relationships to detect biological markers.
In this study, the composition of polycyclic aromatic hydrocarbons (PAHs) in the Martian meteorite ALH84001 was analyzed and compared with twelve terrestrial samples. These samples included four igneous rocks (Ig-1, Ig-2, Ig-3, Ig-4), four hydrothermal sedimentary and rock samples (Hyd-1, Hyd-2, Hyd-3, Hyd-4), and four samples of biological origin (Bio-1, Bio-2, Bio-3, Bio-4) (Table 4). To compare the presence or absence of polycyclic aromatic hydrocarbons (PAHs) in ALH 84001 and terrestrial analog samples, we used hierarchical clustering along with a Jaccard distance matrix in Python 3 (Figure 22). First, the analyzed parameters were classified using plus (+) and minus (−) signs, which were then converted into binary values (0 and 1). The results revealed significant variations in PAH composition between the evaluated samples. To validate the hierarchical clustering results, we also generated a heatmap representation of the PAH data, which confirmed similar patterns between ALH 84001 and the terrestrial analog samples (Figure 23). Based on hierarchical clustering method 11 diverse clusters were found in compared PAHs for ALH84001 and terrestrial analog samples. This showed that the evaluated samples showed a significant differences in the analyzed.
Table 4. Presence or absence of Polycyclic Aromatic Hydrocarbons (PAHs) in ALH 84001 and terrestrial analog samples

The presence (+) or absence (–) of polycyclic aromatic hydrocarbons (PAHs) in ALH 84001 and 12 terrestrial analog samples. Data is based on qualitative analysis, without quantitative PAH concentration measurements.
Samples include biogenic rock and sediment (Bio-1–Bio-4), igneous rocks (Ig1–Ig4) and hydrothermal deposits (Hyd-1–Hyd-4):
Bio-1: Stromatolite near Zeerust, South Africa (Sklarew* and Bartholomew 1981).
Bio-2: Organic matter in Jurassic carbonates Orbagnoux, French Southern Jura (Thlaijeh et al., Reference Thlaijeh, Lepot, Carpentier, Riboulleau, Duca, Vojkovic, Tewari, Sarazin, Bon, Nuns, Tribovillard and Focsa2024).
Bio-3: Preserved organic matter in a fossil ocean continent transition in the Alps (Mateeva et al., Reference Mateeva, Wolff, Manatschal, Picazo, Kusznir and Wheeler2017).
Bio-4: Jurassic small sedimentary basins exposed in the northwest onshore area of Sri Lanka (Ratnayake and Sampei, Reference Ratnayake and Sampei2015).
Ig-1: Migmatites from Siljan crater, Sweden (Pikovskya et al., Reference Pikovskya, Khlyninaa and Kutcherovb2021).
Ig-2: Granites from Siljan crater, Sweden (Pikovskya et al., Reference Pikovskya, Khlyninaa and Kutcherovb2021).
Ig-3: Pyroxene obtained from the Geological Museum of the University of Hong Kong (Tian et al., Reference Tian, Liu, Hammonds, Wang, Sarre and Cheung2013).
Ig-4: olivine-type silicates obtained from the Geological Museum of the University of Hong Kong (Tian et al., Reference Tian, Liu, Hammonds, Wang, Sarre and Cheung2013).
Hyd-1: Hydrothermal sediments of the South Mid-Atlantic Ridge (Xin et al., Reference Xin, Zhigang, Shuai, Xuebo, Xiaoyuan, Yao, Baoju, Kunbo, Yunchao and Jiang2014).
Hyd-2: Hydrothermal sediments collected from the Logatchev hydrothermal field on the Mid Atlantic Ridge (Li et al., Reference Li, Peng, Zhou, Li, Chen, Wu and Yao2012).
Hyd-3: Hydrothermal sediments collected from the Kairei hydrothermal field on the Central Indian Ridge (Li et al., Reference Li, Peng, Zhou, Li, Chen, Wu and Yao2012).
Hyd-4: Hydrothermal sediments and rocks from South Mid-Atlantic Ridge (Xin et al., Reference Xin, Zhigang, Shuai, Xuebo, Xiaoyuan, Yao, Baoju, Kunbo, Yunchao and Jiang2014).

Figure 22. The variation of PAHs among the evaluated samples. Hierarchical clustering.

Figure 23. The variation of PAHs among the evaluated samples. Heatmap visualization of the investigated data.
The results of this study indicate that the polycyclic aromatic hydrocarbons (PAHs) detected in the ALH84001 meteorite exhibit the greatest similarity to igneous samples (Ig-1–Ig-4), while showing significant differences from both biogenic (Bio-1–Bio-4) and hydrothermal (Hyd-1 to Hyd-4) samples. These findings, supported by Jaccard similarity analysis and hierarchical clustering, strongly suggest that the PAHs in ALH84001 are most likely of abiotic origin.
In 1996, McKay et al. hypothesized that the PAHs in ALH84001 could be remnants of past biological activity on Mars. This claim, one of the most controversial in the field of astrobiology, was based on the structural resemblance of these compounds to biogenic PAHs. However, the findings of this study contradict McKay’s hypothesis, as the PAH composition in ALH84001 aligns more closely with igneous rocks rather than biogenic materials.
Since PAHs can form through non-biological processes, such as thermal and magmatic reactions, these results suggest that the PAHs in ALH84001 are likely products of geochemical abiotic processes rather than biological activity. This study underscores the importance of understanding abiotic mechanisms in the formation of organic compounds on Mars and highlights that the mere presence of PAHs cannot be considered definitive evidence of past life. Further structural, isotopic, and geochemical analyses will be essential for a more comprehensive evaluation.
Decoding complex signals in case of receiving messages from extraterrestrial civilizations
The SETI has so far failed to find evidence of this type of intelligence (Gundu and Charanarur, Reference Gundu and Charanarur2024). This search involves analyzing a vast array of data, primarily in the form of radio signals or other forms of electromagnetic radiation, looking for patterns in the received waves that could be considered signals from extraterrestrial intelligence. However, the immense size of space, the potential diversity of signals, and the limitations in detecting and analyzing these signals make this approach extremely challenging. One of the biggest challenges is filtering out cosmic noise, which can obscure extraterrestrial life signals, if they exist. AI algorithms, with their ability to process large data sets and model complex behaviors, are capable of identifying and eliminating extraneous noise. Furthermore, AI can discover algorithms previously unnoticed by humans, such as identifying complex patterns or signals that might seem random (Durrani, Reference Durrani2024). The most innovative contribution of AI in this field is the improved accuracy in identifying cosmic signals that might have been undetectable in the past.
Analyzing UFO data and examining the possibility of extraterrestrial origins
One of the main challenges in analyzing UFO data is distinguishing between natural phenomena, artificial objects, and the potential existence of extraterrestrial life. AI, using advanced deep learning algorithms, can simulate models of normal and anomalous behaviors of aerial objects in the sky and differentiate these patterns from the received data. One of the most innovative applications of AI in this context is the use of complex neural networks (CNNs) to analyze images and videos of UFOs. These networks can detect even the smallest differences in structure and movement of objects, so that if an object behaves anomalously, it can be automatically flagged (Ayeni, Reference Ayeni2022). This method significantly increases the speed and accuracy of data analysis and ultimately helps identify unusual cases that may indicate extraterrestrial life.
AI also plays a crucial role in filtering and analyzing radar and acoustic data from UFOs. One of the biggest problems in signal analysis is severe interference and noisy data. In this context, AI algorithms, particularly reinforcement learning methods, can train systems to distinguish valid signals from environmental noise (Thornton et al., Reference Thornton, Kozy, Buehrer, Martone and Sherbondy2020). By combining these algorithms with data on the behavior and characteristics of UFO movement, AI can automatically simulate specific behavioral patterns that are more indicative of extraterrestrial nature. This technique helps researchers gain a better understanding of the nature of observed phenomena and ultimately make more informed decisions on whether these phenomena are extraterrestrial or natural.
Assessing the habitability of other planets using available data
AI, due to its high analytical capabilities, can bring revolutionary changes to how we assess the habitability of planets within our solar system and exoplanets. Machine processing algorithms, by analyzing data from space telescopes, can identify the composition of a planet’s atmosphere and detect essential life elements such as oxygen, methane, and water vapor. Furthermore, deep neural networks can analyze temperature, atmospheric pressure, and distance from the parent star, uncovering habitability patterns that were previously hidden from researchers (Charbonneau, Reference Charbonneau2024). With the development of AI models, humanity can now examine the likelihood of life existing in distant corners of the universe with unprecedented accuracy, potentially marking the first steps toward discovering alien ecosystems.
Conclusion
Our investigation focused on the evidence for extraterrestrial life, including biochemical studies of meteorites, atmospheric data obtained by space probes, assessments of celestial bodies’ habitability through radio telescopes, and claims related to extraterrestrial intelligence and civilizations. While some findings support the possibility of extraterrestrial life, others contradict it. Overall, humanity still has a long journey ahead before reaching a definitive conclusion on this matter. However, recent advancements in artificial intelligence have enabled the analysis of scientific data with unprecedented speed, accuracy, and comprehensiveness. From processing complex datasets to identifying hidden patterns in cosmic information, AI has the potential to revolutionize astrobiological research. By leveraging these cutting-edge technologies, future investigations may take significant strides in understanding the origin, evolution, and potential extent of life in the universe.
Funding statement
This research received no specific grant from any funding agency, commercial or not-for-profit sectors.
Competing interests
The authors declare no conflict of interest.





