Coconut oil, extracted from coconut kernels, is a rich source of medium-chain fatty acids (MCFAs), including lauric acid, capric acid and caprylic acid. This experiment aimed to investigate the protective effect of coconut oil against intestinal injury induced by lipopolysaccharide (LPS) challenge in piglets. A total of 24 piglets were used in a 2 × 2 factorial experiment with dietary treatment (3% soybean oil vs 3% coconut oil) and LPS challenge (saline vs LPS). After 28 days of the experiment, piglets were injected intraperitoneally with LPS (100 μg/kg body weight) or saline. Piglets were slaughtered and sampled for testing. Pigs fed coconut oil had higher average daily gain and body weight during the entire study. Supplementation with coconut oil improved intestinal morphology and barrier function, indicated by increased jejunal villus height, as well as enhanced protein expression of ZO-1 and Occuldin. Furthermore, coconut oil supplementation improved plasma antioxidant capacity indicated by enhanced glutathione peroxidase (GSH-Px) activity and decreased malondialdehyde (MDA) concentration. Moreover, Coconut oil ameliorated the LPS-induced release of pro-inflammatory cytokines, as indicated by decreased IL-1β expression in the jejunum. Coconut oil also alleviated the up-regulation of the expression of necroptosis protein receptor-interacting protein kinase 3 (RIPK3) and mixed lineage kinase-like protein (MLKL) in the jejunum of piglets stimulated by LPS. In conclusion, dietary supplementation of coconut oil can improve the growth performance of piglets, and alleviate LPS-induced intestinal injury and inflammation by inhibiting necroptosis signaling pathway.