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Expression analysis of regulatory microRNAs in bovine cumulus oocyte complex and preimplantation embryos

Published online by Cambridge University Press:  11 October 2011

W.S. Abd El Naby ,
T.H. Hagos ,
M.M. Hossain ,
D. Salilew-Wondim ,
A.Y. Gad ,
F. Rings ,
M.U. Cinar ,
E. Tholen ,
C. Looft  and
K. Schellander
...Show all authors
W.S. Abd El Naby
Affiliation:
Institute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Germany.
T.H. Hagos
Affiliation:
Institute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Germany.
M.M. Hossain
Affiliation:
Institute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Germany.
D. Salilew-Wondim
Affiliation:
Institute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Germany.
A.Y. Gad
Affiliation:
Institute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Germany.
F. Rings
Affiliation:
Institute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Germany.
M.U. Cinar
Affiliation:
Institute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Germany.
E. Tholen
Affiliation:
Institute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Germany.
C. Looft
Affiliation:
Institute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Germany.
K. Schellander
Affiliation:
Institute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Germany.
M. Hoelker
Affiliation:
Institute of Animal Science, Department of Animal Breeding and Husbandry, University of Bonn, Germany.
D. Tesfaye*
Affiliation:
Institute of Animal Science, Department of Animal Breeding and Husbandry, Endenicher Allee 15, 53115 Bonn, Germany.
*
All correspondence to: Dawit Tesfaye. Institute of Animal Science, Department of Animal Breeding and Husbandry, Endenicher Allee 15, 53115 Bonn, Germany. Tel: +49 228 732286. Fax: +49 228 732284. e-mail: tesfaye@itw.uni-bonn.de
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Summary

MicroRNAs (miRNAs) are small endogenous molecules that are involved in a diverse of cellular process. However, little is known about their abundance in bovine oocytes and their surrounding cumulus cells during oocyte development. To elucidate this situation, we investigated the relative expression pattern of sets of miRNAs between bovine oocyte and the surrounding cumulus cells during in vitro maturation using miRNA polymerase chain reaction (PCR) array. Results revealed that a total of 47 and 51 miRNAs were highly abundant in immature and matured oocytes, respectively, compared with their surrounding cumulus cells. Furthermore, expression analysis of six miRNAs enriched in oocyte miR-205, miR-150, miR-122, miR-96, miR-146a and miR-146b-5p at different maturation times showed a dramatic decrease in abundance from 0 h to 22 h of maturation. The expression of the same miRNAs in preimplantation stage embryos was found to be highly abundant in early stages of embryo development and decreased after the 8-cell stage to the blastocyst stage following a typical maternal transcript profile. Similar results were obtained by localization of miR-205 in preimplantation stage embryos, in which signals were higher up to the 4-cell stage and reduced thereafter. miR-205 and miR-210 were localized in situ in ovarian follicles and revealed a spatio-temporal expression during follicular development. Interestingly, the presence or absence of oocytes or cumulus cells during maturation was found to affect the expression of miRNAs in each of the two cell types. Hence, our results showed the presence of distinct sets of miRNAs in oocytes or cumulus cells and the presence of their dynamic degradation during bovine oocyte maturation.

Keywords

Cumulus–oocyte complexEmbryosMaturationmiRNA
Type
Research Article
Information
Zygote , Volume 21 , Issue 1 , February 2013 , pp. 31 - 51
Copyright
Copyright © Cambridge University Press 2011

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