Hostname: page-component-7f64f4797f-g5lq8 Total loading time: 0.001 Render date: 2025-11-03T23:43:58.380Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of low-density lipoproteins extracted from different avian yolks on boar spermatozoa quality following freezing–thawing

Published online by Cambridge University Press:  30 November 2012

Peng Wang ,
Yan-Feng Wang ,
Chun-Wei Wang ,
Shu-Hai Bu ,
Jian-Hong Hu ,
Qing-Wang Li ,
Wei-Jun Pang  and
Gong-She Yang
Peng Wang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Yan-Feng Wang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Chun-Wei Wang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Shu-Hai Bu
Affiliation:
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Jian-Hong Hu*
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Qing-Wang Li*
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Wei-Jun Pang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Gong-She Yang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
*
All correspondence to: Jian-Hong Hu or Qing-Wang Li. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China. Tel: +86 29 87092102. Fax: +86 29 87092164. e-mail: hjh19732008@126.com or liqingwangysu@yahoo.com.cn
All correspondence to: Jian-Hong Hu or Qing-Wang Li. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China. Tel: +86 29 87092102. Fax: +86 29 87092164. e-mail: hjh19732008@126.com or liqingwangysu@yahoo.com.cn
Get access Rights & Permissions [Opens in a new window]

Summary

Low-density lipoproteins (LDL) is known to protect boar sperm during freezing–thawing, but little information is known about the effects of LDL extracted from different avian egg yolks on post-thaw boar semen quality. The purpose of this study was to compare and analyze the effects of LDL at various concentrations and different species on boar sperm quality after freezing–thawing. LDL extracted from the yolk of hen egg, duck egg, quail egg, pigeon egg or ostrich egg was added to the extender at the concentrations of 0.06, 0.07, 0.08, 0.09 and 0.1 g/ml, respectively, and their effects on frozen–thawed boar sperm quality were assessed. According to all measured parameters, the results showed that sperm motility, acrosome integrity and plasma membrane integrity were 43.20%, 52.57% and 48.13%, respectively, after being frozen–thawed with 0.09 g/ml LDL extracted from pigeon egg yolk. All these quality parameters were higher than that of other groups (P < 0.05). In conclusion, our results confirmed that LDL extracted from pigeon egg yolk had the best cryoprotective effects on frozen–thawed boar sperm among all of the groups supplemented with LDL from five kinds of avian egg in extender. The optimum concentration of LDL extracted from pigeon egg in boar semen freezing extender was 0.09 g/ml.

Keywords

Avian egg yolk LDLBoar semenCryopreservationSpermatozoa quality parameters

Information

Type
Research Article
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 175 - 181
Copyright
Copyright © Cambridge University Press 2012 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Aisen, E.G., Medina, V.H. & Venturino, A. (2002). Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology 57, 1801–8.CrossRefGoogle ScholarPubMed
Amirat, L., Tainturier, D., Jeanneau, L., Thorin, C., Gerard, O., Courtens, J.L. & Anton, M. (2004). Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with optidyl, a commercial egg yolk extender. Theriogenology 61, 895–907.CrossRefGoogle ScholarPubMed
Amirat-Briand, L., Bencharif, D., Vera-Munoz, O., Bel Hadj Ali, H., Destrumelle, S., Desherces, S., Schmidt, E., Anton, M. & Tainturier, D. (2009). Effect of glutamine on post-thaw motility of bull spermatozoa after association with LDL (low-density lipoproteins) extender: Preliminary results. Theriogenology 71, 1209–14.CrossRefGoogle ScholarPubMed
Amirata, L., Tainturiera, D., Jeanneaua, L., Thorina, C., Gerard, O., Courtensc, J.L. & Antond, M. (2004). Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with optidyl, a commercial egg yolk extender. Theriogenology 61, 895–907.CrossRefGoogle Scholar
Babiak, I., Glogowski, J., Luczynski, M.J., Luczynski, M. & Demianowicz, W. (1999). The effect of egg yolk, low density lipoproteins, methylxanthines and fertilization diluent on cryopreservation efficiency of northern pike (Esox lucius) spermatozoa. Theriogenology 52, 473–9.CrossRefGoogle ScholarPubMed
Bair, C.W. & Marion, W.W. (1978). Yolk cholesterol in eggs from various avian species. Poult. Sci. 57, 1260–5.CrossRefGoogle ScholarPubMed
Bathgate, R., Maxwell, W.M. & Evans, G. (2006). Studies on the effect of supplementing boar semen cryopreservation media with different avian egg yolk types on in vitro post-thaw sperm quality. Reprod. Domest. Anim. 41, 68–73.CrossRefGoogle ScholarPubMed
Bergeron, A. & Manjunath, P. (2006). New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Mol. Reprod. Dev. 73, 1338–44.CrossRefGoogle ScholarPubMed
Demianowicz, W. & Strzezek, J. (1995). The effect of lipoprotein fraction from egg yolk on some of the biological properties of boar spermatozoa during storage of the semen in liquid state. Reprod. Domest. Anim. 31, 279–80.CrossRefGoogle Scholar
Evans, L.E. & Ozaki, H. (1973). Nuclear histones of unfertilized sea urchin eggs. Exp. Cell Res. 79, 228–31.CrossRefGoogle ScholarPubMed
Hu, J.H., Li, Q.W., Jiang, Z.L. & Li, W.Y. (2008). Effects of different extenders on DNA integrity of boar spermatozoa following freezing–thawing. Cryobiology 57, 257–62.CrossRefGoogle ScholarPubMed
Hu, J.H., Li, Q.W., Zan, L.S., Jiang, Z.L., An, J.H., Wang, L.Q. & Jia, Y.H. (2010). The cryoprotective effect of low-density lipoproteins in extenders on bull spermatozoa following freezing–thawing. Anim. Reprod. Sci. 117, 11–7.CrossRefGoogle ScholarPubMed
Hu, J.H., Jiang, Z.L., Lv, R.K., Li, Q.W., Zhang, S.S., Zan, L.S., Li, Y.K. & Li, X. (2011). The advantages of low-density lipoproteins in the cryopreservation of bull semen. Cryobiology 62, 83–7.CrossRefGoogle ScholarPubMed
Jiang, Z.L., Li, Q.W., Li, W.Y., Hu, J.H., Zhao, H.W. & Zhang, S.S. (2007). Effect of low density lipoprotein on DNA integrity of freezing–thawing boar sperm by neutral comet assay. Anim. Reprod. Sci. 99, 401–7.CrossRefGoogle ScholarPubMed
Kaeoket, K., Tantiparinyakul, K., Kladkaew, W., Chanapiawat, P. & Techakumphu, M. (2008). Effect of different antioxidants on quality of cryopreserved boar semen in different breeds. Thai J. Agr. Sci. 41, 1–9.Google Scholar
Kulaksz, R., Çebi, Ç. & Akçay, E. (2010). The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small Ruminant Res. 88, 12–15.CrossRefGoogle Scholar
Moussa, M., Marinet, V., Trimeche, A., Tainturier, D. & Anton, M. (2002). Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen–thawed bull semen. Theriogenology 57, 1695–706.CrossRefGoogle ScholarPubMed
Nauck, M. & Scharnagl, H. (1997). Mutations in the genes encoding the low-density lipoprotein receptor and apolipoprotein B in Southwest Germany. Atherosclerosis 134, 70–1.CrossRefGoogle Scholar
Pace, M.M. & Graham, E.F. (1974). Components in egg yolk which protect bovine spermatozoa during freezing. J. Anim. Sci. 39, 1144–9.CrossRefGoogle ScholarPubMed
Royle, N.J., Surai, P.F., Mccartney, R.J. & Speake, B.K. (1999). Parental investment and egg yolk lipid composition in gulls. Funct. Ecol. 13, 298–306.CrossRefGoogle Scholar
Sarnak, M.J., Tighiouart, H., Manjunath, G., MacLeod, B., Griffith, J., Salem, D. & Levey, A.S. (2002). Anemia as a risk factor for cardiovascular disease in the Atherosclerosis Risk in Communities (ARIC) study. J. Am. Coll. Cardiol. 40, 27–33.CrossRefGoogle ScholarPubMed
Su, L., Li, X.L., Quan, J.X., Yang, S.H., Li, Y.H., He, X.C. & Tang, X.H. (2008). A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm. Anim. Reprod. Sci. 104, 212–9.CrossRefGoogle Scholar
Thérien, R. & Manjunath, M. (1999). Bovine seminal plasma phospholipid-binding proteins stimulate phospholipid efflux from epididymal sperm. Biol. Reprod. 61, 590–8.CrossRefGoogle ScholarPubMed
Trimeche, A., Anton, M., Renard, B., Gandemer, G. & Tainturier, D. (1996). Quail egg yolk: A novel cryoprotectant for the freeze preservation of Poitou jackass sperm. Cryobiology 34, 385–93.CrossRefGoogle Scholar
Trimeche, A., Renard, P., Lannou, D.L., Barriere, P. & Tainturier, D. (1996). Improvement of motility of post-thaw Poitou jackass sperm using glutamine. Theriogenology 45, 1015–27.CrossRefGoogle ScholarPubMed
Tsutsui, T. (1988). Functional properties of heat-treated egg yolk low density lipoprotein. J. Food Sci. 53, 1103–6.CrossRefGoogle Scholar
Varela Junior, A.S., Corcini, C.D., Ulguim, R.R., Alvarenga, M.V.F., Bianchi, I., Corrêa, M.N., Lucia, Jr. T. & Deschamps, J.C. (2009). Effect of low density lipoprotein on the quality of cryopreserved dog semen. Anim. Reprod. Sci. 115, 323–7.CrossRefGoogle ScholarPubMed
Vera-Munoz, O., Amirat-Briand, L., Diaz, T., Vasquez, L., Schmidt, E., Desherces, S., Anton, M., Bencharif, D. & Tainturier, D. (2009). Effect of semen dilution to low-sperm number per dose on motility and functionality of cryopreserved bovine spermatozoa using low-density lipoproteins (LDL) extender: comparison to Triladyl (R) and Bioxcell (R). Theriogenology 71, 895–900.CrossRefGoogle Scholar
Zhou, J.B., Yue, K.Z., Sun, X.C., Liang, H., Chang, Z.L. & Tan, J.H. (2002). The study on technology of boar sperm cryopreservation. Chinese J. Vet. Sci. 22, 295–8.Google Scholar