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Detection of glycine receptor/Cl- channel beta subunit transcripts in mouse testis

Published online by Cambridge University Press:  19 June 2002

Yoko Sato ,
Richard P. Tucker  and
Stanley Meizel
Yoko Sato
Affiliation:
Department of Cell Biology and Human Anatomy, School of Medicine, University of California at Davis, CA 95616-8643, USA Present address: Department of Urology, St Marianna University School of Medicine, 2-16-1, Sugao, Miyamae-ku, Kawasaki, Kanagawa, 216-8511, Japan
Richard P. Tucker
Affiliation:
Department of Cell Biology and Human Anatomy, School of Medicine, University of California at Davis, CA 95616-8643, USA
Stanley Meizel
Affiliation:
Department of Cell Biology and Human Anatomy, School of Medicine, University of California at Davis, CA 95616-8643, USA
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Abstract

The sperm glycine receptor/Cl- channel (GlyR) is important to the initiation of the mammalian sperm acrosome reaction by the egg zona pellucida, but its presence in spermatogenic cells has not been demonstrated. Reverse transcriptase-polymerase chain reaction studies confirmed that GlyR beta subunit transcripts similar to those of the neuronal GlyR beta subunit are present in the testis of Swiss Webster mice. In situ hybridisation analysis demonstrated that GlyR beta subunit mRNAs were expressed within the germ cells of seminiferous tubules in those mice.

Keywords

Acrosome reactionIn situ hybridisationRT-PCRSperm

Information

Type
Research Article
Information
Zygote , Volume 10 , Issue 2 , May 2002 , pp. 105 - 108
Copyright
2002 Cambridge University Press

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