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Incidence of multiple herbicide resistance in annual bluegrass (Poa annua) across southeastern Australia

Published online by Cambridge University Press:  04 May 2020

Rajesh Barua Open the ORCID record for Rajesh Barua [Opens in a new window] ,
Peter Boutsalis ,
Jenna Malone Open the ORCID record for Jenna Malone [Opens in a new window] ,
Gurjeet Gill Open the ORCID record for Gurjeet Gill [Opens in a new window]  and
Christopher Preston Open the ORCID record for Christopher Preston [Opens in a new window]
Rajesh Barua*
Affiliation:
Postgraduate Student, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, Australia
Peter Boutsalis
Affiliation:
Postdoctoral Fellow, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, Australia
Jenna Malone
Affiliation:
Postdoctoral Fellow, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, Australia
Gurjeet Gill
Affiliation:
Associate Professor, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, Australia
Christopher Preston
Affiliation:
Professor, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, Australia
*
Author for correspondence: Rajesh Barua, School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, SA5064, Australia. Email: rajesh.barua@adelaide.edu.au
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Abstract

Annual bluegrass (Poa annua L.) is a problematic annual weed in established turf where the intensive use of herbicides has resulted in the evolution of herbicide resistance. In 2017, 31 populations of P. annua suspected to be resistant to herbicides commonly used to control this weed in turf were collected from golf courses across southeastern Australia to check the resistance status to different herbicide groups. All populations were found to be resistant to multiple turf herbicides. Dose–response experiments confirmed resistance to propyzamide, simazine, rimsulfuron, foramsulfuron, endothall, and pinoxaden. Levels of resistance to rimsulfuron (>56-fold), foramsulfuron (>19-fold), endothall (>7-fold), and pinoxaden (>4.3-fold) compared with the susceptible population were high, but levels of resistance to propyzamide (>2-fold) and simazine (>2-fold) were lower. Considerable variation in resistance to endothall and pinoxaden was observed among the populations of P. annua. Target-site resistance was confirmed for acetolactate synthase and acetyl-CoA carboxylase inhibitors, but not for photosystem II and microtubule assembly inhibitors. This study documented the extensive resistance to herbicides in P. annua from turf in Australia. Three of the populations investigated exhibited multiple resistance to herbicides from five mechanisms of action. The identification of multiple-resistant P. annua on several golf courses is a serious concern for turf managers.

Keywords

Endothallpinoxadenpropyzamidesimazineturfgrasswinter grass

Information

Type
Research Article
Information
Weed Science , Volume 68 , Issue 4 , July 2020 , pp. 340 - 347
Copyright
© Weed Science Society of America, 2020

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Footnotes

Associate Editor: Ramon G. Leon, North Carolina State University

References

Anonymous (2010) Rescue® product label. Crop Protection UK Limited. Capital Park, Cambridge, UK: Syngenta. 7 pGoogle Scholar
Ashworth, MB, Han, H, Knell, G, Powles, SB (2016 ) Identification of triazine-resistant Vulpia bromoides. Weed Technol 30:456–463CrossRefGoogle Scholar
Bajsa, J, Pan, Z, Dayan, FE, Owens, DK, Duke, SO (2012) Validation of serine/threonine protein phosphatase as the herbicide target site of endothall. Pestic Biochem Physiol 102:38–44CrossRefGoogle Scholar
Binkholder, KM, Fresenburg, BS, Teuton, TC, Xiong, X, Smeda, RJ (2011) Selection of glyphosate-resistant annual bluegrass (Poa annua) on a golf course. Weed Sci 59:286–289CrossRefGoogle Scholar
Boutsalis, P, Gill, GS, Preston, C (2012) Incidence of herbicide resistance in rigid ryegrass (Lolium rigidum) across southeastern Australia. Weed Technol 26:391–398CrossRefGoogle Scholar
Breeden, SM, Brosnan, JT, Mueller, TC, Breeden, GK, Horvath, BJ, Senseman, SA (2017) Confirmation and control of annual bluegrass (Poa annua) with resistance to prodiamine and glyphosate. Weed Technol 31:111–119CrossRefGoogle Scholar
Brosnan, J, Breeden, G (2013) Herbicide resistance in turfgrass: an emerging problem? Outlooks Pest Manag 24:164–168CrossRefGoogle Scholar
Brosnan, JT, Breeden, GK, Vargas, JJ, Grier, L (2015) A biotype of annual bluegrass (Poa annua) in Tennessee is resistant to inhibitors of ALS and photosystem II. Weed Sci 63:321–328CrossRefGoogle Scholar
Brosnan, JT, Vargas, JJ, Breeden, GK, Grier, L, Aponte, RA, Tresch, S, Laforest, M (2016) A new amino acid substitution (Ala-205-Phe) in acetolactate synthase (ALS) confers broad spectrum resistance to ALS-inhibiting herbicides. Planta 243:149–159CrossRefGoogle ScholarPubMed
Brunton, DJ, Boutsalis, P, Gill, G, Preston, C (2018) Resistance to multiple pre herbicides in a field-evolved rigid ryegrass (Lolium rigidum) population. Weed Sci 66:581–585CrossRefGoogle Scholar
Cross, RB, McCarty, LB, Tharayil, N, McElroy, JS, Chen, S, McCullough, PE, Powell, BA, Bridges, WC (2015) A Pro 106 to Ala substitution is associated with resistance to glyphosate in annual bluegrass (Poa annua). Weed Sci 63:613–622CrossRefGoogle Scholar
Cross, RB, McCarty, LB, Tharayil, N, Whitwell, T, Bridges, WC (2013) Detecting annual bluegrass (Poa annua) resistance to ALS-inhibiting herbicides using a rapid diagnostic assay. Weed Sci 61:384–389CrossRefGoogle Scholar
Darmency, H, Gasquez, J (1983) Interpreting the evolution of a triazine-resistant population of Poa annua L. New Phytol 95:299–304CrossRefGoogle Scholar
Délye, C (2013) Unravelling the genetic bases of non-target-site-based resistance (NTSR) to herbicides: a major challenge for weed science in the forthcoming decade. Pest Manag Sci 69:176–187CrossRefGoogle Scholar
Délye, C, Matéjicek, A, Gasquez, J (2002a) PCR-based detection of resistance to acetyl-CoA carboxylase-inhibiting herbicides in black-grass (Alopecurus myosuroides Huds) and ryegrass (Lolium rigidum Gaud). Pest Manag Sci 58:474–478CrossRefGoogle Scholar
Délye, C, Michel, S (2005) “Universal” primers for PCR-sequencing of grass chloroplastic acetyl-CoA carboxylase domains involved in resistance to herbicides. Weed Res 45:323–330CrossRefGoogle Scholar
Délye, C, Wang, T, Darmency, H (2002b) An isoleucine-leucine substitution in chloroplastic acetyl-CoA carboxylase from green foxtail (Setaria viridis (L.) Beauv.) is responsible for resistance to the cyclohexanedione herbicide sethoxydim. Planta 214:421–427CrossRefGoogle ScholarPubMed
Délye, C, Zhang, X-Q, Chalopin, C, Michel, S, Powles, SB (2003) An isoleucine residue within the carboxyl-transferase domain of multidomain acetyl-coenzyme A carboxylase is a major determinant of sensitivity to aryloxyphenoxypropionate but not to cyclohexanedione inhibitors. Plant Physiol 132:1716–1723CrossRefGoogle ScholarPubMed
Délye, C, Zhang, X-Q, Michel, S, Matéjicek, A, Powles, SB (2005) Molecular bases for sensitivity to acetyl-coenzyme A carboxylase inhibitors in black-grass. Plant Physiol 137:794–806CrossRefGoogle ScholarPubMed
Ellis, W (1973) The breeding system and variation in populations of Poa annua L. Evolution 27:656–662CrossRefGoogle ScholarPubMed
Fleet, B, Malone, J, Preston, C, Gill, G (2018) Target-site point mutation conferring resistance to trifluralin in rigid ryegrass (Lolium rigidum). Weed Sci 66:246–253CrossRefGoogle Scholar
Heap, I (2014) Herbicide resistant weeds. Pages 281–301in Pimentel, D, Peshin, R, eds. Integrated Pest Management. Dordrecht, Netherlands: SpringerCrossRefGoogle Scholar
Heap, I (2019) The International Survey of Herbicide Resistant Weeds. http://www.weedscience.org. Accessed: February 12, 2019Google Scholar
Heide, O (2001) Flowering responses of contrasting ecotypes of Poa annua and their putative ancestors Poa infirma andPoa supina. Ann Bot 87:795–804CrossRefGoogle Scholar
Herbert, D, Cole, DJ, Pallett, KE, Harwood, JL (1997) Graminicide-binding by acetyl-CoA carboxylase from Poa annua leaves. Phytochemistry 44:399–405CrossRefGoogle Scholar
Holm, LG, Holm, L, Holm, E, Pancho, JV, Herberger, JP (1997) World weeds: Natural Histories and Distribution. New York: Wiley, 609 pGoogle Scholar
Hutto, KC, Coats, GE, Taylor, JM (2004) Annual bluegrass (Poa annua) resistance to simazine in Mississippi. Weed Technol 18:846–849CrossRefGoogle Scholar
Isgrigg, J, Yelverton, FH, Brownie, C, Warren, LS (2002) Dinitroaniline resistant annual bluegrass in North Carolina. Weed Sci 50:86–90CrossRefGoogle Scholar
Kelly, ST, Coats, GE, Luthe, DS (1999) Mode of resistance of triazine-resistant annual bluegrass (Poa annua). Weed Technol 13:747–752CrossRefGoogle Scholar
Liu, W, Harrison, DK, Chalupska, D, Gornicki, P, O’Donnell, CC, Adkins, SW, Haselkorn, R, Williams, RR (2007) Single-site mutations in the carboxyltransferase domain of plastid acetyl-CoA carboxylase confer resistance to grass-specific herbicides. Proc Natl Acad Sci USA 104:3627–3632CrossRefGoogle ScholarPubMed
Lu, H, Yu, Q, Han, H, Owen, MJ, Powles, SB (2019) A novel psbA mutation (Phe274–Val) confers resistance to PSII herbicides in wild radish (Raphanus raphanistrum). Pest Manag Sci 75:144–151CrossRefGoogle Scholar
McCullough, PE, Yu, J, Czarnota, MA (2017) First report of pronamide-resistant annual bluegrass (Poa annua). Weed Sci 65:9–18CrossRefGoogle Scholar
McElroy, JS, Flessner, ML, Wang, Z, Dane, F, Walker, RH, Wehtje, GR (2013) A Trp 574 to Leu amino acid substitution in the ALS gene of annual bluegrass (Poa annua) is associated with resistance to ALS-inhibiting herbicides. Weed Sci 61:21–25CrossRefGoogle Scholar
McElroy, JS, Walker, RH, Wehtje, GR, Van Santen, E (2004) Annual bluegrass (Poa annua) populations exhibit variation in germination response to temperature, photoperiod, and fenarimol. Weed Sci 52:47–52CrossRefGoogle Scholar
Mengistu, LW, Mueller-Warrant, GW, Liston, A, Barker, RE (2000) psbA mutation (valine219 to isoleucine) in Poa annua resistant to metribuzin and diuron. Pest Manag Sci 56:209–2173.0.CO;2-8>CrossRefGoogle Scholar
Perry, D, McElroy, J, Dane, F, Van Santen, E, Walker, R (2012) Triazine-resistant annual bluegrass (Poa annua) populations with Ser 264 mutation are resistant to amicarbazone. Weed Sci 60:355–359CrossRefGoogle Scholar
Powles, SB, Lorraine-Colwill, DF, Dellow, JJ, Preston, C (1998) Evolved resistance to glyphosate in rigid ryegrass (Lolium rigidum) in Australia. Weed Sci 46:604–607CrossRefGoogle Scholar
Sakuma, M (1998) Probit analysis of preference data. Appl Entomol Zool 33:339–347CrossRefGoogle Scholar
Schibler, MJ, Huang, B (1991) The colR4 and colR15 beta-tubulin mutations in Chlamydomonas reinhardtii confer altered sensitivities to microtubule inhibitors and herbicides by enhancing microtubule stability. J Cell Biol 113:605–614CrossRefGoogle ScholarPubMed
Svyantek, A, Aldahir, P, Chen, S, Flessner, M, McCullough, P, Sidhu, S, McElroy, J (2016) Target and non-target resistance mechanisms induce annual bluegrass (Poa annua) resistance to atrazine, amicarbazone, and diuron. Weed Technol 30:773–782CrossRefGoogle Scholar
Takahashi, A, Yamada, S, Tanaka, K (2002) Mechanism of herbicidal activity of a new cyclohexane-1,3-dion, tepraloxydim to Poa annua L. Weed Biol Manag 2:84–91CrossRefGoogle Scholar
Thiel, H, Varrelmann, M (2014) Identification of a new PSII target site psbA mutation leading to D1 amino acid leu218val exchange in the Chenopodium album D1 protein and comparison to cross-resistance profiles of known modifications at positions 251 and 264. Pest Manag Sci 70:278–285CrossRefGoogle Scholar
Toler, JE, Willis, TG, Estes, AG, McCarty, LB (2007) Postemergent annual bluegrass control in dormant nonoverseeded bermudagrass turf. HortScience 42:670–672CrossRefGoogle Scholar
Tresch, S, Schmotz, J, Grossmann, K (2011) Probing mode of action in plant cell cycle by the herbicide endothall, a protein phosphatase inhibitor. Pestic Biochem Physiol 99:86–95CrossRefGoogle Scholar
Wagner, W, Herbst, D, Sohmer, S (1990) Manual of the flowering plants of Hawai‘i. 2 vols. Honolulu, HI: University of Hawai‘i Press and Bishop Museum Press. 1853 pGoogle Scholar
[WSSA] Weed Science Society of America (2020) WSSA Herbicide Mode of Action Table 2020. http://weedscience.org/summary/soadescription.aspx. Accessed: 27 Apr 2020Google Scholar
Yu, Q, Han, H, Powles, SB (2008) Mutations of the ALS gene endowing resistance to ALS-inhibiting herbicides in Lolium rigidum populations. Pest Manag Sci 64:1229–1236CrossRefGoogle ScholarPubMed
Zhang, X-Q, Powles, SB (2006a) The molecular bases for resistance to acetyl co-enzyme A carboxylase (ACCase) inhibiting herbicides in two target-based resistant biotypes of annual ryegrass (Lolium rigidum). Planta 223:550–557CrossRefGoogle Scholar
Zhang, X-Q, Powles, SB (2006b) Six amino acid substitutions in the carboxyl-transferase domain of the plastidic acetyl-CoA carboxylase gene are linked with resistance to herbicides in a Lolium rigidum population. New Phytol 172:636–645CrossRefGoogle Scholar