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Distribution of the ΔG210 Protoporphyrinogen Oxidase Mutation inIllinois Waterhemp (Amaranthus tuberculatus) and anImproved Molecular Method for Detection

Published online by Cambridge University Press:  20 January 2017

R. Joseph Wuerffel ,
Julie M. Young ,
Ryan M. Lee ,
Patrick J. Tranel ,
David A. Lightfoot  and
Bryan G. Young
R. Joseph Wuerffel*
Affiliation:
Department of Plant, Soil, and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901
Julie M. Young
Affiliation:
Botany and Plant Pathology Department, Purdue University, West Lafayette, IN 47907
Ryan M. Lee
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Patrick J. Tranel
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
David A. Lightfoot
Affiliation:
Department of Plant, Soil, and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901
Bryan G. Young
Affiliation:
Botany and Plant Pathology Department, Purdue University, West Lafayette, IN 47907
*
Corresponding author's E-mail: rwuerff@gmail.com
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Abstract

Molecular assays are often implemented by weed scientists for detection ofherbicide-resistant individuals; however, the utility of these assays can belimited if multiple mechanisms of evolved resistance exist. Waterhempresistant to protoporphyrinogen oxidase (PPO)– inhibiting herbicides isconferred by a target-site mutation in PPX2L (a gene codingfor PPO), resulting in the loss of a glycine at position 210 (ΔG210). ThisΔG210 mutation of PPX2L is the only known mechanismresponsible for PPO-inhibitor resistance (PPO-R) in waterhemp from fivestates (Illinois, Indiana, Iowa, Kansas, and Missouri); however, a limitednumber of populations have been tested, especially in Illinois. To verifythe ubiquity of the ΔG210 in PPO-R waterhemp populations in Illinois, apreviously published allele-specific PCR (asPCR) was used for the detectionof the ΔG210 mutation to associate this mutation with phenotypic resistancein 94 Illinois waterhemp populations. The ΔG210 mutation was detected in allpopulations displaying phenotypic resistance to lactofen (220 g ai ha−1), indicating the deletion is likely the only mechanism ofresistance. With evidence that the ΔG210 mutation dominates PPO-R waterhempbiotypes, molecular detection techniques have considerable utility.Unfortunately, the previously published asPCR is time consuming, verysensitive to PCR conditions, and requires additional steps to eliminate thepossibility of false negatives. To overcome these limitations, a streamlinedmolecular method using the TaqMan® technique was developed, utilizingallele-specific, fluorescent probes for high-throughput, robustdiscrimination of each allele (resistant and susceptible) at the 210th aminoacid position of PPX2L.

Keywords

Lactofenwaterhemp, Amaranthus tuberculatus (Moq.) Sauer (syn. rudis) AMATA.PPO-inhibiting herbicidesherbicide resistancegenotypingpolymorphismqPCR

Information

Type
Weed Biology and Ecology
Information
Weed Science , Volume 63 , Issue 4 , December 2015 , pp. 839 - 845
Copyright
Copyright © Weed Science Society of America 

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Footnotes

Associate Editor for this paper: Franck E. Dayan, USDAARS-NPURU.

References

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