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Colocalization of substance P and GABA in retinal ganglion cells: A computer-assisted visualization

Published online by Cambridge University Press:  02 June 2009

Daniel M. Caruso ,
Michael T. Owczarzak  and
Roberta G. Pourcho
Daniel M. Caruso
Affiliation:
Department of Anatomy and Cell Biology, Wayne State University, Detroit
Michael T. Owczarzak
Affiliation:
Department of Anatomy and Cell Biology, Wayne State University, Detroit
Roberta G. Pourcho
Affiliation:
Department of Anatomy and Cell Biology, Wayne State University, Detroit
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Abstract

Ganglion cells in the albino rat retina were retrogradely labeled with the fluorescent dye, diamidino-yellow, from the superior colliculus. Preembedding and postembedding immunocytochemical techniques were employed in conjunction with computer-assisted image processing to visualize SP- and GABA-immunoreactivity. Examination of flatmount and sectioned retinas revealed that approximately 3% of the ganglion cells projecting to the contralateral superior colliculus exhibit SP-immunoreactivity. Moreover, these cells were found to comprise a subpopulation of the GABA-immunoreactive cells projecting to the rat tectum.

Keywords

Substance PGABAGanglion cellsRetinaTriple localization

Information

Type
Research Article
Information
Visual Neuroscience , Volume 5 , Issue 4 , October 1990 , pp. 389 - 394
Copyright
Copyright © Cambridge University Press 1990

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