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Time–resolved capacitance measurements: monitoring exocytosis in single cells

Published online by Cambridge University Press:  17 March 2009

Manfred Lindau
Manfred Lindau
Affiliation:
Biophysics Group, Freie Universität Berlin, Arnimallee 14, D–1000 Berlin 33, Germany
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Extract

Many cells release preformed material contained in secretory granules by exocytosis. Exocytosis is a specialized means of secretion in which the granules fuse with the plasma membrane and thereby discharge their contents through the fusion pores. This mechanism mediates, for example, the formation of the fertilization envelope in eggs, the release of neurotransmitters and neuropeptides by neurons, the release of a variety of enzymes and mediators by mast cells and granulocytes or the secretion of hormones by endocrine cells. Classical methods for investigating exocytosis usually measure release of secreted material.

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Type
Research Article
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Quarterly Reviews of Biophysics , Volume 24 , Issue 1 , February 1991 , pp. 75 - 101
Copyright
Copyright © Cambridge University Press 1991

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