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Fast protein folding kinetics

Published online by Cambridge University Press:  18 March 2014

Hannah Gelman  and
Martin Gruebele
Hannah Gelman
Affiliation:
Departments of Physics, Chemistry, and Center for Biophysics and Computational Biology, University of Illinois, Urbana, IL 61801, USA
Martin Gruebele*
Affiliation:
Departments of Physics, Chemistry, and Center for Biophysics and Computational Biology, University of Illinois, Urbana, IL 61801, USA
*
*Author for Correspondence: Martin Gruebele, Departments of Physics, Chemistry, and Center for Biophysics and Computational Biology, University of Illinois, 600 S. Mathews Avenue, RAL 29A, Urbana, IL 61801, USA. Tel: (217) 333-6136; Fax: (217) 244-3186; Email: mgruebel@illinois.edu
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Abstract

Fast-folding proteins have been a major focus of computational and experimental study because they are accessible to both techniques: they are small and fast enough to be reasonably simulated with current computational power, but have dynamics slow enough to be observed with specially developed experimental techniques. This coupled study of fast-folding proteins has provided insight into the mechanisms, which allow some proteins to find their native conformation well <1 ms and has uncovered examples of theoretically predicted phenomena such as downhill folding. The study of fast folders also informs our understanding of even ‘slow’ folding processes: fast folders are small; relatively simple protein domains and the principles that govern their folding also govern the folding of more complex systems. This review summarizes the major theoretical and experimental techniques used to study fast-folding proteins and provides an overview of the major findings of fast-folding research. Finally, we examine the themes that have emerged from studying fast folders and briefly summarize their application to protein folding in general, as well as some work that is left to do.

Type
Review Article
Information
Quarterly Reviews of Biophysics , Volume 47 , Issue 2 , May 2014 , pp. 95 - 142
Copyright
Copyright © Cambridge University Press 2014 

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