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Published online by Cambridge University Press: 22 November 2017
Salmonellosis is an infectious disease caused by Salmonella spp. that continues to plague human populations in both developed and third-world countries (Bohórquez, 2007). Conventional culture-based methods of detection that rely on enrichment of the sample and plating onto selective agar media may not be as sensitive as immunologic-or genetic-based rapid methods (Feng, 2001). PCR-based methods offer the advantages of high specificity and sensitivity. Some efforts have been made to reduce the time required and to increase the sensitivity and the accuracy of the methods to quantification of Salmonella in poultry samples (Mandrell and Wachtel 1999). This study was conducted in order to determine the relative population of Salmonella spp. in the duodenum, jejunum, ileum and caecum of chickens at different ages using densitometry.