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Microsatellite fingerprinting in the International Cocoa Genebank, Trinidad: accession and plot homogeneity information for germplasm management

Published online by Cambridge University Press:  20 April 2011

Lambert A. Motilal ,
Dapeng Zhang ,
Pathmanathan Umaharan ,
Sue Mischke ,
Stephen Pinney  and
Lyndel W. Meinhardt
Lambert A. Motilal*
Affiliation:
Cocoa Research Unit, The University of the West Indies, St. Augustine, Trinidad, Rep. Trinidad and Tobago, West Indies
Dapeng Zhang
Affiliation:
USDA/ARS, Beltsville Agricultural Research Center, PSI, SPCL, 10300 Baltimore Avenue, Bldg. 001, Rm. 223, BARC-W, Beltsville, MD 20705, USA
Pathmanathan Umaharan
Affiliation:
Cocoa Research Unit, The University of the West Indies, St. Augustine, Trinidad, Rep. Trinidad and Tobago, West Indies
Sue Mischke
Affiliation:
USDA/ARS, Beltsville Agricultural Research Center, PSI, SPCL, 10300 Baltimore Avenue, Bldg. 001, Rm. 223, BARC-W, Beltsville, MD 20705, USA
Stephen Pinney
Affiliation:
USDA/ARS, Beltsville Agricultural Research Center, PSI, SPCL, 10300 Baltimore Avenue, Bldg. 001, Rm. 223, BARC-W, Beltsville, MD 20705, USA
Lyndel W. Meinhardt
Affiliation:
USDA/ARS, Beltsville Agricultural Research Center, PSI, SPCL, 10300 Baltimore Avenue, Bldg. 001, Rm. 223, BARC-W, Beltsville, MD 20705, USA
*
*Corresponding author. E-mail: lamotilal@yahoo.com
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Abstract

The International Cocoa Genebank, Trinidad, is the largest field genebank collection of cacao (Theobroma cacao L.) in the public domain and the correct identity of each tree is crucial for germplasm movement, evaluation and phenotypic characterization. Nine microsatellite loci were used to assess the identity of 1477 trees from 486 cacao accessions representing approximately 16.9% of the trees and 29.2% of the accessions within the genebank. Heterogeneous plots (plots containing more than one genotype group) averaged 25.1% in The International Cocoa Genebank, Trinidad, with maximal admixture (32.6%) being recorded in Field 5B. The error rate did not differ significantly among different fields. Mislabeling error could be affected by accession grouping with an average error rate of 27.4% for accession groups in the genebank. Synonymous accessions were estimated to account for 14.4% of the field genebank. The results of the present study provide essential information for the management and utilization of the germplasm collection. Single-tree genotyping of every tree in this collection is strongly recommended.

Keywords

field genebankmislabellingsimple sequence repeatTheobroma cacaotree identityverification

Information

Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 3 , August 2011 , pp. 430 - 438
Copyright
Copyright © NIAB 2011

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