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Identification of Hydrangeaceae accessions of wild origin from Jeju, Korea, using molecular markers

Published online by Cambridge University Press:  24 September 2010

Young Hee Joung ,
Jeung Keun Suh ,
Nam Sook Lee ,
Sang Mi Eum ,
Ik-Young Choi  and
Mark S. Roh
Young Hee Joung
Affiliation:
Chonnam National University, School of Biological Science and Technology, Gwangju, Jeonnam, Korea
Jeung Keun Suh
Affiliation:
Dankook University, School of Bio-Resources Science, Laboratory of Floriculture and Plant Physiology, Cheonan, Chungnam, Korea
Nam Sook Lee
Affiliation:
Ewha Womans University, Department of Life Science, Seoul 120-750, Korea
Sang Mi Eum
Affiliation:
Ewha Womans University, Department of Life Science, Seoul 120-750, Korea
Ik-Young Choi
Affiliation:
National Instrumentation Center for Environmental Management, Seoul National University, Seoul, Korea
Mark S. Roh*
Affiliation:
US Department of Agriculture, Agricultural Research Service, US National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA
*
*Corresponding author. E-mail: mark.roh@ars.usda.gov
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Abstract

Hydrangea and Schizophragma are popular horticultural and ornamental plants. This study was designed to identify unknown accessions of Hydrangeaceae that have leaf morphological characters different from Hydrangea anomala subsp. petiolaris and Schizophragma hydrangeoides in Jeju, Korea. A total of 29 accessions of Hydrangea and Schizophragma from the wild were collected and analyzed using randomly amplified polymorphic DNA (RAPD) and single nucleotide polymorphisms (SNPs) from the ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene. Morphological characteristics of leaves and shoot apices of several accessions were also examined. Based on the RAPD markers, SNPs of the (rbcL) gene and morphological characteristics of apical buds, the unknown accessions were identified as H. anomala var. petiolaris.

Keywords

germplasmHydrangea anomala subsp. petiolarismorphologynucleotide polymorphisms (SNPs)Schizophragma hydrangeoides
Type
Research Article
Information
Plant Genetic Resources , Volume 8 , Issue 3 , December 2010 , pp. 235 - 241
Copyright
Copyright © NIAB 2010

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