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Evaluation of French bean germplasm from Garhwal Himalayas for resistance to angular leaf spot

Published online by Cambridge University Press:  08 March 2023

Navneeti Chamoli ,
Deepti Prabha Open the ORCID record for Deepti Prabha [Opens in a new window] ,
Yogesh Kumar Negi  and
Jai Singh Chauhan
Navneeti Chamoli
Affiliation:
Department of Seed Science & Technology, School of Agriculture and Allied Sciences, HNB Garhwal University, Srinagar, Uttarakhand, India
Deepti Prabha*
Affiliation:
Department of Seed Science & Technology, School of Agriculture and Allied Sciences, HNB Garhwal University, Srinagar, Uttarakhand, India
Yogesh Kumar Negi
Affiliation:
Department of Basic Sciences, College of Forestry, (VCSG UUHF), Ranichauri, Tehri Garhwal, Uttarakhand, India
Jai Singh Chauhan
Affiliation:
Department of Seed Science & Technology, School of Agriculture and Allied Sciences, HNB Garhwal University, Srinagar, Uttarakhand, India
*
Author for correspondence: Deepti Prabha, E-mail: deepti_prabha@rediffmail.com
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Abstract

Angular leaf spot (ALS) caused by Pseudocercospora griseola is a major disease of french bean (Phaseolus vulgaris L.) worldwide. A good diversity of French bean is present in the Garhwal Himalayas of Uttarakhand, India, which is unexplored. The purpose of this study was to identify ALS-resistant accessions among local landraces of French bean in this region. One hundred seventy-six local accessions were collected from different villages of Garhwal, Uttarakhand. All the accessions were screened by four SCAR primers SN02 (Phg-2), SAA19, SM02, SBA16 (Phg-3), one STS primer TGA1.1 (Phg-1) and one SSR primer Pv-at006 (Phg-5). All the accessions were also screened for ALS resistance under field condition in the years 2019 and 2020. The disease-resistant score was recorded on 1–9 scale. After field screening, 48 accessions (19 resistant, 24 moderately resistant and five susceptible) were selected for in-vitro screening under screen house condition. These 46 accessions were artificially inoculated by two different isolates of P. griseola P5 and P9, which are the most virulent pathotype characterized by microbiology lab, College of Forestry, Tehri, Uttarakhand. After in-vitro screening, seven accessions (GFB-25, GFB-26, GFB-30, GFB-32, GFB-93, GFB-97 and GFB-136) were found resistant to both the isolates P5 and P9. The P. griseola-resistant accessions may further be used in future breeding programmes to develop new and more resistant varieties of French bean against ALS.

Keywords

ALSdisease reactionFrench beanmolecular markerPhg genes
Type
Research Article
Information
Plant Genetic Resources , Volume 20 , Issue 4 , August 2022 , pp. 255 - 262
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of NIAB

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