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QTL mapping for seedling morphology under drought stress in wheat cross synthetic (W7984)/Opata

Published online by Cambridge University Press:  13 March 2018

Maria Khalid ,
Alvina Gul ,
Rabia Amir ,
Mohsin Ali ,
Fakiha Afzal ,
Umar Masood Quraishi ,
Zubair Ahmed  and
Awais Rasheed
Maria Khalid
Affiliation:
Atta-ur-Rehman School of Applied Biosciences (ASAB), National University of Science and Technology (NUST), Islamabad, Pakistan
Alvina Gul
Affiliation:
Atta-ur-Rehman School of Applied Biosciences (ASAB), National University of Science and Technology (NUST), Islamabad, Pakistan School of Integrative Plant Science, Cornell University, Ithaca, NY 14850, USA
Rabia Amir
Affiliation:
Atta-ur-Rehman School of Applied Biosciences (ASAB), National University of Science and Technology (NUST), Islamabad, Pakistan
Mohsin Ali
Affiliation:
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing 100081, China
Fakiha Afzal
Affiliation:
Atta-ur-Rehman School of Applied Biosciences (ASAB), National University of Science and Technology (NUST), Islamabad, Pakistan
Umar Masood Quraishi
Affiliation:
Department of Plant Sciences, Quaid-i-Azam UniversityIslamabad 66000, Pakistan
Zubair Ahmed
Affiliation:
Crop Science Institute, National Agricultural Research Centre, Islamabad, Pakistan
Awais Rasheed*
Affiliation:
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing 100081, China International Maize and Wheat Improvement Center (CIMMYT), c/o CAAS, 12 Zhongguancun South Street, Beijing 100081, China
*
*Corresponding author. E-mail: awais_rasheed@yahoo.com; a.rasheed@cgiar.org
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Abstract

Drought stress ‘particularly at seedling stage’ causes morpho-physiological differences in wheat which are crucial for its survival and adaptability. In the present study, 209 recombinant inbred lines (RILs) from synthetic wheat (W7984)× ‘Opata’ (also known as SynOpRIL) population were investigated under well-watered and water-limited conditions to identify quantitative trait loci (QTL) for morphological traits at seedling stage. Analysis of variance revealed significant differences (P < 0.01) among RILs, and water treatments for all traits with moderate to high broad sense heritability. Pearson's coefficient of correlation revealed positive correlation among all traits except dry root weight that showed poor correlation with fresh shoot weight (FSW) under water-limited conditions. A high-density linkage map was constructed with 2639 genotyping-by-sequencing markers and covering 5047 cM with an average marker density of 2 markers/cM. Composite interval mapping identified 16 QTL distributed over nine chromosomes, of which six were identified under well-watered and 10 in water-limited conditions. These QTL explained from 4 to 59% of the phenotypic variance. Six QTL were identified on chromosome 7B; three for shoot length under water-limited conditions (QSL.nust-7B) at 64, 104 and 221 cM, two for fresh root weight (QFRW.nust-7B) at 124 and 128 cM, and one for root length (QRL.nust-7B) at 122 cM positions. QFSW.nust-7B appeared to be the most significant QTL explaining 59% of the phenotypic variance and also associated with FSW at well-watered conditions. These QTL could serve as target regions for candidate gene discovery and marker-assisted selection in wheat breeding.

Keywords

analysis of variance (ANOVA)broad sense heritabilitydroughtInternational Triticeae Mapping Initiative (ITMI)Pearson's correlationQTL mappingwheat
Type
Research Article
Information
Plant Genetic Resources , Volume 16 , Issue 4 , August 2018 , pp. 359 - 366
Copyright
Copyright © NIAB 2018 

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