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A baseline study of vicine–convicine levels in faba bean (Vicia faba L.) germplasm

Published online by Cambridge University Press:  30 April 2013

Khalil Khamassi ,
Fayçal Ben Jeddi ,
Doug Hobbs ,
Jose Irigoyen ,
Fred Stoddard ,
Donal M. O'Sullivan  and
Huw Jones
Khalil Khamassi
Affiliation:
National Institute of Agricultural Botany, Huntingdon Road, CambridgeCB3 0LE, UK Institut National Agronomique de Tunisie (INAT), Unité de Recherche Cultures Maraîchères et Florale, 43 Avenue Charles Nicolle 1082 cité Mahrajène, Tunis, Tunisia
Fayçal Ben Jeddi
Affiliation:
Institut National Agronomique de Tunisie (INAT), Unité de Recherche Cultures Maraîchères et Florale, 43 Avenue Charles Nicolle 1082 cité Mahrajène, Tunis, Tunisia
Doug Hobbs
Affiliation:
National Institute of Agricultural Botany, Huntingdon Road, CambridgeCB3 0LE, UK
Jose Irigoyen
Affiliation:
National Institute of Agricultural Botany, Huntingdon Road, CambridgeCB3 0LE, UK
Fred Stoddard
Affiliation:
University of Helsinki, Helsinki, Finland
Donal M. O'Sullivan
Affiliation:
National Institute of Agricultural Botany, Huntingdon Road, CambridgeCB3 0LE, UK Department of Agriculture, Policy and Development, University of Reading, Reading, UK
Huw Jones*
Affiliation:
National Institute of Agricultural Botany, Huntingdon Road, CambridgeCB3 0LE, UK
*
*Corresponding author. E-mail: huw.jones@niab.com
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Abstract

Vicine and convicine are anti-nutritional compounds that accumulate in the cotyledons of faba beans. When humans consume beans with high levels of these compounds, it can cause a condition called favism in individuals harbouring a deficiency in the activity of their glucose-6-phosphate dehydrogenase. When faba beans are used in animal feeds, there can be effects on performance. These concerns have resulted in increasing interest within plant breeding in developing low vicine and convicine faba bean germplasm. In order to facilitate this objective, we developed a rapid and robust screening method for vicine and convicine, capable of distinguishing between faba beans that are either high (wild type) or low in vicine and convicine. In the absence of reliable commercial reference materials, we report an adaptation of a previously published method where a biochemical assay and spectral data were used to confirm the identity of our analytes, vicine and convicine. This method could be readily adopted in other facilities and open the way to the efficient exploitation of diverse germplasm in regions where faba beans play a significant role in human nutrition. We screened a collection of germplasm of interest to a collaborative plant breeding programme developing between the National Institute for Agricultural Botany in the UK and L'Institut Nationale d'Agronomie de Tunisie in Tunisia. We report the results obtained and discuss the prospects for developing molecular markers for the low vicine and convicine trait.

Keywords

faba beanHPLCViciavicine–convicine
Type
Research Article
Information
Plant Genetic Resources , Volume 11 , Issue 3 , December 2013 , pp. 250 - 257
Copyright
Copyright © NIAB 2013 

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References

Arbid, MSS and Marquardt, RR (1985) Hydrolysis of the toxic constituents (vicine and convicine) in Faba bean (Vicia faba L.) food preparations following treatment with ß-glucosidase. Journal of the Science of Food and Agriculture 36: 839–846.Google Scholar
Arese, P, Bosia, A, Naitana, A, Gaetani, S, D'Aquino, M and Gaetani, GF (1981) Effect of divicine and isouramil on red cell metabolism in normal and G6PD-deficient (Mediterranean variant) subjects. Possible role in the genesis of favism. Progress in Clinical Biological Research 55: 725–746.Google Scholar
Brown, AT and Morrall, P (1996) Determination of repeatability and reproducibility of a new rapid enzyme method for the determination of glycoside nitrile in malted barley. Journal of the Institute of Brewing 102: 245–247.Google Scholar
Burbano, C, Cuadrado, C, Muzquiz, M and Cubero, JI (1995) Variation of favism-inducing factors (vicine, convicine and l-DOPA) during pod development in Vicia faba L. Plant Foods for Human Nutrition 47: 265–275.Google Scholar
Chevion, M and Navok, T (1983) A novel method for quantitation of favism-inducing agents in legumes. Analytical Biochemistry 128: 152–158.CrossRefGoogle ScholarPubMed
Cottage, A, Gostkiewicz, K, Thomas, J, Borrows, R, Torres, AM and O'Sullivan, D (2012) Heterozygosity and diversity analysis using mapped single nucelotide polymorphisms in a faba bean inbreeding programme. Molecular Breeding 30: 1799–1809.CrossRefGoogle Scholar
Crépon, K, Marget, P, Peyronnet, C, Carrouée, B, Arese, P and Duc, G (2010) Nutritional value of faba bean (Vicia faba L.) seeds for feed and food. Field Crops Research 115: 329–339.CrossRefGoogle Scholar
Duc, G, Sixdenier, G, Lila, M and Furstoss, V (1989) Search of genetic variability for vicine and convicine content in Vicia faba L. A first report of a gene which codes for nearly zero-vicine and zero-convicine contents. In: Huisman, J, van der Poel, TFB and Liener, IE (eds) Recent Advances of Research in Antinutritional Factors in Legumes Seeds. Pudoc: Wageningen, pp. 305–313.Google Scholar
Duc, G, Bao, S, Baum, M, Redden, B, Sadiki, M, Suso, MJ, Vishniakova, M and Zong, X (2010) Diversity maintenance and use of Vicia faba L. genetic resources. Field Crops Research 115: 270–278.CrossRefGoogle Scholar
Greene, LS (1993) G6PD deficiency as protection against falciparum malaria: an epidemiologic critique of population and experimental studies. American Journal of Physical Anthropology 36: 153–178.Google Scholar
Gutierrez, N, Avila, CM, Duc, G, Marget, P, Suso, MJ, Moreno, MT and Torres, AM (2006) CAPs markers to assist selection for low vicine and convicine contents in faba bean (Vicia faba L.). Theoretical and Applied Genetics 114: 59–66.CrossRefGoogle ScholarPubMed
Jamalian, J, Aylward, F and Hudson, BJF (1977) Favism-inducing toxins in broad beans (Vicia faba): estimation of the vicine contents of broad bean and other legume samples. Plant Foods for Human Nutrition (Formerly Qualitas Plantarum) 27: 207–211.Google Scholar
Kaufmann, B and Christen, P (2002) Recent extraction techniques for natural products: microwave-assisted extraction and pressurised solvent extraction. Phytochemical Analysis 13: 105–113.CrossRefGoogle ScholarPubMed
Kim, SI, Hoehm, E, Eskin, NAM and Ismail, F (1982) A simple and rapid colorimetric method for determination of vicine and convicine. Journal of Agricultural and Food Chemistry 30: 144–147.CrossRefGoogle Scholar
Lattanzio, V, Bianco, VV and Lafiandra, D (1982) High performance reversed-phase liquid chromatography (HPLC) of favism inducing factors in Vicia faba L. Experimentia 30: 789–790.Google Scholar
Marquardt, RR, Campbell, LD and Ward, T (1976) Studies with chicks on the growth depressing factor(s) in faba beans (Vicia faba L. var. minor). The Journal of Nutrition 106: 275–284.CrossRefGoogle ScholarPubMed
Marquardt, RR, Muduuli, DS and Frohlich, AA (1983) Purification and some properties of vicine and convicine isolated from faba bean (Vicia faba L.) protein concentrate. Journal of Agricultural and Food Chemistry 31: 839–844.Google Scholar
Muduuli, DS, Marquardt, RR and Guenter, W (1982) Effect of dietary vicine and vitamin E supplementation on the productive performance of growing and laying chickens. British Journal of Nutrition 47: 53–60.CrossRefGoogle ScholarPubMed
Windholz, M, Budavari, S and Stroumtsos, LY and Fertig, MN (eds) (1976) The Merck Index: An Encyclopaedia of Chemicals and Drugs. 9th ed.Rahway, NJ: Merck & Co., Inc., p. 266.Google Scholar
Olsen, HS and Andersen, JH (1978) The estimation of vicine and convicine in faba beans (Vicia faba L.) and isolated faba bean proteins. Journal of the Science of Food and Agriculture 29: 323–331.Google Scholar
Wang, P-X and Ueberschär, KH (1990) The estimation of vicine, convicine and condensed tannins in 22 varieties of faba beans (Vicia faba L.). Animal Feed Science and Technology 31: 157–165.CrossRefGoogle Scholar
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