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Trypanocidal activity of free and nanoencapsulated curcumin on Trypanosoma evansi

Published online by Cambridge University Press:  19 August 2014

L. T. GRESSLER ,
C. B. OLIVEIRA ,
K. CORADINI ,
L. DALLA ROSA ,
T. H. GRANDO ,
M. D. BALDISSERA ,
C. E. ZIMMERMANN ,
A. S. DA SILVA ,
T. C. ALMEIDA  and
C. L. HERMES
...Show all authors
L. T. GRESSLER*
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil
C. B. OLIVEIRA
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil
K. CORADINI
Affiliation:
Faculdade de Farmácia, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil
L. DALLA ROSA
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil
T. H. GRANDO
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil
M. D. BALDISSERA
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil
C. E. ZIMMERMANN
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil
A. S. DA SILVA
Affiliation:
Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, Brazil
T. C. ALMEIDA
Affiliation:
Department of Clinical and Toxicological Analysis, UFSM, Santa Maria, Brazil
C. L. HERMES
Affiliation:
Department of Clinical and Toxicological Analysis, UFSM, Santa Maria, Brazil
P. WOLKMER
Affiliation:
Universidade de Cruz Alta (UNICRUZ), Cruz Alta, Brazil
C. B. SILVA
Affiliation:
Department of Small Animals, UFSM, Santa Maria, Brazil
K. L. S. MOREIRA
Affiliation:
Department of Morphology, UFSM, Santa Maria, Brazil
R. C. R. BECK
Affiliation:
Faculdade de Farmácia, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil
R. N. MORESCO
Affiliation:
Department of Clinical and Toxicological Analysis, UFSM, Santa Maria, Brazil
M. L. DA VEIGA
Affiliation:
Universidade de Cruz Alta (UNICRUZ), Cruz Alta, Brazil
L. M. STEFANI
Affiliation:
Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, Brazil
S. G. MONTEIRO
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil
*
* Corresponding author: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil. E-mail: lucastrevi@hotmail.com
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Summary

This study aimed to evaluate in vitro and in vivo trypanocidal activity of free and nanoencapsulated curcumin against Trypanosoma evansi. In vitro efficacy of free curcumin (CURC) and curcumin-loaded in lipid-core nanocapsules (C-LNCs) was evaluated to verify their lethal effect on T. evansi. To perform the in vivo tests, T. evansi-infected animals were treated with CURC (10 and 100 mg kg−1, intraperitoneally [i.p.]) and C-LNCs (10 mg kg−1, i.p.) during 6 days, with the results showing that these treatments significantly attenuated the parasitaemia. Infected untreated rats showed protein peroxidation and an increase of nitrites/nitrates, whereas animals treated with curcumin showed a reduction on these variables. As a result, the activity of antioxidant enzymes (superoxide dismutase and catalase) differs between groups (P<0·05). Infected animals and treated with CURC exhibited a reduction in the levels of alanine aminotransferase and creatinine, when compared with the positive control group. The use of curcumin in vitro resulted in a better parasitaemia control, an antioxidant activity and a protective effect on liver and kidney functions of T. evansi-infected adult male Wistar rats.

Keywords

trypanosomescurcuminnanoparticlesoxidative stress

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Type
Research Article
Information
Parasitology , Volume 142 , Issue 3 , March 2015 , pp. 439 - 448
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Copyright © Cambridge University Press 2014 

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