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Small subunit rDNA phylogeny of Bacillidium sp. (Microspora, Mrazekiidae) infecting oligochaets

Published online by Cambridge University Press:  01 June 1999

F. NILSEN
F. NILSEN
Affiliation:
Department of Fisheries and Marine Biology, University of Bergen, Bergen High Technology Centre, N-5020 Bergen, Norway Present address: Institute of Marine Research, Department of Aquaculture, P.O. Box 1870, N-5024 Bergen, Norway. Tel: +47 55 23 63 02. Fax: +47 55 23 63 79. E-mail: frank.nilsen@imr.no
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Abstract

Small subunit (SSU) rDNA has been sequenced from a microsporidium, identified as a member of the genus Bacillidium obtained from an oligochaete. The length of the amplified PCR product was 1386 bp which is currently the longest microsporidium SSU sequence known. Phylogenetic analysis using 28 microsporidia SSU sequences, using 3 different tree-building methods indicated that Bacillidium sp. may be one of the earliest branches on the microsporidia tree. However, bootstrapping failed to give a high score (more than 50%) for the position of Bacillidium sp. The branch leading to Bacillidium sp. was long, indicating that this species is not closely related to any of the other microsporidia so far studied by means of rDNA.

Keywords

BacillidiumMicrosporamolecular phylogenytaxonomy

Information

Type
Research Article
Information
Parasitology , Volume 118 , Issue 6 , June 1999 , pp. 553 - 558
Copyright
1999 Cambridge University Press

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