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Pathogenic characterization in mice of Neospora caninum isolates obtained from asymptomatic calves

Published online by Cambridge University Press:  17 March 2010

D. PEREIRA GARCÍA-MELO ,
J. REGIDOR-CERRILLO ,
E. COLLANTES-FERNÁNDEZ ,
A. AGUADO-MARTÍNEZ ,
I. DEL POZO ,
E. MINGUIJÓN ,
M. GÓMEZ-BAUTISTA ,
G. ADURIZ  and
L. M. ORTEGA-MORA
D. PEREIRA GARCÍA-MELO
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040Madrid, Spain
J. REGIDOR-CERRILLO*
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040Madrid, Spain
E. COLLANTES-FERNÁNDEZ
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040Madrid, Spain
A. AGUADO-MARTÍNEZ
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040Madrid, Spain
I. DEL POZO
Affiliation:
NEIKER-Tecnalia, Instituto Vasco de Investigación y Desarrollo Agrario, Derio, 48160, Bizkaia, Spain
E. MINGUIJÓN
Affiliation:
NEIKER-Tecnalia, Instituto Vasco de Investigación y Desarrollo Agrario, Derio, 48160, Bizkaia, Spain
M. GÓMEZ-BAUTISTA
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040Madrid, Spain
G. ADURIZ
Affiliation:
NEIKER-Tecnalia, Instituto Vasco de Investigación y Desarrollo Agrario, Derio, 48160, Bizkaia, Spain
L. M. ORTEGA-MORA
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040Madrid, Spain
*
*Corresponding author: SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040-Madrid, Spain. Tel: +34 91 3944069. Fax: +34 91-944098. E-mail: jregidor@vet.ucm.es
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Summary

In this study, we characterized 8 new isolates obtained from healthy but congenitally infected calves using a BALB/c mouse model. Neospora caninum-infected mice survived without exhibiting any clinical signs of disease. Nevertheless, differences among isolates in parasite organ distribution, parasite burden and the severity of histopathological lesions were determined. Mice infected with the Nc-Spain 5H, Nc-Spain 7 and Nc-Spain 9 isolates showed higher parasite burdens and more severe brain lesions during the late phase of infection compared to mice infected with the Nc-Spain 2H, Nc-Spain 3H or Nc-Spain 6 isolates. Furthermore, differences in the immunoglobulin IgG1 and IgG2a isotype kinetics induced by these isolates were observed, with a more rapid IgG2a response seen in mice infected with the Nc-Spain 2H and Nc-Spain 3H isolates. These results confirm the intra-species variability of N. caninum pathogenicity.

Keywords

Neospora caninumbovine isolatespathogenic characterizationmice

Information

Type
Research Article
Information
Parasitology , Volume 137 , Issue 7 , June 2010 , pp. 1057 - 1068
Copyright
Copyright © Cambridge University Press 2010

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