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Molecular characterization and phylogenetic analysis of ascarid nematodes from twenty-one species of captive wild mammals based on mitochondrial and nuclear sequences

Published online by Cambridge University Press:  01 May 2012

YAN LI ,
LILI NIU ,
QIANG WANG ,
ZHIHE ZHANG ,
ZHIGANG CHEN ,
XIAOBIN GU ,
YUE XIE ,
NING YAN ,
SHUXIAN WANG  and
XUERONG PENG
...Show all authors
YAN LI
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
LILI NIU
Affiliation:
Veterinary Hospital, Chengdu Zoological Garden, Chengdu, Sichuan 610081, China
QIANG WANG
Affiliation:
Veterinary Hospital, Chengdu Zoological Garden, Chengdu, Sichuan 610081, China
ZHIHE ZHANG
Affiliation:
The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife – Developing toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan 610081, China
ZHIGANG CHEN
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
XIAOBIN GU
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
YUE XIE
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
NING YAN
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
SHUXIAN WANG
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
XUERONG PENG
Affiliation:
Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an 625014, China
GUANGYOU YANG*
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
*
*Corresponding author: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China. Tel: +86 835 2882787. Fax: +86 835 2885302. E-mail: guangyou1963@yahoo.com.cn
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Summary

Although ascarid nematodes are important parasites of wild animals of public health concern, few species of ascarids from wild animals have been studied at the molecular level so far. Here, the classification and phylogenetic relationships of roundworms from 21 species of captive wild animals have been studied by sequencing and analysis of parts of the ribosomal 18S and 28S genes and the mitochondrial (mt) 12S gene. Phylogenetic relationships were inferred by 3 methods (NJ/MP/ML) based on the data of single gene sequences and concatenated sequences. Homology analysis indicated that the 18S sequences were conserved among roundworms from all 21 species and that 28S showed interspecies variability. Divergence levels displayed in 12S suggested that 12S appears to be either intra- or interspecifically variable. Evolutionary trees indicated that the ascarids split into 2 families, 4 genera and 7 species, with high bootstrap support for each clade. Combined trees suggested that Baylisascaris ailuri is more closely related to B. transfuga than to B. schroederi. This study provides useful molecular markers for the classification, phylogenetic analysis and epidemiological investigation of roundworms from wild animals.

Keywords

giant pandawild mammalsAscarid nematodesmitochondrial genenuclear geneclassificationmolecular phylogeny

Information

Type
Research Article
Information
Parasitology , Volume 139 , Issue 10 , September 2012 , pp. 1329 - 1338
Copyright
Copyright © Cambridge University Press 2012

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