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A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues

Published online by Cambridge University Press:  01 July 1997

J. P. DALTON ,
S. R. DAY ,
A. C. DREW  and
P. J. BRINDLEY
J. P. DALTON
Affiliation:
Molecular Parasitology Unit, The Queensland Institute of Medical Research, The Bancroft Centre, Post Office, Royal Brisbane Hospital, Qld. 4029, and The Australian Centre for International and Tropical Health and Nutrition, Australia School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
S. R. DAY
Affiliation:
Molecular Parasitology Unit, The Queensland Institute of Medical Research, The Bancroft Centre, Post Office, Royal Brisbane Hospital, Qld. 4029, and The Australian Centre for International and Tropical Health and Nutrition, Australia
A. C. DREW
Affiliation:
Molecular Parasitology Unit, The Queensland Institute of Medical Research, The Bancroft Centre, Post Office, Royal Brisbane Hospital, Qld. 4029, and The Australian Centre for International and Tropical Health and Nutrition, Australia
P. J. BRINDLEY
Affiliation:
Molecular Parasitology Unit, The Queensland Institute of Medical Research, The Bancroft Centre, Post Office, Royal Brisbane Hospital, Qld. 4029, and The Australian Centre for International and Tropical Health and Nutrition, Australia
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Abstract

A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure sufficient quantities of miracidia were obtained to generate a cDNA library. Southern blot analysis demonstrated that miracidia isolated by this method were free from contaminating host DNA.

Keywords

Schistosoma mansoniSchistosoma japonicumeggsmiracidia

Information

Type
Research Article
Information
Parasitology , Volume 115 , Issue 1 , July 1997 , pp. 29 - 32
Copyright
1997 Cambridge University Press

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