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Cordycepin (3′-deoxyadenosine) pentostatin (deoxycoformycin) combination treatment of mice experimentally infected with Trypanosoma evansi

Published online by Cambridge University Press:  29 January 2013

LUCIANA DALLA ROSA ,
ALEKSANDRO S. DA SILVA ,
LUCAS T. GRESSLER ,
CAMILA B. OLIVEIRA ,
MARIA G. C. DAMBRÓS ,
LUIZ C. MILETTI ,
RAQUELI T. FRANÇA ,
SONIA T. A. LOPES ,
YASMIN N. SAMARA  and
MARCELO L. DA VEIGA
...Show all authors
LUCIANA DALLA ROSA*
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
ALEKSANDRO S. DA SILVA
Affiliation:
Department of Animal Science, Universidade do Estado de Santa Catarina, Chapecó, SC, Brazil
LUCAS T. GRESSLER
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
CAMILA B. OLIVEIRA
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
MARIA G. C. DAMBRÓS
Affiliation:
Department of Animal Production, Universidade do Estado de Santa Catarina, Lages, SC, Brazil
LUIZ C. MILETTI
Affiliation:
Department of Animal Production, Universidade do Estado de Santa Catarina, Lages, SC, Brazil
RAQUELI T. FRANÇA
Affiliation:
Department of Small Animals, Universidade Federal de Santa Maria, Brazil
SONIA T. A. LOPES
Affiliation:
Department of Small Animals, Universidade Federal de Santa Maria, Brazil
YASMIN N. SAMARA
Affiliation:
Department of Morphology, Universidade Federal de Santa Maria, Brazil
MARCELO L. DA VEIGA
Affiliation:
Department of Morphology, Universidade Federal de Santa Maria, Brazil
SILVIA G. MONTEIRO
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
*
* Corresponding author: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil. E-mail: ludallarosa@hotmail.com
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Summary

The aim of this study was to evaluate the anti-trypanosomal effect of treatment with 3′-deoxyadenosine (cordycepin) combined with deoxycoformycin (pentostatin: inhibitor of the enzyme adenosine deaminase) in vitro by using mice experimentally infected with Trypanosoma evansi. In vitro, a dose-dependent trypanocidal effect of cordycepin was observed against the parasite. In the in vivo trials, the two drugs were used individually and in combination of different doses. The drugs when used individually had no curative effect on infected mice. However, the combination of cordycepin (2 mg kg−1) and pentostatin (2 mg kg−1) was 100% effective in the T. evansi-infected groups. There was an increase in levels of some biochemical parameters, especially on liver enzymes, which were accompanied by histological lesions in the liver and kidneys. Based on these results we conclude that treatment using the combination of 3′-deoxyadenosine with deoxycoformycin has a curative effect on mice infected with T. evansi. However, the therapeutic protocol tested led to liver and kidney damage, manifested by hepatotoxicity and nephrotoxicity.

Keywords

trypanosomiasisadenosineadenosine deaminase inhibitor

Information

Type
Research Article
Information
Parasitology , Volume 140 , Issue 5 , April 2013 , pp. 663 - 671
Copyright
Copyright © Cambridge University Press 2013

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