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Cellular infiltration at skin lesions and draining lymph nodes of sheep infested with adult Hyalomma anatolicum anatolicum ticks

Published online by Cambridge University Press:  15 July 2005

D. K. V. BOPPANA ,
S. K. WIKEL ,
D. G. RAJ ,
M. B. MANOHAR  and
J. LALITHA
D. K. V. BOPPANA
Affiliation:
Departments of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 500007, India
S. K. WIKEL
Affiliation:
Center for Microbial Pathogenesis, School of Medicine, University of Connecticut Health Center, 263 Farmington Avenue, MC3710, Farmington, CT 06030, USA
D. G. RAJ
Affiliation:
Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 500007, India
M. B. MANOHAR
Affiliation:
Veterinary Pathology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 500007, India
J. LALITHA
Affiliation:
Departments of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 500007, India
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Abstract

Immunohistochemical analysis of skin and draining lymph nodes of sheep repeatedly infested with the ixodid tick Hyalomma anatolicum anatolicum were studied for different antigen-presenting cells and lymphocyte subpopulations. Infiltration of neutrophils, macrophages and lymphocytes adjacent to the tick bite site were observed. Skin biopsies showed significant increases in dermal infiltration of CD8+ and γδ+ T cells at 72 h and 8 days after both primary and secondary infestation. Infiltrations of MHC-II DR/DQ decreased at 72 h after tick infestation, whereas significant increases were recorded for 8-day skin biopsies. CD1+ cellular infiltrations were observed during secondary infestations at the dermis. Decreased ratios of CD4[ratio ]CD8 T cells and MHC-II[ratio ]CD1 antigen-presenting cells were observed in both infestations compared to healthy skin biopsies. Ratios of αβ[ratio ]γδ T cells increased gradually during infestation compared to uninfested skin. The regional lymph nodes from tick-infested sheep showed an increased CD8+, γδ+ T and CD1+ cellular infiltration compared to control lymph nodes. CD4+ T cells were decreased. There were no significant changes in CD45R+ cellular infiltration either at skin lesions or regional lymph nodes.

Keywords

Hyalomma anatolicum anatolicumixodid tickimmunomodulationsheeplymphocytesantigen-presenting cells

Information

Type
Research Article
Information
Parasitology , Volume 131 , Issue 5 , November 2005 , pp. 657 - 667
Copyright
© 2005 Cambridge University Press

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