Hostname: page-component-7dd5485656-2pp2p Total loading time: 0 Render date: 2025-10-30T14:35:10.587Z Has data issue: false hasContentIssue false
  • English
  • Français

A new method for the partial purification of leishmania amastigotes from cutaneous lesions

Published online by Cambridge University Press:  06 April 2009

R. B. Infante ,
A. G. Hernandez ,
F. Riggione  and
K. Dawidowicz
R. B. Infante
Affiliation:
Facultad de Ciencias, Universidad Central de Venezuela, Apartado 10098, Caracas, Venezuela
A. G. Hernandez
Affiliation:
Facultad de Ciencias, Universidad Central de Venezuela, Apartado 10098, Caracas, Venezuela
F. Riggione
Affiliation:
Instituto Venezolano de Investigaciones Cientificas, Venezuela
K. Dawidowicz
Affiliation:
Facultad de Ciencias, Universidad Central de Venezuela, Apartado 10098, Caracas, Venezuela
Get access Rights & Permissions [Opens in a new window]

Summary

Amastigotes from Leishmania braziliensis and L. mexicana were isolated from cutaneous lesions in infected animals using the plant lectin Concanavalin A as a specific agglutination agent. Amastigotes were collected in preparations of up to 95% purity as determined by cell count. The parasites obtained by this method showed no apparent loss of viability as measured by growth rates and DNA replication, or pathogenicity as measured by routine passages in hamsters or mice.

Information

Type
Research Article
Information
Parasitology , Volume 80 , Issue 1 , February 1980 , pp. 105 - 112
Copyright
Copyright © Cambridge University Press 1980

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Akiyama, H. J. & McQuillen, N. K. (1972). Interaction and transformation of Leishmania donovani within in vitro cultured cells. An electron microscopical study. American Journal of Tropical Medicine and Hygiene. 21, 873–9.CrossRefGoogle ScholarPubMed
Akiyama, H. J. & Taylor, J. C. (1970). Effect of macrophage engulfment and temperature on the transformation process of Leishmania donovani. American Journal of Tropical Medicine and Hygiene 19, 747–54.CrossRefGoogle ScholarPubMed
Bhattacharya, A. & Janovy, J. Jr. (1975). Leishmania donovani:autoradiographic evidence for molecular exchanges between parasites and host cells. Experimental Parasitology. 37, 353–60.CrossRefGoogle ScholarPubMed
Chance, M. L., Peters, W. & Shchory, L. (1974). Biochemical taxonomy of Leishmania I. Observations on DNA. Annals of Tropical Medicine and Parasitology. 68, 307–16.CrossRefGoogle ScholarPubMed
Childs, G. E., McRoberts, M. J. & Foster, K. A. (1976). Partial purification of amastigotes from cutaneous lesions of American Leishmaniasis. Journal of Parasitology. 62, 676–9.CrossRefGoogle ScholarPubMed
Dawidowicz, K., Hern´ndez, A. G., Infante, R. B. & Convit, J. (1975). The surface membrane of Leishmania I. The effects of lectins on different stages of Leishmania braziliensis. Journal of Parasitology. 61, 950–3.Google Scholar
Greenblatt, C. L. & Glaser, P. (1965). Temperature effect on Leishmania enrietti in vitro. Experimental Parasitology. 16, 36–42.CrossRefGoogle ScholarPubMed
Gutteridge, W. E., Cover, B. & Gaborak, M. (1978). Isolation of blood and intracellular forms of Trypanosoma cruzi from rats and other rodents, and preliminary studies of their metabolism. Parasitology. 76, 159–76.CrossRefGoogle ScholarPubMed
Janovy, J. Jr. (1967). Respiratory changes accompanying leishmania leptptomonad transformation in Leishmania donovani. Experimental Parasitology. 20, 51–5.CrossRefGoogle ScholarPubMed
Kirby, K. S. (1957). A new method for the isolation of deoxyribonucleic acids. Evidence of the nature of bonds between deoxyribonucleic acid and protein. Biochemical Journal. 66, 495–504.CrossRefGoogle Scholar
Laurent, M., Van Assel, S. & Steinert, M. (1971). Kinetoplast DNA. A unique macromolecular structure of considerable size and mechanical resistance. Biochemical and Biophysical Research Communications. 43, 278–84.CrossRefGoogle ScholarPubMed
Lamy, L. M. (1969). La Transformation réciproque des formes mastigotes et Amastigotes de Leishmania et son déterminisme en présence de cellules vivants in vitro. Annales de l'Institut Pasteur, Paris 117, 545–55.Google Scholar
Lemma, A. & Schiller, E. L. (1964). Extracellular cultivation of the leishmanial bodies of species belonging to the protozoan genus Leishmania. Experimental Parasitology. 15, 503–13.CrossRefGoogle Scholar
Schildkraut, C., Marmur, J. & Doty, P. (1962). Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. Journal of Molecular Biology. 4, 430–43.CrossRefGoogle ScholarPubMed
Simpson, L. (1968). The leishmania–leptomonad transformation of Leishmania donovani: nutritional requirements, respiratory changes and antigenic changes. Journal of Protozoology. 15, 201–7.CrossRefGoogle Scholar
Trager, W. (1953). The development of Leishmania donovani in vitro at 37 °C. Effects of the kind of serum. Journal of Experimental Medicine. 97, 177–88.CrossRefGoogle ScholarPubMed
Ulrich, M., Trujillo, D. & Convit, J. (1968). The effects of fresh serum on the leptomonads of Leishmania. I. A preliminary report. Transactions of the Royal Society of Tropical Medicine and Hygiene. 62, 825–30.CrossRefGoogle Scholar