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Sequence analysis of the 5·8S rDNA and ITS regions in evaluating genetic relationships among some species of Hypoxylon and related genera

Published online by Cambridge University Press:  12 July 2001

Angelo MAZZAGLIA ,
Naldo ANSELMI ,
Saverio VICARIO  and
Andrea VANNINI
Angelo MAZZAGLIA
Affiliation:
Department of Plant Protection, University of Studies of Tuscia, via S. Camillo de Lellis, 01100 Viterbo, Italy
Naldo ANSELMI
Affiliation:
Department of Plant Protection, University of Studies of Tuscia, via S. Camillo de Lellis, 01100 Viterbo, Italy
Saverio VICARIO
Affiliation:
Department of Biology, University Tor Vergata, via Orazio Raimondo, 00173 Roma, Italy. E-mail: vannini@unitus.it
Andrea VANNINI
Affiliation:
Department of Plant Protection, University of Studies of Tuscia, via S. Camillo de Lellis, 01100 Viterbo, Italy
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Abstract

Species belonging to Hypoxylon and related genera are bark pathogens and wood decay agents of angiosperms, sometimes able to live asymptomatically in host tissues. In the present work the ITS regions of the ribosomal DNA from 21 isolates belonging to five species were sequenced and then compared with each other. A phylogenetic parsimony analysis among isolates was carried out. ITS2 appears to be the most informative among the sequenced portions of rDNA. Results are strictly consistent with the latest taxonomic arrangements obtained with classical morphological approaches. The ITS sequence analysis is an efficient and accurate method for discriminating among taxa of Hypoxylon and related genera.

Information

Type
Research Article
Information
Mycological Research , Volume 105 , Issue 6 , June 2001 , pp. 670 - 675
Copyright
© The British Mycological Society 2001

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