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Sub-micron Patterning on Polymer Films for Protein Arrays

Published online by Cambridge University Press:  01 February 2011

Karen L. Christman ,
Michael V. Requa ,
Vanessa D. Enriquez-Rios ,
Paula Mendes ,
J. Fraser Stoddart ,
Kimberley L. Turner  and
Heather D. Maynard
Karen L. Christman
Affiliation:
christman@chem.ucla.edu, University of California Los Angeles, 607 Charles E. Young Dr. East, Los Angeles, CA, 90095-1569, United States
Michael V. Requa
Affiliation:
requa@engineering.ucsb.edu
Vanessa D. Enriquez-Rios
Affiliation:
ver@chem.ucla.edu
Paula Mendes
Affiliation:
mendes@chem.ucla.edu
J. Fraser Stoddart
Affiliation:
stoddart@chem.ucla.edu
Kimberley L. Turner
Affiliation:
turner@engineering.ucsb.edu
Heather D. Maynard
Affiliation:
maynard@chem.ucla.edu

Abstract

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Patterning proteins at the sub-micron and nanoscale has many uses, including fabrication of protein arrays for diagnostic and sensor applications. Many groups have reported micron scale protein patterns using photolithography; however, smaller scales have not been realized. In this study, we demonstrate sub-micron protein patterns using photolithography. Patterning is achieved by chemical transformation of pH-reactive polymer films. Site-specific immobilization of streptavidin within a protein resistant background is demonstrated. This methodology could be utilized for the development of high density proteins arrays for biotechnology applications.

Keywords

polymerfilmnanoscale
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 900: Symposium O – Nanoparticles and Nanostructures in Sensors and Catalysis , 2005 , 0900-O09-35
Copyright
Copyright © Materials Research Society 2006

References

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