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Site-Specific Attachment of Gold Nanoparticles to DNA Templates

Published online by Cambridge University Press:  17 March 2011

Karen A. Stevenson ,
Govindarajan Muralidharan ,
Leon Maya ,
Jack C. Wells  and
Jacob Barhen
Karen A. Stevenson
Affiliation:
Thomas Thundat Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831
Govindarajan Muralidharan
Affiliation:
Thomas Thundat Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831
Leon Maya
Affiliation:
Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831
Jack C. Wells
Affiliation:
Computer Science and Mathematics Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831
Jacob Barhen
Affiliation:
Computer Science and Mathematics Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831
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Abstract

DNA was used as a scaffold for the binding of gold nanoparticles using a standard chemical technique. A DNA template was designed with amino-modified thymines located every 3.7 nm, which would allow the attachment of the carboxylic acid functionalized gold nanoparticles. The gold particles were covalently bound to the amino groups on the DNA using standard 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) chemistry in the presence of a competitor to block excess gold binding sites. The products were analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM).

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