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Preparation of Liquids and Colloidal Dispersions for Tem: Cryo-Sem Studies of Bare-Film and Freeze-Fracture Specimens

Published online by Cambridge University Press:  21 February 2011

J. R. Bellare ,
J. G. Sheehan ,
H. T. Davis  and
L. E. Scriven
J. R. Bellare
Affiliation:
Department of Chemical Engineering and Materials Science, University Minnesota, Minneapolis, MN 55455-0132, U.S.A.
J. G. Sheehan
Affiliation:
Department of Chemical Engineering and Materials Science, University Minnesota, Minneapolis, MN 55455-0132, U.S.A.
H. T. Davis
Affiliation:
Department of Chemical Engineering and Materials Science, University Minnesota, Minneapolis, MN 55455-0132, U.S.A.
L. E. Scriven
Affiliation:
Department of Chemical Engineering and Materials Science, University Minnesota, Minneapolis, MN 55455-0132, U.S.A.
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Abstract

Liquid and colloidal microstructures can be imaged at high resolution with cryo-TEM and cryo-SEM by fast-freezing with a Controlled Environment Vitrification System. Frozen bare-films can be directly imaged by TEM, while films sandwiched between metal plates can be frozen, fractured, and directly imaged with cryo-SEM or indirectly imaged by TEM after replication. We describe sample preparation techniques and report cryo-SEM studies of particle distributions and fracture patterns in such specimens.

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References

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