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Plasma Protein Fractionation by Membrane Filtration in Therapeutic Plasmapheresis

Published online by Cambridge University Press:  26 February 2011

Takashi Horiuchi ,
Paul S. Malchesky  and
Yukihiko Nosé
Takashi Horiuchi
Affiliation:
The Cleveland Clinic Foundation, Department of Artificial Organs, 9500 Euclid Avenue, Cleveland, OH 44106
Paul S. Malchesky
Affiliation:
The Cleveland Clinic Foundation, Department of Artificial Organs, 9500 Euclid Avenue, Cleveland, OH 44106
Yukihiko Nosé
Affiliation:
The Cleveland Clinic Foundation, Department of Artificial Organs, 9500 Euclid Avenue, Cleveland, OH 44106
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Abstract

Synthetic polymer membranes which have a nominal pore size (NPS) of 0.03 to 10 microns were evaluated at varying filtration temperatures to separate pathologic macromolecules such as immune complexes, cryoprecipitable proteins and low density lipoproteins (LDL) from essential plasma proteins. At low temperatures (4 to 10°C) cryoprecipitable proteins were selectively removed from cryoglobulinemic plasmas with membranes of 3 micron NPS. However, severe membrane plugging occurred with membranes of smaller pore sizes. In addition, decreased albumin sieving occurs. Above 37°C, increased albumin sieving and prolonged membrane plugging occurs. In the selective removal of low density lipoproteins from hypercholesterolemic plasmas, membranes with NPS of 0.03 microns were found to be suitable at high operating temperatures. Given the proper combination of membrane and temperature control, disease related pathological macromolecules can be treated effectively by selective macromolecule removal.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 110: Symposium M – Biomedical Materials and Devices , 1987 , 755
Copyright
Copyright © Materials Research Society 1988

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References

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