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Osteoblast Response to Amino Acid-modified Surfaces

Published online by Cambridge University Press:  01 February 2011

Simon Todd ,
Rachel Rawsterne ,
Rein Ulijn  and
Julie Gough
Simon Todd
Affiliation:
simon.todd@postgrad.manchester.ac.uk, University of Manchester, Materials Science, Grosvenor Street, Manchester, M1 7HS, United Kingdom
Rachel Rawsterne
Affiliation:
rachel_rawsterne@hotmail.com, University of Manchester, Materials Science, Grosvenor Street, Manchester, M1 7HS, United Kingdom
Rein Ulijn
Affiliation:
rein.ulijn@manchester.ac.uk, University of Manchester, Materials Science, Grosvenor Street, Manchester, M1 7HS, United Kingdom
Julie Gough
Affiliation:
julie.gough@manchester.ac.uk, University of Manchester, Materials Science, Grosvenor Street, Manchester, M1 7HS, United Kingdom
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Abstract

The behavior of cells at the material surface is of critical importance in the design of biomaterials. To understand and control cell responses to surfaces, different surface chemistries are commonly compared. In this study we exploit the chemical diversity of the 20 naturally occurring amino acids to make 20 surfaces with different chemical properties. The biological response of osteoblasts to these surfaces was examined. The osteoblast response to the surfaces was found to be dependant on the wettability of the surface with polar amino acid surfaces (Cys<Ser<Thr<Gln<Asn) promoting osteoblast spreading more than hydrophobic aromatic amino acid surfaces (Phe <Tyr <Trp), aliphatic amino acid surfaces (Leu<Ile<Val<Ala<Met<Gly), and Pro. The surface charge also affected the cellular response with positively charged amino acid surfaces (His<Arg<Lys) producing a higher percentage of spreading cells compared to the negatively charged surfaces (Asp<Glu). Amino acid surfaces provide a range of well defined chemical functionalities that are useful in studying the interactions of cells with material surfaces.

Keywords

biomedicalbiomaterialbiological
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 950: Symposium D – Biosurfaces and Biointerfaces , 2006 , 0950-D04-20
Copyright
Copyright © Materials Research Society 2007

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