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Oriented Collagen Matrices: the Control of Biomineralizaton in Bone

Published online by Cambridge University Press:  15 February 2011

Osamu Nakamura ,
David J. Fink  and
Arnold I. Caplan
Osamu Nakamura
Affiliation:
Skeletal Research Center, Department of Biology, Case Western Reserve University, Cleveland, OH 44106
David J. Fink
Affiliation:
CollaTek, Inc., 1445 Summit St., Columbus, OH 43201
Arnold I. Caplan
Affiliation:
Skeletal Research Center, Department of Biology, Case Western Reserve University, Cleveland, OH 44106
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Abstract

Bone-forming cells fabricate a highly ordered collagen matrix (osteoid) which subsequently mineralizes. A variety of cell culture systems exist for osteogenic cells, yet none of these is optimal for the organized formation of a mineralized matrix. We have generated collagen substrates which have different degrees of fibrillar orientation, and have cultured osteogenic cells on these matrices. In this format, von Kossa-stained sections show that highly oriented collagen matrix starts to calcify in 6–7 days, while a random fibrillar matrix does not mineralize even after 21 days. Mineral has been detected only within the collagen matrix with a narrow, unmineralized region between the cells and the mineral.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 218: Symposium V – Materials Synthesis Based on Biological Processes , 1990 , 275
Copyright
Copyright © Materials Research Society 1991

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References

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