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Ink Jet printing of mammalian primary cells for tissue engineering applications

Published online by Cambridge University Press:  01 February 2011

Rachel Saunders ,
Julie Gough  and
Brian Derby
Rachel Saunders
Affiliation:
Materials Science Centre, The University of Manchester, Grosvenor Street, Manchester, M1 7HS
Julie Gough
Affiliation:
Materials Science Centre, The University of Manchester, Grosvenor Street, Manchester, M1 7HS
Brian Derby
Affiliation:
Materials Science Centre, The University of Manchester, Grosvenor Street, Manchester, M1 7HS
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Abstract

A piezoelectric drop on demand printer has been used to print primary human osteoblast and bovine chondrocyte cells. After deposition the cells were incubated at 37°C and characterised using optical microscopy, SEM and cell viability assays. Cells showed a robust response to printing exhibiting signs of proliferation and spreading. Increasing the drop velocity results in a reduced cell survival and proliferation rates but both cell types grew to confluence after printing under all conditions studied.

Information

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA2.8
Copyright
Copyright © Materials Research Society 2005

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References

REFERENCES

1. Leong, K.F., Cheah, C.M. and Chua, C.K., “Solid freeform fabrication of three-dimensional scaffolds for engineering replacement tissues and organs”, Biomaterials 24, 2363–2378, 2003 Google Scholar
2. Hutmacher, D.W., Sittinger, M. and Risbud, M.V., “Scaffold-based tissue engineering: rationale for computer aided design and solid free-form fabrication systems”, Trends in biotechnology 22, 354–362, 2004 Google Scholar
3. Sachlos, E. and Czernuszka, J.T., “Making tissue engineering scaffolds work. Review on the application of solid freeform fabrication technology to the production of tissue engineering scaffolds”, European cells and materials, 5, 29–40, 2003 Google Scholar
4. Freed, L.E. and Vunjak-Novakovic, G., “Culture of organised cell communities”, Advanced drug delivery reviews, 33, 15–39, 1998 Google Scholar
5. Wendt, D., Marsano, A., Jakob, M., Heberer, M. and Martin, I., “Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity”, Biotechnology and Bioengineering, 84, 205–214, 2003 Google Scholar
6. Xu, T., Jin, J., Gregory, C., Hickman, J.J., and Boland, T., “Inkjet printing of viable mammalian cells”, Biomaterials, 26, 93–99, 2005 Google Scholar
7. Saunders, R., Bosworth, L., Gough, J., Derby, B., and Reis, N., “Selective cell delivery for 3D tissue culture and engineering”, European Cells and Materials, 7, Suppl 1, 84–85, 2004 Google Scholar