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Gold Nanoparticle Enlargement Coupled with Fluorescence Decreasefor Highly Sensitive Detection of Analytes

Published online by Cambridge University Press:  28 January 2011

Seong Yoon Lim ,
Jae Hong Kim ,
Joon Seok Lee  and
Chan Beum Park
Seong Yoon Lim
Affiliation:
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology 335 Science Road, Daejeon 305-701, Republic of Korea
Jae Hong Kim
Affiliation:
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology 335 Science Road, Daejeon 305-701, Republic of Korea
Joon Seok Lee
Affiliation:
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology 335 Science Road, Daejeon 305-701, Republic of Korea
Chan Beum Park
Affiliation:
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology 335 Science Road, Daejeon 305-701, Republic of Korea
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Abstract

We present a versatile and facile route for highly sensitive detection ofanalytes through coupling the enlargement of gold nanoparticles (Au NPs)with fluorescence decrease. The fluorescence intensity of dye molecules(e.g., fluorescein or rhodamine B) significantly decreased with theincreasing concentration of reducing agents, such as hydrogen peroxide andhydroquinone. The sensitivity for the detection of reducing agents was muchhigher than other detection methods based on the absorbance measurement ofenlarged gold nanoparticles or quantum dot-enzyme hybridization. We couldsuccessfully detect acetylthiocholine with the detection limit of several nMorders, using an enzymatic reaction by acetylcholinesterase, a key route forthe detection of toxic organophosphate compounds. The fluorescencedecreasing approach described in this work requires only a simple additionof fluorescence dye to the reaction solution without any chemicalmodification. The strategy of fluorescence decrease coupled withnanoparticle growth will be applied on the fluorescent substrate to developdetection templates for highly sensitive optical biosensor.

Keywords

sensorAutransmission electron microscopy (TEM)

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References

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