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Electron-beam Directed Layer-by-Layer Assembly of Dendrimer Scaffold for Biomolecule Patterning

Published online by Cambridge University Press:  01 February 2011

Parijat Bhatnagar ,
Sonny S Mark ,
Il Kim ,
Hongyu Chen ,
Brad Schmidt ,
Michal Lipson  and
Carl A Batt
Parijat Bhatnagar
Affiliation:
pb96@cornell.edu, Cornell University, Department of Biomedical Engineering, 309 Stocking Hall, Cornell University, Ithaca, NY, 14853, United States, 845-401-3427
Sonny S Mark
Affiliation:
ssm12@cornell.edu, Cornell University, Department of Microbiology, Ithaca, NY, 14853, United States
Il Kim
Affiliation:
ik58@cornell.edu, Cornell University, Department of Food Science, Ithaca, NY, 14853, United States
Hongyu Chen
Affiliation:
hc332@cornell.edu, Cornell University, Department of Food Science, Ithaca, NY, 14853, United States
Brad Schmidt
Affiliation:
bss15@cornell.edu, Cornell University, Department of Electrical and Computer Engineering, Ithaca, NY, 14853, United States
Michal Lipson
Affiliation:
lipson@ece.cornell.edu, Cornell University, Department of Electrical and Computer Engineering, Ithaca, NY, 14853, United States
Carl A Batt
Affiliation:
cab10@cornell.edu, Cornell University, Department of Biomedical Engineering, Microbiology, and Food Science, Ithaca, NY, 14853, United States
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Abstract

A method for patterning biomolecules using electron beam (e-beam) lithography has been developed. First, a non-biofouling poly (ethylene glycol) terminated self-assembled monolayer (SAM) was deposited on a silicon oxide substrate. This SAM was then ablated by e-beam to create patterns aligned with the pre-existing features on the wafer. Aldehyde-terminated polyamidoamine (PAMAM) dendrimers were assembled in a layer-by-layer fashion in the ablated patterns to allow the covalent immobilization of oligonucleotide probes. The aminated oligonucleotides were attached using Schiff base chemistry followed by reductive amination. The functionality of the attached oligonucleotides was demonstrated by the hybridization of fluorescently labeled complementary target oligonucleotides. The hybridized target oligonucleotides could be stripped and the regenerated surface bound probe oligonucleotides could be rehybridized with complementary target oligonucleotide.

Keywords

nanoscaleself-assemblysurface chemistry
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 921: Symposium T – Nanomanufacturing , 2006 , 0921-T04-08
Copyright
Copyright © Materials Research Society 2006

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