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Crystallization of Synthetic Hemozoin (Beta-Hematin) Nucleated at the Surface of Synthetic Neutral Lipid Bodies

Published online by Cambridge University Press:  01 February 2011

Timothy J Egan ,
Anh N Hoang ,
Kanyile K Ncokazi ,
Katherine A de Villiers  and
David W Wright
Timothy J Egan
Affiliation:
Timothy.Egan@uct.ac.za University of Cape Town Chemisty, Rondebosch, South Africa
Anh N Hoang
Affiliation:
anh.n.hoang@vanderbilt.edu Vanderbilt University Material Science, Nashville, Tennessee, United States
Kanyile K Ncokazi
Affiliation:
KANYILE.NCOKAZI@uct.ac.za University of Cape Town Chemisty, Rondebosch, South Africa
Katherine A de Villiers
Affiliation:
katherine.devilliers@uct.ac.za University of Stellenbosch Chemistry and Polymer Science, Stellenbosch, South Africa
David W Wright
Affiliation:
david.wright@vanderbilt.edu Vanderbilt University Chemistry, nashville, Tennessee, United States
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Abstract

The mechanism of formation of hemozoin, a detoxification by-product of several blood-feeding organisms including malaria parasites, has been a subject of debate; however, recent studies suggest that neutral lipids may serve as a catalyst. In this study, a model system consisting of an emulsion of synthetic lipid bodies, resembling their in vivo counterpart in composition and size, was employed to investigate the formation of β-hematin, synthetic hemozoin, at the lipid-water interface. The introduction of heme (Fe(III)PPIX) to this synthetic neutral lipid bodies system under biomimetic conditions (37°C, pH 4.8) produced beta-hematin with apparent first order kinetics and an average half life of 0.5 min. TEM of monoglycerides (MPG) extruded through a 200 nm filter with heme produced beta-hematin crystals aligned and parallel to the lipid/water interface. TEM data suggests that beta-hematin crystallizes via epitaxial nucleation at the lipid-water interface through interaction of Fe(III)PPIX with the polar head group and elongation occurs parallel this interface.

Keywords

Febiomaterialcrystal growth
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1274: Symposium QQ – Biological Materials and Structures in Physiologically Extreme Conditions and Disease , 2010 , 1274-QQ05-13
Copyright
Copyright © Materials Research Society 2010

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